Expression of LDL Receptor Binding Determinants in Very Low Density Lipoproteinsa

1985 ◽  
Vol 454 (1) ◽  
pp. 239-247 ◽  
Author(s):  
WILLIAM A. BRADLEY ◽  
ANTONIO M. GOTTO ◽  
SANDRA H. GIANTURCO
Keyword(s):  
Receptor Binding ◽  
Ldl Receptor ◽  
Low Density ◽  
Binding Determinants

Increase in In Vivo Low-Density Lipoprotein (LDL) Receptor Binding after PGE1 and 13,14-Dihydro-PGE1 Treatment in Rabbits

1993 ◽  
Vol 21 (3) ◽  
pp. 503-506 ◽  
Author(s):  
H. Sinzinger ◽  
Irene Virgolini ◽  
S. R. Li ◽  
A. Gerakakis ◽  
P. Fitscha ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Low Density Lipoprotein ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Low Density

scholarly journals Interaction of 4-hydroxynonenal-modified low-density lipoproteins with the fibroblast apolipoprotein B/E receptor

1986 ◽  
Vol 234 (1) ◽  
pp. 245-248 ◽  
Author(s):  
W Jessup ◽  
G Jurgens ◽  
J Lang ◽  
H Esterbauer ◽  
R T Dean
Keyword(s):  
Apolipoprotein B ◽  
Negative Charge ◽  
Low Density Lipoprotein ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipid Peroxidation Product ◽  
Modified Low Density Lipoproteins ◽  
Peroxidation Product

The incorporation of the lipid peroxidation product 4-hydroxynonenal into low-density lipoprotein (LDL) increases the negative charge of the particle, and decreases its affinity for the fibroblast LDL receptor. It is suggested that this modification may occur in vivo, and might promote atherogenesis.

Transporting mitochondrion-targeting photosensitizers into cancer cells by low-density lipoproteins for fluorescence-feedback photodynamic therapy

Nanoscale ◽  
2021 ◽  
Author(s):  
Chao Wang ◽  
Xianhao Zhao ◽  
Haoyu Jiang ◽  
Jiaxin Wang ◽  
Weixiu Zhong ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Ldl Receptor ◽  
Low Density Lipoproteins ◽  
Low Density

Low-density lipoproteins (LDLs) reconstituted with a multifunctional mitochondrion-targeting photosensitizer are able to achieve fluorescence-feedback photodynamic therapy of LDL receptor-overexpressing cancer cells.

scholarly journals Detection of the low-density-lipoprotein receptor with biotin-low-density lipoprotein. A rapid new method for ligand blotting

1985 ◽  
Vol 229 (3) ◽  
pp. 785-790 ◽  
Author(s):  
D P Wade ◽  
B L Knight ◽  
A K Soutar
Keyword(s):  
Low Density Lipoprotein ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
High Sensitivity ◽  
Receptor Protein ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Cell Extracts ◽  
Density Lipoprotein Receptor ◽  
A New Technique

A new technique has been developed to identify low-density-lipoprotein (LDL) receptors on nitrocellulose membranes, after transfer from SDS/polyacrylamide gels, by ligand blotting with biotin-modified LDL. Modification with biotin hydrazide of periodate-oxidized lipoprotein sugar residues does not affect the ability of the lipoprotein to bind to the LDL receptor. Bound lipoprotein is detected with high sensitivity by a streptavidin-biotin-peroxidase complex, and thus this method eliminates the need for specific antibodies directed against the ligand. The density of the bands obtained is proportional to the amount of pure LDL receptor protein applied to the SDS/polyacrylamide gel, so that it is possible to quantify LDL receptor protein in cell extracts. Biotin can be attached to other lipoproteins, for example very-low-density lipoproteins with beta-mobility, and thus the method will be useful in the identification and isolation of other lipoprotein receptors.

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