Hyperactive behaviour in the mouse model of mucopolysaccharidosis IIIB in the open field and home cage environments

2011 ◽  
Vol 10 (6) ◽  
pp. 673-682 ◽  
Author(s):  
A. Langford-Smith ◽  
M. Malinowska ◽  
K. J. Langford-Smith ◽  
G. Wegrzyn ◽  
S. Jones ◽  
...  
Keyword(s):  
Mouse Model ◽  
Open Field ◽  
Home Cage ◽  
Hyperactive Behaviour

Chemically Contaminated Eel Fed to Pregnant and Lactating Mouse Dams Causes Hyperactivity in Their Offspring

2016 ◽  
Vol 86 (1-2) ◽  
pp. 36-47 ◽  
Author(s):  
Imen Dridi ◽  
Nidhal Soualeh ◽  
Torsten Bohn ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Open Field ◽  
Early Life ◽  
Home Cage ◽  
Anguilla Anguilla ◽  
Acetylcholinesterase Activity ◽  
Female Offspring ◽  
Significant Inhibition ◽  
Standard Diet ◽  
Early Life Exposure ◽  
Cage Test

Abstract.This study examined whether perinatal exposure to polluted eels (Anguilla anguilla L.) induces changes in the locomotor activity of offspring mice across lifespan (post-natal days (PNDs) 47 – 329), using the open field and the home cage activity tests. Dams were exposed during gestation and lactation, through diets enriched in eels naturally contaminated with pollutants including PCBs. Analysis of the eel muscle focused on the six non-dioxin-like (NDL) indicator PCBs (Σ6 NDL-PCBs: 28, 52, 101, 138, 153 and 180). Four groups of dams (n = 10 per group) received either a standard diet without eels or eels (0.8 mg/kg/day) containing 85, 216, or 400 ng/kg/day of ϵ6 NDL-PCBs. The open field test showed that early-life exposure to polluted eels increased locomotion in female offspring of exposed dams but not in males, compared to controls. This hyperlocomotion appeared later in life, at PNDs 195 and 329 (up to 32 % increase, p < 0.05). In addition, overactivity was observed in the home cage test at PND 305: exposed offspring females showed a faster overall locomotion speed (3.6 – 4.2 cm/s) than controls (2.9 cm/s, p <0.05); again, males remained unaffected. Covered distances in the home cage test were only elevated significantly in offspring females exposed to highest PCB concentrations (3411 ± 590 cm vs. 1377 ± 114 cm, p < 0.001). These results suggest that early-life exposure to polluted eels containing dietary contaminants including PCBs caused late, persistent and gender-dependent neurobehavioral hyperactive effects in offspring mice. Furthermore, female hyperactivity was associated with a significant inhibition of acetylcholinesterase activity in the hippocampus and the prefrontal cortex.

Screening Approaches to Neurotoxicity: A Functional Observational Battery

1989 ◽  
Vol 8 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Virginia Clayton Moser
Keyword(s):  
Open Field ◽  
Home Cage ◽  
Profile Analysis ◽  
Research Effort ◽  
Field Observations ◽  
Laboratory Rats ◽  
Primary Screening ◽  
Specificity And Sensitivity ◽  
Screening Approaches ◽  
Functional Observational Battery

Observational tests to assess the behavioral and neurologic integrity of laboratory rats have been advanced for use in the primary screening of chemicals to detect neurotoxic potential. To examine the use of such an approach, we are systematically using a functional observational battery (FOB) to characterize known neurotoxicants and to assess its selectivity, reliability, specificity, and sensitivity. Our FOB is a series of tests that includes home cage and open field observations, neuromuscular and sensorimotor tests, and physiologic measures. Described in this article are the compounds we have tested to date and how the data have been integrated to produce a profile of effects for each. We have found the FOB to be sensitive to a variety of neurotoxicants, whose effects can be distinguished using a type of profile analysis as illustrated herein. This research effort provides important information about both the execution and the interpretation of FOB studies.

scholarly journals Who's the Boss? Assessing Convergent Validity of Aggression Based Dominance Measures in Male Laboratory Mice, Mus Musculus

