The role of peripheral blood, bone marrow aspirate and especially bone marrow trephine biopsy in distinguishing atypical chronic myeloid leukemia from chronic granulocytic leukemia and chronic myelomonocytic leukemia

2009 ◽  
Vol 83 (4) ◽  
pp. 292-301 ◽  
Author(s):  
Gong Xubo ◽  
Lu Xingguo ◽  
Wu Xianguo ◽  
Xu Rongzhen ◽  
Xiao Xibin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Myeloid Leukemia ◽  
Bone Marrow Aspirate ◽  
Trephine Biopsy ◽  
Chronic Granulocytic Leukemia ◽  
Bone Marrow Trephine Biopsy ◽  
Atypical Chronic Myeloid Leukemia ◽  
Myelomonocytic Leukemia ◽  
Granulocytic Leukemia

scholarly journals Karyotypic polymorphism in acute myelofibrosis

Blood ◽  
1982 ◽  
Vol 60 (4) ◽  
pp. 841-844 ◽  
Author(s):  
I Shah ◽  
K Mayeda ◽  
F Koppitch ◽  
S Mahmood ◽  
B Nemitz
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Black Male ◽  
Blood Cells ◽  
Normal Karyotype ◽  
Trisomy 8 ◽  
Chronic Granulocytic Leukemia ◽  
Cytogenetic Abnormalities ◽  
Granulocytic Leukemia

Abstract Acute myelofibrosis (AMF) was diagnosed in a 59-yr-old black male in September 1978, on the basis of pancytopenia, lack of hepatosplenomegaly, fibrosis of the marrow, and paucity of teardrop red blood cells in the peripheral blood. Since then the patient has demonstrated an unusually long survival of 36 mo with a changing cytogenetic course. His initial 46, XY normal karyotype changed in 20 mo to trisomy 8, followed 1 yr later by 1:4 translocation in peripheral blood. Simultaneously with these changes, the fibrosis in the bone marrow progressively decreased, ultimately terminating in chronic granulocytic leukemia-like presentation with reversal to 46, XY karyotype. Fibroblast culture failed to show any evidence of cytogenetic abnormalities. The disappearance of fibrosis confirmed by trichrome and reticulin stains and lack of cytogenetic abnormalities in fibroblasts confirms the secondary role of fibrosis.

scholarly journals Karyotypic polymorphism in acute myelofibrosis

Blood ◽  
1982 ◽  
Vol 60 (4) ◽  
pp. 841-844
Author(s):  
I Shah ◽  
K Mayeda ◽  
F Koppitch ◽  
S Mahmood ◽  
B Nemitz
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Black Male ◽  
Blood Cells ◽  
Normal Karyotype ◽  
Trisomy 8 ◽  
Chronic Granulocytic Leukemia ◽  
Cytogenetic Abnormalities ◽  
Granulocytic Leukemia

Acute myelofibrosis (AMF) was diagnosed in a 59-yr-old black male in September 1978, on the basis of pancytopenia, lack of hepatosplenomegaly, fibrosis of the marrow, and paucity of teardrop red blood cells in the peripheral blood. Since then the patient has demonstrated an unusually long survival of 36 mo with a changing cytogenetic course. His initial 46, XY normal karyotype changed in 20 mo to trisomy 8, followed 1 yr later by 1:4 translocation in peripheral blood. Simultaneously with these changes, the fibrosis in the bone marrow progressively decreased, ultimately terminating in chronic granulocytic leukemia-like presentation with reversal to 46, XY karyotype. Fibroblast culture failed to show any evidence of cytogenetic abnormalities. The disappearance of fibrosis confirmed by trichrome and reticulin stains and lack of cytogenetic abnormalities in fibroblasts confirms the secondary role of fibrosis.

scholarly journals Bone Marrow Response to Erythropoietin in Polycythemia Vera and Chronic Granulocytic Leukemia

Blood ◽  
1972 ◽  
Vol 39 (3) ◽  
pp. 341-346 ◽  
Author(s):  
Stanley Zucker ◽  
Diane M. Howe ◽  
Lewis R. Weintraub
Keyword(s):  
Bone Marrow ◽  
Polycythemia Vera ◽  
Bone Marrow Cells ◽  
Normal Subjects ◽  
Normal Range ◽  
Chronic Granulocytic Leukemia ◽  
Iron Incorporation ◽  
Granulocytic Leukemia

Abstract The regulation of erythropoiesis was studied in patients with polycythemia vera and chronic granulocytic leukemia (CGL). An in vitro culture system was employed to determine the response of bone marrow cells to erythropoietin. In normal subjects, iron incorporation into heme (during the 18th to 22nd hr of culture) was increased by 35% (mean) in erythropoietin-treated cultures as compared to control cultures. Erythropoietin was ineffective in stimulating iron incorporation into heme in five patients with polycythemia vera. The marrow response to erythropoietin in vitro was within the normal range in three patients with CGL (37% stimulation). Thus, the role of erythropoietin in the control of erythropoiesis in myeloproliferative syndromes appears variable.

