Some observations made by intracellular recordings in a primary astrocyte culture and a glioblastoma ‘138 MG’

1986 ◽  
Vol 126 (3) ◽  
pp. 413-417 ◽  
Author(s):  
ÅKE NILSSON ◽  
EVA LIDÉN ◽  
ÅKE SELLSTRÖM
Keyword(s):  
Intracellular Recordings ◽  
Astrocyte Culture ◽  
Primary Astrocyte ◽  
Primary Astrocyte Culture

Glutamate Increases Glycogen Content and Reduces Glucose Utilization in Primary Astrocyte Culture

1990 ◽  
Vol 54 (2) ◽  
pp. 490-496 ◽  
Author(s):  
Raymond A. Swanson ◽  
Albert C. H. Yu ◽  
Pak H. Chan ◽  
Frank R. Sharp
Keyword(s):  
Glucose Utilization ◽  
Glycogen Content ◽  
Astrocyte Culture ◽  
Primary Astrocyte ◽  
Primary Astrocyte Culture

Establishment and characterization of primary astrocyte culture from adult mouse brain

2017 ◽  
Vol 132 ◽  
pp. 10-19 ◽  
Author(s):  
Xiu Sun ◽  
Xin Hu ◽  
Dan Wang ◽  
Yimin Yuan ◽  
Shangyao Qin ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Adult Mouse ◽  
Adult Mouse Brain ◽  
Astrocyte Culture ◽  
Primary Astrocyte ◽  
Primary Astrocyte Culture

scholarly journals A novel serum free primary astrocyte culture method that mimic quiescent astrocyte phenotype

2019 ◽  
Vol 320 ◽  
pp. 50-63 ◽  
Author(s):  
Jude Prah ◽  
Ali Winters ◽  
Kiran Chaudhari ◽  
Jessica Hersh ◽  
Ran Liu ◽  
...  
Keyword(s):  
Culture Method ◽  
Serum Free ◽  
Astrocyte Culture ◽  
Primary Astrocyte ◽  
Primary Astrocyte Culture

scholarly journals Effects of MRP8, LPS, and Lenalidomide on the Expressions of TNF-α, Brain-Enriched, and Inflammation-Related MicroRNAs in the Primary Astrocyte Culture

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Ahmed Omran ◽  
Muhammad Usman Ashhab ◽  
Na Gan ◽  
Huimin Kong ◽  
Jing Peng ◽  
...  
Keyword(s):  
Therapeutic Interventions ◽  
Necrosis Factor Alpha ◽  
Small Noncoding Rnas ◽  
Primary Astrocyte ◽  
Tnf Α ◽  
Factor Alpha ◽  
Novel Target ◽  
First Time ◽  
Primary Astrocyte Culture ◽  
Necrosis Factor

Astrocytes are now recognized as a heterogeneous class of cells with many important and diverse functions in healthy and diseased central nervous system (CNS). MicroRNAs (miRNAs) are small, noncoding RNAs which may have key roles in astrocytes activation in response to various stimuli. We performed quantitative real-time PCR (qPCR) to detect changes in the expressions of brain-enriched miRNAs (124, 134, 9, 132, and 138), inflammation-related miRNAs (146a, 21, 181a, 221, and 222), and tumor necrosis factor alpha (TNF-α) in the rat primary astrocyte cultures after stimulation with myeloid-related protein 8 (MRP8) and lipopolysaccharides (LPS). Further, we inhibited the expression of TNF-αin the astrocytes by using TNF-αinhibitor (lenalidomide) and tested for the first time the effect of this inhibition on the expressions of the same tested miRNAs. Stimulation of the astrocytes with MRP8 or LPS leads to significant upregulation of miRNAs (124, 134, 9, 132, 146a, 21, 181a, 221, and 222), while miRNA-138 was downregulated. TNF-αinhibition with lenalidomide leads to opposite expressions of the tested miRNAs. These miRNAs may play an important role in activation of the astrocytes and may be a novel target for cell-specific therapeutic interventions in multiple CNS diseases.

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