2021 ◽  
Vol 8 ◽  
Author(s):  
Amanda J. Barabas ◽  
Jeffrey R. Lucas ◽  
Marisa A. Erasmus ◽  
Heng-Wei Cheng ◽  
Brianna N. Gaskill
Keyword(s):  
Open Field ◽  
Test Scores ◽  
Convergent Validity ◽  
Discriminant Validity ◽  
Home Cage ◽  
Dominance Rank ◽  
Mitigation Strategies ◽  
Male Mice ◽  
Social Investigation ◽  
Tube Test

Aggression among group housed male mice continues to challenge laboratory animal researchers because mitigation strategies are generally applied at the cage level without a good understanding of how it affects the dominance hierarchy. Aggression within a group is typically displayed by the dominant mouse targeting lower ranking subordinates; thus, the strategies for preventing aggression may be more successful if applied specifically to the dominant mouse. Unfortunately, dominance rank is often not assessed because of time intensive observations or tests. Several correlates of dominance status have been identified, but none have been directly compared to home cage behavior in standard housing. This study assessed the convergent validity of three dominance correlates (urinary darcin, tube test score, preputial gland to body length ratio) with wound severity and rankings based on home cage behavior, using factor analysis. Discriminant validity with open field measures was assessed to determine if tube test scores are independent of anxiety. Cages were equally split between SJL and albino C57BL/6 strains and group sizes of 3 or 5 (N = 24). Home cage behavior was observed during the first week, and dominance measures were recorded over the second. After controlling for strain and group size, darcin and preputial ratio had strong loadings on the same factor, which was a significant predictor of home cage ranking showing strong convergent validity. Tube test scores were not significantly impacted by open field data, showing discriminant validity. Social network analysis revealed that despotic power structures were prevalent, aggressors were typically more active and rested away from cage mates, and the amount of social investigation and aggression performed by an individual were highly correlated. Data from this study show that darcin and preputial ratio are representative of home cage aggression and provide further insight into individual behavior patterns in group housed male mice.

scholarly journals Spatial coding defects of hippocampal neural ensemble calcium activities in the 3xTg-AD Alzheimer’s disease mouse model

2021 ◽  
Author(s):  
Xiaoxiao Lin ◽  
Lujia Chen ◽  
David Baglietto-Vargas ◽  
Qiao Ye ◽  
Frank M LaFerla ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Open Field ◽  
Neural Population ◽  
Spatial Coding ◽  
Calcium Activity ◽  
Age Related ◽  
Neural Ensemble

Abstract Background Alzheimer’s disease (AD) causes progressive age-related defects in memory and cognitive function, and has emerged as a major health and socio-economic concern in the US and worldwide. To develop effective therapeutic treatments for AD, we need to better understand the neural mechanisms by which AD causes memory loss and cognitive deficits. Methods Here we examine large scale hippocampal neural population calcium activities imaged at single cell resolution in a triple-transgenic Alzheimer’s disease mouse model (3xTg-AD) that presents both amyloid plaque and neurofibrillary pathological features along with age-related behavioral defects. To measure encoding of environmental location in hippocampal neural ensembles in the 3xTg-AD mice in vivo, we performed GCaMP6-based calcium imaging using head-mounted, miniature fluorescent microscopes (“miniscopes”) on freely moving animals. We compared hippocampal CA1 excitatory neural ensemble activities during open-field exploration and track-based route-running behaviors in age-matched AD and control mice at young (3–6.5 months old) and old (18–21 months old) animals. Results 3xTg-AD CA1 excitatory cells display significantly higher calcium activity rates compared with Non-Tg controls for both the young and old age groups during spatial exploration, suggesting that in vivo enhanced neuronal calcium ensemble activity is a disease feature. Increased ages are correlated with decreased neural calcium activity rates across genotypes. CA1 neuronal populations of 3xTg-AD mice show lower spatial information scores compared with control mice. Spatial firing of CA1 neurons of old 3xTg-AD mice also displays higher sparsity and spatial coherence, indicating less place specificity for spatial representation. We find locomotion significantly modulates the amplitude of hippocampal neural calcium ensemble activities in 3xTg-AD mice, but not in non-transgenic controls during open field ambulatory movements. Conclusions Our data offers new and comprehensive information about age-dependent neural circuit activity changes in this important AD mouse model, and provides strong evidence that spatial coding defects in the neuronal population activities are associated with AD pathology and AD-related memory behavioral deficits.