scholarly journals Molecular diagnosis of Iraqi chronic myeloid leukemia patients using quantitative real-time PCR

2010 ◽  
Vol 4 (2) ◽  
pp. 64-69
Author(s):  
Adeeb Abbas ◽  
Majeed A. Sabbah ◽  
Abdul-salam Hatam ◽  
Luma A. Yasser ◽  
Baan Abdul-Latif
Keyword(s):  
Bone Marrow ◽  
Real Time ◽  
Real Time Pcr ◽  
Myeloid Leukemia ◽  
Rna Extraction ◽  
Fusion Transcript ◽  
Quantitative Real Time Pcr ◽  
Rt Pcr ◽  
Chronic Granulocytic Leukemia ◽  
Granulocytic Leukemia

hronic myeloid leukemia (CML), also known as chronic granulocytic leukemia, is a form of leukemia characterized by the increased and unregulated growth of myeloid cells in the bone marrow and the accumulation of these cells in peripheral blood. Total RNA extraction, cDNA and quantitative real-time PCR (Q-RT-PCR) were done for thirty CML Iraqi patients. Hematology tests (hemoglobin, platelets and WBCs counts) were done for the same samples in the same time. The results of this study show that some samples with normal hematology values have BCR-ABL (p210) fusion transcript with molecular analysis by Q-RT-PCR. This indicates the importance of this technique in the diagnosis and monitoring the therapy of CML patients.

Hematological profile in pyrexia of unknown origin: role of bone marrow trephine biopsy vis-à-vis aspiration

Hematology ◽  
2008 ◽  
Vol 13 (5) ◽  
pp. 307-312 ◽  
Author(s):  
Ruchika Gupta ◽  
Namrata Setia ◽  
Prerna Arora ◽  
Sompal Singh ◽  
Tejinder Singh
Keyword(s):  
Bone Marrow ◽  
Unknown Origin ◽  
Trephine Biopsy ◽  
Bone Marrow Trephine ◽  
Pyrexia Of Unknown Origin ◽  
Bone Marrow Trephine Biopsy ◽  
Hematological Profile

scholarly journals Chronic myelomonocytic leukemia “myelodysplastic type’’ in transformation to acute myeloid leukemia – diagnostic and therapeutic options: case report and literature review / Leucemie mielomonocitară cronică forma mielodisplazică în transformare spre leucemie acută mieloidă – diagnostic și opțiuni terapeutice: prezentare de caz și revizuirea literaturii

2016 ◽  
Vol 24 (3) ◽  
pp. 263-277
Author(s):  
Mihaela Cîrstea ◽  
Adriana Coliță ◽  
Bogdan Ionescu ◽  
Didona Vasilache ◽  
Camelia Dobrea ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Myeloid Leukemia ◽  
Stem Cell ◽  
Complete Remission ◽  
Myeloid Leukemia ◽  
Bone Marrow Aspirate ◽  
Chronic Myelomonocytic Leukemia ◽  
Hematopoietic Stem ◽  
Myelomonocytic Leukemia ◽  
Acute Myeloid

Abstract Chronic myelomonocytic leukemia (CMML) is a clonal hematopoietic stem cell disorder that is characterized by the presence of an absolute monocytosis (1 × 10^ 9/l) in the peripheral blood, the overlap of myelodisplastic aspects and myeloproliferative aspects in the bone marrow and tendency to transform into acute myeloid leukemia. CMML is considered to be the most aggressive chronic myeloid leukemia. We present the case of a 48 years old woman who was hospitalized in March 2013 in the Center of Hematology and Bone Marrow Transplantation for anemia related symptoms. Initial investigations showed anemia, relative monocytosis (10% monocytes of the WBC differential) with an increasing absolute number of monocytes (> 1,000/μl) in the following months. Initial exploration of the bone marrow (aspirate and bone marrow biopsy and immunohistochemistry IHC tests) revealed elements of trilinear dysplasia and an increased percentage of myeloblasts (11-14%). In the next four months myeloblasts percentage remained below 20% (8-14%) and it has been observed a gradually increasing of monocytoid elements (> 20%). Immunophenotyping in the bone marrow aspirate identified a monocytic proliferation with high percentage (8%) of immature cells. The karyotype reported the presence of clones with t (1;3). Initially diagnosed as RAEB-2 (WHO) the case was recomitted in CMML-type 2 with a progression to acute myeloid leukemia (AML). Allogeneic hematopoietic stem cell transplantation (allo-HSCT) has been performed after getting the best possible therapeutic response with AML chemotherapy type (complete remission). Allo-HSCT was performed using myeloablative conditioning, 12 months after diagnosis. The patient is now in complete remission, 24 months after allo-HSCT.

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