EFFECT OF CROTON OIL APPLIED TO MOUSE SKIN ORIGINALLY PAINTED WITH SUBOPTIMAL DOSES OF CARCINOGEN DURING THE GROWTH AND RESTING PHASES OF THE HAIR FOLLICLES

In early postnatal mouse skin, the NG2 proteoglycan is expressed in the subcutis, the dermis, the outer root sheath of hair follicles, and the basal keratinocyte layer of the epidermis. With further development, NG2 is most prominently expressed by stem cells in the hair follicle bulge region, as also observed in adult human skin. During telogen and anagen phases of the adult hair cycle, NG2 is also found in stem cell populations that reside in dermal papillae and the outer root sheaths of hair follicles. Ablation of NG2 produces alterations in both the epidermis and subcutis layers of neonatal skin. Compared with wild type, the NG2 null epidermis does not achieve its full thickness due to reduced proliferation of basal keratinocytes that serve as the stem cell population in this layer. Thickening of the subcutis is also delayed in NG2 null skin due to deficiencies in the adipocyte population.

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Scanning Electrochemical Microscopy of Iontophoretic Transport in Hairless Mouse Skin. Analysis of the Relative Contributions of Diffusion, Migration, and Electroosmosis to Transport in Hair Follicles

Journal of Pharmaceutical Sciences ◽

10.1002/1520-6017(200012)89:12<1537::aid-jps4>3.0.co;2-j ◽

2000 ◽

Vol 89 (12) ◽

pp. 1537-1549 ◽

Cited By ~ 33

Author(s):

Bradley D. Bath ◽

Erik R. Scott ◽

J.Bradley Phipps ◽

Henry S. White

Keyword(s):

Mouse Skin ◽

Scanning Electrochemical Microscopy ◽

Hairless Mouse ◽

Hair Follicles ◽

Hairless Mouse Skin ◽

Diffusion Migration ◽

Electrochemical Microscopy

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EFFECTS OF STEROID HORMONES ON DEVELOPING MOUSE SKIN IN VITRO

Journal of Endocrinology ◽

10.1677/joe.0.0660195 ◽

1975 ◽

Vol 66 (2) ◽

pp. 195-205 ◽

Cited By ~ 4

Author(s):

AMREEK SINGH ◽

MARGARET H. HARDY

Keyword(s):

Cell Differentiation ◽

Organ Culture ◽

Sebaceous Gland ◽

Mouse Skin ◽

Hair Follicles ◽

Stratum Granulosum ◽

Normal Medium ◽

Hormonal Steroids ◽

Good Differentiation

SUMMARY Pieces of skin from 13·5- to 15-day-old foetal mice were grown in organ culture in a biological medium with or without the addition of hormonal steroids. Cortisol (7·5 μg/ml) caused thinning of the non-cornified epidermis and flattening of the stratum granulosum after 3 days. By 6 days the epidermis was thinner and hair follicles were regressing, and these changes continued up to 12 days. Administration of corticosterone (5 μg/ml) also produced thinning of the epidermis and regression of the follicles after 6 days. Good differentiation of epidermis and hair follicles was obtained when testosterone (100 μg/ml) was added to the medium. The non-cornified epidermal layers were similar to those of control cultures at 3 days but less than half as thick at 6 days. Hair follicles differentiated as rapidly in medium containing testosterone as in normal medium, but, unlike in the latter medium, also developed sebaceous gland anlagen at 6 days. Some explants in testosterone medium showed signs of sebaceous cell differentiation at 9 days.

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Differential Expression of a Cutaneous Corticotropin-Releasing Hormone System

Endocrinology ◽

10.1210/en.2003-0851 ◽

2004 ◽

Vol 145 (2) ◽

pp. 941-950 ◽

Cited By ~ 126

Author(s):

Andrzej Slominski ◽

Alexander Pisarchik ◽

Desmond J. Tobin ◽

Joseph E. Mazurkiewicz ◽

Jacobo Wortsman

Keyword(s):

Human Skin ◽

Expression Patterns ◽

Mouse Skin ◽

Hair Follicles ◽

Dermal Fibroblasts ◽

Preferential Expression ◽

Eccrine Glands ◽

Species Specific ◽

Skin Function ◽

Human And Mouse

Abstract We completed the mapping of a cutaneous CRH signaling system in two species with widely different determinants of skin functions, humans and mice. In human skin, the CRH receptor (CRH-R) 1 was expressed in all major cellular populations of epidermis, dermis, and subcutis with CRH-R1α being the most prevalent isoform. The CRH-R2 gene was expressed solely in hair follicle keratinocytes and papilla fibroblasts, whereas CRH-R2 antigen was localized predominantly in hair follicles, sebaceous and eccrine glands, muscle and blood vessels. In mouse skin, the CRH-R2 gene and protein were widely expressed in all cutaneous compartments and in cultured normal and malignant melanocytes. CRH-binding protein mRNA was present in dermal fibroblasts, melanoma cells, and sc fat of human skin and undetectable in mouse skin. The urocortin II gene was expressed equally in mouse and human skin. Taken together with our previous investigations, the present studies document the preferential expression of CRH-R1 in human skin, which mirrors CRH-R2 expression patterns in human and mouse skin. They are likely reflecting different functional activities of human and mouse skin. The adnexal location of CRH-R2 suggests a role for the receptor in hair growth. The differential interspecies CRH signaling expression pattern probably reflects adaptation to species-specific skin function determinants.

