Topical application of autologous adipose‐derived mesenchymal stem cells (AdMSCs) for persistent sterile corneal epithelial defect

Purpose: To present a series of cases of corneal chemical burns associated with the use of likely improperly buffered corneal lubricant gel, Goniovisc, during uncomplicated, posterior segment surgery. Methods: Cases of postoperative corneal complications following surgeries in which Goniovisc was used were collected from 6 retina practices across the United States between January and August 2016. The preoperative diagnosis, type of procedure, postoperative day 1 corneal findings, treatment method, and time to resolution of the corneal irregularity were recorded. pH testing of Goniovisc lots from this time period was performed using Hydrion litmus paper. Results: A total of 157 eyes of 156 patients were found to have corneal complications following relatively simple posterior segment surgeries in which Goniovisc was used. Findings included one or more of the following: corneal epithelial defect (84% of cases), Descemet folds (34%), stromal edema (14%), and superficial punctate keratitis (3%). Time to resolution ranged from 2 to 90 days (mean, 12 days; median, 7 days). Treatment methods included artificial tears (37%), bandage contact lens (28%), antibiotic drops (17%), antibiotic ointment (16%), and patching (9%). Two cases were treated with amniotic membrane grafts. One case required a penetrating keratoplasty due to an old laser-assisted in situ keratomileusis (LASIK) flap with a nonhealing epithelial defect that scarred significantly. Litmus testing of Goniovisc lots from this time period revealed a pH of 4 to 5. Conclusion: Chemical burns secondary to improperly buffered corneal lubricant solution should be considered in the differential when corneal injury is encountered following posterior segment surgery.

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Case report: persistent corneal epithelial defect after glaucoma device implantation for radiation glacoma

Acta Ophthalmologica ◽

10.1111/j.1755-3768.2019.5323 ◽

2019 ◽

Vol 97 (S263) ◽

Author(s):

Daisuke Shimizu ◽

Ayako Tawada ◽

Shuichi Yamamoto

Keyword(s):

Case Report ◽

Epithelial Defect ◽

Corneal Epithelial ◽

Corneal Epithelial Defect ◽

Device Implantation

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Subconjunctival Injection of Transdifferentiated Oral Mucosal Epithelial Cells for Limbal Stem Cell Deficiency in Rats

Journal of Histochemistry & Cytochemistry ◽

10.1369/0022155420980071 ◽

2020 ◽

pp. 002215542098007

Author(s):

Yu-Ting Xiao ◽

Hua-Tao Xie ◽

Xin Liu ◽

Chao-Ye Duan ◽

Jing-Yu Qu ◽

...

Keyword(s):

Stem Cell ◽

Epithelial Cells ◽

Pigment Epithelium ◽

Limbal Stem Cell Deficiency ◽

Subconjunctival Injection ◽

Epithelial Defect ◽

Corneal Epithelial ◽

Corneal Epithelial Defect ◽

Limbal Stem Cell

Rat limbal niche cells (LNCs) have been proven to induce transdifferentiation of oral mucosal epithelial cells (OMECs) into corneal epithelial-like cells termed transdifferentiated oral mucosal epithelial cells (T-OMECs). This investigation aimed to evaluate the effect of subconjunctival T-OMEC injections on alkali-induced limbal stem cell deficiency (LSCD) in rats. LNCs were cocultured with OMECs in the Transwell system to obtain T-OMECs, with NIH-3T3 cells serving as a control. Subconjunctival injection of single T-OMEC or OMEC suspension was performed immediately after corneal alkali injury. T-OMECs were prelabeled with the fluorescent dye CM-DiI in vitro and tracked in vivo. Corneal epithelial defect, opacity, and neovascularization were quantitatively analyzed. The degree of corneal epithelial defect (from day 1 onward), opacity (from day 5 onward), and neovascularization (from day 2 onward) was significantly less in the T-OMEC group than in the OMEC group. Cytokeratin 12 (CK12), pigment epithelium–derived factor, and soluble fms-like tyrosine kinase-1 were expressed at a higher rate following T-OMEC injection. Some CM-DiI-labeled cells were found to be coexpressed with CK12, Pax6, and ΔNp63α in the corneal epithelium after subconjunctival injection. Subconjunctival injection of T-OMECs prevents conjunctival invasion and maintains a normal corneal phenotype, which might be a novel strategy in the treatment of LSCD:

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Combined eye gel containing sodium hyaluronate and xanthan gum for the treatment of the corneal epithelial defect after pterygium surgery