scholarly journals An Automated Home-Cage System to Assess Learning and Performance of a Skilled Motor Task in a Mouse Model of Huntington’s Disease

eNeuro ◽  
2017 ◽  
Vol 4 (5) ◽  
pp. ENEURO.0141-17.2017 ◽  
Author(s):  
Cameron L. Woodard ◽  
Federico Bolaños ◽  
James D. Boyd ◽  
Gergely Silasi ◽  
Timothy H. Murphy ◽  
...  
Keyword(s):  
Mouse Model ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Home Cage ◽  
Motor Task ◽  
Cage System ◽  
And Performance ◽  
Learning And Performance

scholarly journals Behavioral and Pharmacological Evaluation of a Selectively Bred Mouse Model of Home Cage Hyperactivity

2014 ◽  
Vol 44 (5) ◽  
pp. 516-534 ◽  
Author(s):  
Petra Majdak ◽  
Paula J. Bucko ◽  
Ashley L. Holloway ◽  
Tushar K. Bhattacharya ◽  
Erin K. DeYoung ◽  
...  
Keyword(s):  
Mouse Model ◽  
Home Cage ◽  
Pharmacological Evaluation

scholarly journals Abnormalities of social interactions and home-cage behavior in a mouse model of Rett syndrome

2004 ◽  
Vol 14 (2) ◽  
pp. 205-220 ◽  
Author(s):  
Paolo Moretti ◽  
J. Adriaan Bouwknecht ◽  
Ryan Teague ◽  
Richard Paylor ◽  
Huda Y. Zoghbi
Keyword(s):  
Mouse Model ◽  
Social Interactions ◽  
Rett Syndrome ◽  
Home Cage

scholarly journals Estrogen Increases Locomotor Activity in Mice through Estrogen Receptor α: Specificity for the Type of Activity

Endocrinology ◽  
2003 ◽  
Vol 144 (1) ◽  
pp. 230-239 ◽  
Author(s):  
Sonoko Ogawa ◽  
Johnny Chan ◽  
Jan-Åke Gustafsson ◽  
Kenneth S. Korach ◽  
Donald W. Pfaff
Keyword(s):  
Locomotor Activity ◽  
Open Field ◽  
Home Cage ◽  
Placebo Control ◽  
Male Mice ◽  
Open Field Activity ◽  
Running Wheel ◽  
Field Activity ◽  
Wheel Activity ◽  
Estrogenic Regulation

Abstract Estrogens are known to increase running wheel activity of rodents primarily by acting on the medial preoptic area (mPOA). The mechanisms of this estrogenic regulation of running wheel activity are not completely understood. In particular, little is known about the separate roles of two types of estrogen receptors, ERα and ERβ, both of which are expressed in mPOA neurons. In the present study the effects of continuous estrogen treatment on running wheel activity were examined in male and female mice specifically lacking either the ERα (αERKO) or the ERβ (βERKO) gene. Mice were gonadectomized and 1 wk later implanted with either a low dose (16 ng/d) or a high dose (160 ng/d) of estradiol benzoate (EB) or with a placebo control pellet. Home cage running wheel activity was recorded for 9 d starting 10 d after EB implants. The same mice were also tested for open field activity before and after EB implants. In both female and male αERKO mice, running wheel activity was not different from that in corresponding wild-type (αWT) mice in placebo control groups. In both females and males it was increased by EB only in αWT, not αERKO, mice. In βERKO mice, on the other hand, both doses of EB equally increased running wheel activity in both sexes just as they did in βWT mice. Absolute numbers of daily revolutions of EB-treated groups, however, were significantly lower in βERKO females compared with βWT females. Before EB treatment, gonadectomized αERKO female were significantly less active than αWT mice in open field tests, whereas βERKO females tended to be more active than βWT mice. In male mice there were no effect of ERα or ERβ gene knockout on open field activity. Unlike its effect on running wheel activity, EB treatment induced only a small increase in open field activity in female, but not male, mice. These findings indicate that 1) in both sexes estrogenic regulation of running wheel activity is primarily mediated through the ERα, not the ERβ; and 2) hormone/genotype effects are specific to the type of locomotor activity (i.e. home cage running wheel activity and open field activity) measured.

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