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Dicer- and BSC-dependent miRNAs during murine anagen development

10.1101/2020.01.12.903567 ◽

2020 ◽

Author(s):

Neda Vishlaghi ◽

Thomas S. Lisse

Keyword(s):

Hair Follicle ◽

Functional Role ◽

Rna Binding ◽

Mouse Skin ◽

Mature Mirnas ◽

Hair Follicles ◽

Conditional Knockout ◽

Follicle Cells ◽

Epidermal Keratinocytes ◽

Wide Range

AbstractMicroRNAs (miRNAs) are a major class of conserved non-coding RNAs that have a wide range of functions during development and disease. Biogenesis of canonical miRNAs depend on the cytoplasmic processing of pre-miRNAs to mature miRNAs by the Dicer endoribonuclease. Once mature miRNAs are generated, the miRNA-induced silencing complex, or miRISC, incorporates one strand of miRNAs as a template for recognizing complementary target messenger RNAs (mRNAs) to dictate post-transcriptional gene expression. Besides regulating miRNA biogenesis, Dicer is also part of miRISC to assist in activation of the complex. Dicer associates with other regulatory miRISC co-factors such as trans-activation responsive RNA-binding protein (Tarbp2) to regulate miRNA-based RNA interference. Although the functional role of miRNAs within epidermal keratinocytes have been extensively studied within embryonic and post-natal mouse skin, its contribution to the normal function of hair follicle bulge stem cells (BSCs) during post-natal hair follicle development is unknown. With this question in mind, we sought to ascertain whether Dicer-Tarpb2 plays a functional role within BSCs during induced anagen development by utilizing conditional knockout mouse models. Our findings suggest that Dicer, but not Tarbp2, functions within BSCs to regulate induced anagen (growth) development of post-natal hair follicles. These findings strengthen our understanding of miRNA-dependency within hair follicle cells to define a boundary for post-transcriptional gene regulation during anagen development.

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The Sulphydryl Levels of Mouse Skin During Treatment with Polycyclic Hydrocarbons and Croton Oil

British Journal of Cancer ◽

10.1038/bjc.1961.46 ◽

1961 ◽

Vol 15 (2) ◽

pp. 360-366 ◽

Cited By ~ 2

Author(s):

G Calcutt ◽

Joan Coates

Keyword(s):

Mouse Skin ◽

Polycyclic Hydrocarbons ◽

Croton Oil

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Mouse skin melanoma induced in two stage chemical carcinogenesis with 7, 12-dimethylbenz[a]anthracene and croton oil

Carcinogenesis ◽

10.1093/carcin/6.6.921 ◽

1985 ◽

Vol 6 (6) ◽

pp. 921-923 ◽

Cited By ~ 21

Author(s):

Haruo Takizawa ◽

Sei-ichi Sato ◽

Hirono Kitajima ◽

Sumie Konishi ◽

Kunio Iwata ◽

...

Keyword(s):

Mouse Skin ◽

Chemical Carcinogenesis ◽

Skin Melanoma ◽

Two Stage ◽

Croton Oil

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Histology and Ultrastructure of Transitional Changes in Skin Morphology in the Juvenile and Adult Four-Striped Mouse (Rhabdomys pumilio)

The Scientific World JOURNAL ◽

10.1155/2013/259680 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

Eranée Stewart ◽

Moyosore Salihu Ajao ◽

Amadi Ogonda Ihunwo

Keyword(s):

Adult Mouse ◽

Mouse Skin ◽

Hair Follicles ◽

Ultrastructural Analysis ◽

Rhabdomys Pumilio ◽

Mammalian Skin ◽

Skin Morphology ◽

Masson Trichrome Staining

The four-striped mouse has a grey to brown coloured coat with four characteristic dark stripes interspersed with three lighter stripes running along its back. The histological differences in the skin of the juvenile and adult mouse were investigated by Haematoxylin and Eosin and Masson Trichrome staining, while melanocytes in the skin were studied through melanin-specific Ferro-ferricyanide staining. The ultrastructure of the juvenile skin, hair follicles, and melanocytes was also explored. In both the juvenile and adult four-striped mouse, pigment-containing cells were observed in the dermis and were homogeneously dispersed throughout this layer. Apart from these cells, the histology of the skin of the adult four-striped mouse was similar to normal mammalian skin. In the juvenile four-striped mouse, abundant hair follicles of varying sizes were observed in the dermis and hypodermis, while hair follicles of similar size were only present in the dermis of adult four-striped mouse. Ultrastructural analysis of juvenile hair follicles revealed that the arrangement and differentiation of cellular layers were typical of a mammal. This study therefore provides unique transition pattern in the four-striped mouse skin morphology different from the textbook description of the normal mammalian skin.

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