Antimicrobial effect of photodynamic therapy using sinoporphyrin sodium and 390–400 nm light-emitting diode on Porphyromonas gingivalis in vitro

2020 ◽  
Vol 36 (1) ◽  
pp. 153-164
Author(s):  
Yuqi Song ◽  
Jiang Lin ◽  
Zhiguo Zhang ◽  
Bin Xu ◽  
Liangjia Bi
Keyword(s):  
Photodynamic Therapy ◽  
Porphyromonas Gingivalis ◽  
Light Emitting Diode ◽  
Antimicrobial Effect ◽  
Light Emitting

scholarly journals In Vitro Effect of Photodynamic Therapy with Different Lights and Combined or Uncombined with Chlorhexidine on Candida spp.

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1176
Author(s):  
Vanesa Pérez-Laguna ◽  
Yolanda Barrena-López ◽  
Yolanda Gilaberte ◽  
Antonio Rezusta
Keyword(s):  
Photodynamic Therapy ◽  
Light Emitting Diode ◽  
Antimicrobial Effect ◽  
Photodynamic Treatment ◽  
Candida Spp ◽  
Promising Alternative ◽  
Light Emitting ◽  
Colony Forming Units ◽  
Vitro Effect

Candidiasis is very common and complicated to treat in some cases due to increased resistance to antifungals. Antimicrobial photodynamic therapy (aPDT) is a promising alternative treatment. It is based on the principle that light of a specific wavelength activates a photosensitizer molecule resulting in the generation of reactive oxygen species that are able to kill pathogens. The aim here is the in vitro photoinactivation of three strains of Candida spp., Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258, using aPDT with different sources of irradiation and the photosensitizer methylene blue (MB), alone or in combination with chlorhexidine (CHX). Irradiation was carried out at a fluence of 18 J/cm2 with a light-emitting diode (LED) lamp emitting in red (625 nm) or a white metal halide lamp (WMH) that emits at broad-spectrum white light (420–700 nm). After the photodynamic treatment, the antimicrobial effect is evaluated by counting colony forming units (CFU). MB-aPDT produces a 6 log10 reduction in the number of CFU/100 μL of Candida spp., and the combination with CHX enhances the effect of photoinactivation (effect achieved with lower concentration of MB). Both lamps have similar efficiencies, but the WMH lamp is slightly more efficient. This work opens the doors to a possible clinical application of the combination for resistant or persistent forms of Candida infections.

scholarly journals Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study

2014 ◽  
Vol 08 (04) ◽  
pp. 509-514 ◽  
Author(s):  
Liliana Guimarães Oliveira Ricatto ◽  
Luis Augusto Lopato Conrado ◽  
Cecilia Pedroso Turssi ◽  
Fabiana Mantovani Gomes França ◽  
Roberta Tarkany Basting ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Light Emitting Diode ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Dye Laser ◽  
Laser Application ◽  
Light Emitting ◽  
Cariogenic Bacteria

ABSTRACT Objective: The aim of this study was to evaluate in vitro the effect of photodynamic therapy (PDT) using LASER or light emitting diode (LED) on cariogenic bacteria (Streptococcus mutans [SM] and Lactobacillus casei [LC]) in bovine dentin. Materials and Methods: Twenty five fragments of dentin were contaminated with SM and LC strands and divided into five experimental groups according to the therapy they received (n = 5): C – control (no treatment), SCLED – no dye/LED application (94 J/cm2), SCLASER – no dye/LASER application (94 J/cm2), CCLED – dye/LED application (94 J/cm2) and CCLASER – dye/LASER application (94 J/cm2). The dye used was methylene blue at 10 mM. Dentin scrapes were harvested from each fragment and prepared for counts of colony forming units (CFU)/mL. The data were analyzed using Kruskal–Wallis, followed by Student–Newman–Keuls (⍺ =0.05). Results: Regarding SM, groups CCLASER and CCLED showed a significant reduction in CFU/mL, which was statistically superior to the SCLASER, SCLED and C groups. Regarding LC, the groups CCLASER and CCLED caused a significant reduction in CFU/mL when compared with SCLASER, which showed intermediate values. SCLED and C had a lesser effect on reducing CFU/mL, where the former showed values similar to those of SCLASER. Conclusions: In conclusion, PDT combined with LASER or LED and methylene blue had a significant antimicrobial effect on cariogenic bacteria in the dentin.

scholarly journals Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

2015 ◽  
Vol 08 (04) ◽  
pp. 1550017 ◽  
Author(s):  
Xuewei Jiang ◽  
Zhichao Fan ◽  
Yili Yu ◽  
Chenying Shao ◽  
Yuanzhen Suo ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Toluidine Blue ◽  
Pathogenic Bacteria ◽  
Light Emitting Diode ◽  
Red Light ◽  
In Vitro Screening ◽  
Light Emitting ◽  
Led Phototherapy ◽  
Prevotella Melaninogenica

