scholarly journals Identification and transcriptional characterization of the gene encoding the stress-response σ factor σHinStreptomyces coelicolorA3(2)

2000 ◽  
Vol 189 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Ján Kormanec ◽  
Beatrica Å evčíková ◽  
Norika HalgaÅ¡ová ◽  
Renáta Knirschová ◽  
Bronislava Řežuchová
Keyword(s):  
Stress Response ◽  
Gene Encoding ◽  
Factor I ◽  
I Factor

scholarly journals Construction and Characterization of aHelicobacter pylori clpB Mutant and Role of the Gene in the Stress Response

1998 ◽  
Vol 180 (2) ◽  
pp. 426-429 ◽  
Author(s):  
Elaine Allan ◽  
Peter Mullany ◽  
Soad Tabaqchali
Keyword(s):  
Helicobacter Pylori ◽  
High Temperature ◽  
Stress Response ◽  
Structural Features ◽  
High Temperature Stress ◽  
Gene Encoding ◽  
Increased Sensitivity ◽  
H Pylori

ABSTRACT Antiserum raised against whole Helicobacter pyloricells identified a novel 94-kDa antigen. The nucleotide sequence of the gene encoding the 94-kDa antigen was determined, and analysis of the deduced amino acid sequence revealed structural features typical of the ClpB ATPase family of stress response proteins. An isogenic H. pylori clpB mutant showed increased sensitivity to high-temperature stress, indicating that the clpB gene product functions as a stress response protein in H. pylori.

Cloning and characterization of lipopolysaccharide-induced tumor necrosis factor α factor promoter

2006 ◽  
Vol 47 (3) ◽  
pp. 360-368 ◽  
Author(s):  
Nobuyuki Shiomi ◽  
Fumio Myokai ◽  
Koji Naruishi ◽  
Kosuke Oyaizu ◽  
Kyoko Senoo ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Factor I ◽  
I Factor ◽  
Necrosis Factor

scholarly journals NON-MENDELIAN FEMALE STERILITY IN DROSOPHILA MELANOGASTER: CHARACTERIZATION OF THE NONINDUCER CHROMOSOMES OF INDUCER STRAINS

Genetics ◽  
1979 ◽  
Vol 91 (3) ◽  
pp. 473-489
Author(s):  
Georges Picard ◽  
Alain Pelisson
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Recombination ◽  
Female Sterility ◽  
Chromosome 2 ◽  
Genetic Element ◽  
Mendelian Segregation ◽  
Factor I ◽  
I Factor ◽  
Males And Females

ABSTRACT In relation to non-Mendelian female sterility, Drosophila melanogaster strains can be divided into two main classes, inducer and reactive. The genetic element responsible for the inducer condition (I factor) is chromosomal and may be linked to any inducer-strain chromosome. Each chromosome carrying the I factor (i  + chromosome) can, when introduced by the paternal gamete into a reactive oocyte, give rise to females (denoted SF) showing more-or-less reduced fertility. As long as i  + chromosomes are transmitted through heterozygous males with reactive originating chromosomes (r chromosomes), I factor follows Mendelian segregation patterns. In contrast, in heterozygous i+/r females, a varying proportion of r chromosomes may irreversibly acquire I factor, independently of classical genetic recombination, by a process called chromosomal contamination. The contaminated reactive chromosomes behave as i  + chromosomes.—In the present paper, evidence is given that the Luminy inducer strain displays a polymorphism for two kinds of second chromosomes. Some of them are i  +, while others, denoted io, are unable t3 induce any SF sterility when introduced by paternal gametes into reactive oocytes. They are also unable to induce contamination of r chromosomes, but, like r chromosomes, they may be contaminated by i+ chromosomes in SF or RSF females. The study of the segregation of i  + and io second chromosomes in the progeny of heterozygous Luminy males and females leads to the conclusion that on chromosome 2 of the Luminy stock the I factor is at a single locus. —XI second and third io chromosomes have been found in several inducer strains. Since these chromosomes can be maintained with i  + chromosomes in inducer strains in spite of their ability to be contaminated in RSF females, it can be concluded that chromosomal contamination does not take place in females of inducer strains. This implies that contamination occurs only in cells having cytoplasm in a reactive state.

scholarly journals Isolation and characterization of extragenic suppressors of mutations in the SSA hsp70 genes of Saccharomyces cerevisiae.

Genetics ◽  
1992 ◽  
Vol 131 (2) ◽  
pp. 277-285 ◽  
Author(s):  
R J Nelson ◽  
M F Heschl ◽  
E A Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Regulatory Protein ◽  
Null Alleles ◽  
Temperature Sensitive ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Transcriptional Regulatory ◽  
Extragenic Suppressors

Abstract Saccharomyces cerevisiae strains that contain null alleles of two hsp70 genes, SSA1 and SSA2, are temperature sensitive for growth. In this study, extragenic suppressors of ssa1 ssa2 have been isolated. Suppression is due to mutations at nuclear loci designated EXA1, EXA2 and EXA3 for EXtragenic suppressor hsp70 subfamily A. Two of the four EXA1 alleles are dominant as is EXA3-1. The other two EXA1 alleles as well as the sole EXA2 allele are recessive. EXA1 mutations lead to accumulation of a previously uncharacterized form of hsp70. EXA2 and EXA3 mutations affect the regulation of the stress response. In exa2-1 ssa1 ssa2 strains the gene products of the remaining SSA hsp70 genes, SSA3 and SSA4 (Ssa3/4p), accumulate to higher levels. The EXA3-1 mutation results in increased accumulation of both Ssa3/4p and the hsp70s encoded by the SSB1 and SSB2 genes (Ssb1/2p), suggesting that the EXA3 gene product plays a central role in the yeast stress response. Consistent with this hypothesis, EXA3-1 is tightly linked to HSF1, the gene encoding the transcriptional regulatory protein known as "heat shock factor." All of the genes identified in this study seem to be involved in regulating the expression of SSA3 and SSA4 or the activity of their protein products.

scholarly journals Cloning and characterization of the gene encoding the primary σ-factor ofCampylobacter jejuni

1998 ◽  
Vol 162 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Marc M.S.M Wösten ◽  
Miranda Boeve ◽  
Wim Gaastra ◽  
Bernard A.M Zeijst
Keyword(s):  
Gene Encoding ◽  
Ofcampylobacter Jejuni ◽  
I Factor

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

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