Bactericidal effects of a high-power, red light-emitting diode on two periodontopathic bacteria in antimicrobial photodynamic therapy in vitro

Photodynamic therapy (PDT) has been commonly used in treating many diseases, such as cancer and infectious diseases. We investigated the different effects of PDT on three main pathogenic bacteria of periodontitis — Prevotella melaninogenica (P.m.), Porphyromonas gingivalis (P.g.) and Aggregatibacter actinomycetemcomitans (A.a.). The portable red light-emitting diode (LED) phototherapy device was used to assess the exogenous PDT effects with different light doses and photosensitizer concentrations (Toluidine blue O, TBO). The portable blue LED phototherapy device was used to assess the endogenous PDT effects with the use of endogenous photosensitizers (porphyrin) under different light doses. We found out that both exogenous and endogenous PDT were able to restrict the growth of all the three bacteria significantly. Moreover, the optimal PDT conditions for these bacteria were obtained through this in vitro screening and could guide the clinical PDT on periodontitis.

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Organic Light Emitting Diode for in vitro Antimicrobial Photodynamic Therapy of Candida Strains

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2021.102567 ◽

2021 ◽

pp. 102567

Author(s):

Uriel Melendez-Celis ◽

Teresita Spezzia-Mazzocco ◽

Saydulla Persheyev ◽

Cheng Lian ◽

Ifor Samuel ◽

...

Keyword(s):

Photodynamic Therapy ◽

Light Emitting Diode ◽

Antimicrobial Photodynamic Therapy ◽

Light Emitting ◽

Organic Light Emitting Diode ◽

Organic Light

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Faculty Opinions recommendation of Topical methyl aminolevulinate photodynamic therapy using red light-emitting diode light for multiple actinic keratoses: a randomized study.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1160996.621442 ◽

2009 ◽

Author(s):

Juerg Hafner

Keyword(s):

Photodynamic Therapy ◽

Light Emitting Diode ◽

Randomized Study ◽

Red Light ◽

Light Emitting ◽

Actinic Keratoses ◽

Methyl Aminolevulinate

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In Vitro Effect of Photodynamic Therapy with Different Lights and Combined or Uncombined with Chlorhexidine on Candida spp.

Pharmaceutics ◽

10.3390/pharmaceutics13081176 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1176

Author(s):

Vanesa Pérez-Laguna ◽

Yolanda Barrena-López ◽

Yolanda Gilaberte ◽

Antonio Rezusta

Keyword(s):

Photodynamic Therapy ◽

Light Emitting Diode ◽

Antimicrobial Effect ◽

Photodynamic Treatment ◽

Candida Spp ◽

Promising Alternative ◽

Light Emitting ◽

Colony Forming Units ◽

Vitro Effect

Candidiasis is very common and complicated to treat in some cases due to increased resistance to antifungals. Antimicrobial photodynamic therapy (aPDT) is a promising alternative treatment. It is based on the principle that light of a specific wavelength activates a photosensitizer molecule resulting in the generation of reactive oxygen species that are able to kill pathogens. The aim here is the in vitro photoinactivation of three strains of Candida spp., Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258, using aPDT with different sources of irradiation and the photosensitizer methylene blue (MB), alone or in combination with chlorhexidine (CHX). Irradiation was carried out at a fluence of 18 J/cm2 with a light-emitting diode (LED) lamp emitting in red (625 nm) or a white metal halide lamp (WMH) that emits at broad-spectrum white light (420–700 nm). After the photodynamic treatment, the antimicrobial effect is evaluated by counting colony forming units (CFU). MB-aPDT produces a 6 log10 reduction in the number of CFU/100 μL of Candida spp., and the combination with CHX enhances the effect of photoinactivation (effect achieved with lower concentration of MB). Both lamps have similar efficiencies, but the WMH lamp is slightly more efficient. This work opens the doors to a possible clinical application of the combination for resistant or persistent forms of Candida infections.

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ERYTHROSINE AS A PHOTOSENSITIZER FOR ANTIMICROBIAL PHOTODYNAMIC THERAPY WITH BLUE LIGHT-EMITTING DIODES – AN IN VITRO STUDY

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2021.102445 ◽

2021 ◽

pp. 102445

Author(s):

Marcela Leticia Leal Gonçalves ◽

Elaine Marcílio Santos ◽

Ana Cláudia Muniz Renno ◽

Anna Carolina Ratto Tempestini Horliana ◽

Matheus de Almeida Cruz ◽

...

Keyword(s):

Photodynamic Therapy ◽

Blue Light ◽

Light Emitting Diodes ◽

In Vitro Study ◽

Vitro Study ◽

Antimicrobial Photodynamic Therapy ◽

Light Emitting

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Antimicrobial effect of photodynamic therapy using sinoporphyrin sodium and 390–400 nm light-emitting diode on Porphyromonas gingivalis in vitro

Lasers in Medical Science ◽

10.1007/s10103-020-03067-2 ◽

2020 ◽

Vol 36 (1) ◽

pp. 153-164

Author(s):

Yuqi Song ◽

Jiang Lin ◽

Zhiguo Zhang ◽

Bin Xu ◽

Liangjia Bi

Keyword(s):

Photodynamic Therapy ◽

Porphyromonas Gingivalis ◽

Light Emitting Diode ◽

Antimicrobial Effect ◽

Light Emitting

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IN VITRO DEVELOPMENT OF YELLOW LAPACHO (BIGNONIACEAE) USING HIGH-POWER LIGHT EMITTING DIODE

Revista Árvore ◽

10.1590/1806-90882018000500008 ◽

2018 ◽

Vol 42 (5) ◽

Author(s):

Ezequiel Enrique Larraburu ◽

Gonzalo Sanchez Correa ◽

Berta Elizabet Llorente

Keyword(s):

In Vitro Culture ◽

High Power ◽

Stomatal Density ◽

Light Emitting Diode ◽

Dry Weight ◽

Light Sources ◽

Multiplication Rate ◽

Light Emitting ◽

Shoot Dry Weight

ABSTRACT Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.

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