Photodynamic therapy (PDT) has been commonly used in treating many diseases, such as cancer and infectious diseases. We investigated the different effects of PDT on three main pathogenic bacteria of periodontitis — Prevotella melaninogenica (P.m.), Porphyromonas gingivalis (P.g.) and Aggregatibacter actinomycetemcomitans (A.a.). The portable red light-emitting diode (LED) phototherapy device was used to assess the exogenous PDT effects with different light doses and photosensitizer concentrations (Toluidine blue O, TBO). The portable blue LED phototherapy device was used to assess the endogenous PDT effects with the use of endogenous photosensitizers (porphyrin) under different light doses. We found out that both exogenous and endogenous PDT were able to restrict the growth of all the three bacteria significantly. Moreover, the optimal PDT conditions for these bacteria were obtained through this in vitro screening and could guide the clinical PDT on periodontitis.

scholarly journals Photophysicochemical Properties and In Vitro Phototherapeutic Effects of Iodoquinoline- and Benzothiazole-Derived Unsymmetrical Squaraine Cyanine Dyes

2019 ◽  
Vol 9 (24) ◽  
pp. 5414 ◽  
Author(s):  
Sofia Friães ◽  
Eurico Lima ◽  
Renato E. Boto ◽  
Diana Ferreira ◽  
José R. Fernandes ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Cell Lines ◽  
Light Emitting Diode ◽  
Cyanine Dyes ◽  
Quantum Yields ◽  
Light Emitting ◽  
Diode System ◽  
Hepg2 Cell Lines

The search to replace conventional cancer treatment therapies, such as chemotherapy, radiotherapy and surgery has led over the last ten years, to a substantial effort in the development of several classes of photodynamic therapy photosensitizers with desired photophysicochemical and photobiological properties. Herein we report the synthesis of 6-iodoquinoline- and benzothiazole-based unsymmetrical squaraine cyanine dyes functionalized with amine groups located in the four-membered central ring. Their photodegradation and singlet oxygen production ability, as well as their in vitro photocytotoxicity against Caco-2 and HepG2 cell lines using a 630.8 ± 0.8 nm centered light-emitting diode system, were also investigated. All photosensitizer candidates displayed strong absorption within the tissue transparency spectral region (650–850 nm). The synthesized dyes were found to have moderate light stability. The potential of these compounds is evidenced by their cytotoxic activity against both tumor cell lines, highlighting the zwitterionic unsubstituted dye, which showed more intense photodynamic activity. Although the singlet oxygen quantum yields of these iodinated derivatives are considered low, it could be concluded that their introduction into the quinoline heterocycle was highly advantageous as it played a role in increasing selective cytotoxicity in the presence of light. Thus, the novel synthesized dyes present photophysicochemical and in vitro photobiological properties that make them excellent photosensitizer candidates for photodynamic therapy.

scholarly journals Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

2020 ◽  
Vol 47 (1) ◽  
pp. 70-77
Author(s):  
Choa Park ◽  
Howon Park ◽  
Juhyun Lee ◽  
Hyunwoo Seo ◽  
Siyoung Lee
Keyword(s):  
Photodynamic Therapy ◽  
Laser Scanning ◽  
Light Emitting Diode ◽  
Antimicrobial Effect ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Control Group ◽  
Antimicrobial Photodynamic Therapy ◽  
Group Dynamic ◽  
Light Emitting

The present study is aimed to assess the effect of antimicrobial photodynamic therapy (aPDT) on <i>Streptococcus mutans</i> biofilm through teeth whitening light emitting diode (LED).<br/>Planktonic and dynamic biofilm state cultures of <i>S. mutans</i> were used. Erythrosine 20 μM/L was used as the photosensitizer. Irradiation was performed by exposing cultures to clinic and homecare whitening LEDs for 15 minutes. The viability was measured through Colony Forming Unit counts and confocal laser scanning microscopy.<br/>aPDT using whitening LEDs and erythrosine significantly decreased the CFU count of <i>S. mutans</i> compared to that in the control group. Dynamic biofilm group showed more resistant features to aPDT compared with planktonic state. Clinic and homecare whitening LED device showed similar antimicrobial effect.<br/>The whitening LED, which could irradiate the entire oral arch, showed a significant photodynamic effect on cariogenic <i>S. mutans</i> biofilm. aPDT mediated by erythrosine and LEDs used for teeth whitening exhibited promising antimicrobial activity.

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