Clinical Ophthalmology ◽

10.2147/opth.s85638 ◽

2015 ◽

pp. 1463 ◽

Cited By ~ 1

Author(s):

Tolga Kocaturk ◽

Ataman Gencgonul ◽

Faruk Balica ◽

Harun Cakmak ◽

Mehmet Ozbagcivan

Keyword(s):

Xanthan Gum ◽

Sodium Hyaluronate ◽

Epithelial Defect ◽

Corneal Epithelial ◽

Corneal Epithelial Defect ◽

Pterygium Surgery

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Outcome of Amniotic Membrane Transplant in Persistent Corneal Epithelial Defects

Pakistan Journal of Ophthalmology ◽

10.36351/pjo.v38i1.1340 ◽

2021 ◽

Vol 38 (1) ◽

Author(s):

Tiabbah Saleem ◽

Syeda Ayesha Bokhari

Keyword(s):

Amniotic Membrane ◽

Case Series ◽

Bullous Keratopathy ◽

Epithelial Defect ◽

Corneal Epithelial ◽

Corneal Epithelial Defect ◽

Case Series Study ◽

Chemical Injuries ◽

Standard Medical Treatment ◽

Disease Study

Purpose: To determine the outcome of amniotic membrane transplant in patients with ocular surface disease. Study Design: Interventional Case series study Place and Duration of Study: Layton Rahmatullah Benevolent Trust Eye Hospital, Korangi 2½, Karachi, from January, 2019 to July 2019. Methods: Patients with an age range of 20–45 years, either gender and who had persistent corneal epithelial defects as a consequence of keratitis, chemical injuries, bullous keratopathy and Mooren’s ulcers were included. Informed consent was obtained. Preserved amniotic membrane was used in all patients and they were followed at 1 week, 1, 3 and finally at 6 months and results were evaluated in terms of stable, healed and pain-free cornea. Results: Mean age of the patients was 37.94 ± 6.78 years. Majority of the patients (60.0%) were less than 40 years of age. Out of 58 patients, 45 (77.6%) were males and 13 (22.4%) were females. Mean duration of injury was 7.67 ± 2.28 weeks with majority of patients i.e. 62% were with less than 8 weeks of duration. Most common cause of persistent corneal epithelial defect was bacterial keratitis (17.2%) followed by vegetative trauma (13.8%). Successful surgical outcome as healed cornea was observed in 53 (91.4%) patients. Conclusion: Amniotic membrane transplant is an effective treatment for persistent corneal epithelial defects unresponsive to standard medical treatment. This surgical technique is effective in terms of healing and resolving the inflammation and preserving the useful vision. Key Words: Persistant corneal epithelial defect, Amniotic Membrane, Bullous keratopathy.

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Topical application of adipose tissue-derived mesenchymal stem cells (ADMSCs) reduced cerebral edema in experimental traumatic brain injury (TBI)—a preliminary study

Chinese Neurosurgical Journal ◽

10.1186/s41016-020-00219-9 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Hui Ma ◽

Lian Xu Cui ◽

Ping Kuen Lam ◽

Cindy S. W. Tong ◽

Kin K. Y. Lo ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Brain Injury ◽

Topical Application ◽

Cerebral Edema ◽

Therapeutic Effects ◽

Edema Formation ◽

Hypoxic Precondition ◽

Preliminary Study

Abstract Background Our previous studies showed that topical application of mesenchymal stem cells (MSCs) improved functional recovery in rat traumatic brain injury (TBI) model, and hypoxic precondition further enhanced the therapeutic effects of MSCs. There was no previous study on the attenuation of cerebral edema by MSCs. We investigated whether topical application of normoxic and hypoxic MSCs could reduce cerebral edema in an experimental TBI model. Methods Two million normoxic (N = 24) and hypoxic (N = 24) MSCs were applied topically to exposed the cerebral cortex in a controlled cortical impact (CCI) model. The MSCs were fixed in position with fibrin glue. No treatment was given to control animals (TBI only: n = 24). After surgery, four animals in each group were sacrificed daily (day 1 to day 6) for edema evaluation. Normal animals without TBI were used as reference (n = 4). The expressions of GFAP, AQP4, and MMP9 were also investigated by immunofluorescence staining and RT-PCR at day 3. Results The edema peaked within 3 days after TBI. Compared with the control, hypoxic MSCs reduced brain water content significantly (p < 0.05). Both hypoxic and normoxic MSCs downregulated the expression of MMP9 and normalized AQP4 distribution to astrocyte end feet. Conclusion Our preliminary study showed that topical application of hypoxic MSCs suppressed both vasogenic and cytotoxic edema formation.

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