Oxidative damage in forage rape ( Brassica napus L.) seeds following heat stress during seed development

2019 ◽  
Vol 206 (1) ◽  
pp. 101-117
Author(s):  
Muhammad Rashid ◽  
John G. Hampton ◽  
Martin L. Shaw ◽  
M. Philip Rolston ◽  
Khan Mir Khan ◽  
...  
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Oxidative Damage ◽  
Seed Development ◽  
Brassica Napus L ◽  
Forage Rape

Forage rape (Brassica napus L) seed quality: Impact of heat stress in the field during seed development

2018 ◽  
Vol 217 ◽  
pp. 172-179 ◽  
Author(s):  
Muhammad Rashid ◽  
John G. Hampton ◽  
M. Phil Rolston ◽  
Jason A.K. Trethewey ◽  
David J. Saville
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Seed Development ◽  
Seed Quality ◽  
Brassica Napus L ◽  
Forage Rape

Heat stress during seed development affects forage brassica (Brassica napus L.) seed quality

2017 ◽  
Vol 204 (2) ◽  
pp. 147-154 ◽  
Author(s):  
M. Rashid ◽  
J. G. Hampton ◽  
M. P. Rolston ◽  
K. M. Khan ◽  
D. J. Saville
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Seed Development ◽  
Seed Quality ◽  
Brassica Napus L

scholarly journals Transient daily heat stress during the early reproductive phase disrupts pod and seed development in Brassica napus L.

2020 ◽  
Author(s):  
Sheng Chen ◽  
Katia Stefanova ◽  
Kadambot H. M. Siddique ◽  
Wallace A. Cowling
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Seed Development ◽  
Brassica Napus L ◽  
Reproductive Phase

Characterising canola pollen germination across a temperature gradient

2016 ◽  
Vol 67 (4) ◽  
pp. 317 ◽  
Author(s):  
Malcolm J. Morrison ◽  
Allison Gutknecht ◽  
John Chan ◽  
S. Shea Miller
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Thermal Gradient ◽  
Pollen Germination ◽  
Limiting Factor ◽  
Major Goal ◽  
Brassica Napus L ◽  
Influence Of Temperature ◽  
Heat Stress Tolerance ◽  
Stress Susceptibility

If predictions are correct, heat stress during reproduction will become a yield limiting factor in many world crops and breeding heat stress tolerance a major goal. The objective of our paper was to highlight a novel system to investigate the influence of temperature (T) on pollen germination using a thermal gradient PCR programmed to establish differential Ts across 12 wells of a PCR plate. Seven cultivars of Brassica napus L. were grown through flowering in a cool growth cabinet (20/15°C day/night) or a heat stress cabinet (HST, 27/22°C day/night). Pollen from each cultivar × cabinet combination was aspirated from 6 opened flowers, and suspended in germination media. Drops of the pollen suspension were floated on media in each well, and the PCR T was set to 30°C with a gradient of ± 10°C, creating a range from ~20 to 40°C from left to right. After an 8 h treatment, the pollen germination (pg, %) and pollen tube growth score (ptg, 1–5) were evaluated using a microscope. There were significant differences among cultivars for pg and ptg score and significant differences among well T for pg and ptg score. Pollen tubes grew best at T from 20 to 23°C. Well T exceeding 33°C reduced pg and ptg score, although 3 of the 8 cultivars had good pg even at 36°C. HST >29°C, in a growth cabinet, generally resulted in B. napus raceme sterility, although our experiment showed that pollen was still capable of germinating up to 33°C, indicating that pollen germination may not be the only reason for heat stress susceptibility.

scholarly journals Comparative Transcriptomics Analysis of Brassica napus L. during Seed Maturation Reveals Dynamic Changes in Gene Expression between Embryos and Seed Coats and Distinct Expression Profiles of Acyl-CoA-Binding Proteins for Lipid Accumulation

2019 ◽  
Vol 60 (12) ◽  
pp. 2812-2825 ◽  
Author(s):  
Pan Liao ◽  
Helen K Woodfield ◽  
John L Harwood ◽  
Mee-Len Chye ◽  
Simon Scofield
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Seed Development ◽  
Binding Proteins ◽  
Expression Profiles ◽  
Lipid Biosynthesis ◽  
Dynamic Changes ◽  
Brassica Napus L ◽  
Oil Accumulation ◽  
Seed Coats

Abstract Production of vegetable oils is a vital agricultural resource and oilseed rape (Brassica napus) is the third most important oil crop globally. Although the regulation of lipid biosynthesis in oilseeds is still not fully defined, the acyl-CoA-binding proteins (ACBPs) have been reported to be involved in such metabolism, including oil accumulation, in several plant species. In this study, progressive changes in gene expression in embryos and seed coats at different stages of seed development were comprehensively investigated by transcriptomic analyses in B. napus, revealing dynamic changes in the expression of genes involved in lipid biosynthesis. We show that genes encoding BnACBP proteins show distinct changes in expression at different developmental stages of seed development and show markedly different expression between embryos and seed coats. Both isoforms of the ankyrin-repeat BnACBP2 increased during the oil accumulation period of embryo development. By contrast, the expression of the three most abundant isoforms of the small molecular mass BnACBP6 in embryos showed progressive reduction, despite having the highest overall expression level. In seed coats, BnACBP3, BnACBP4 and BnACBP5 expression remained constant during development, whereas the two major isoforms of BnACBP6 increased, contrasting with the data from embryos. We conclude that genes related to fatty acid and triacylglycerol biosynthesis showing dynamic expression changes may regulate the lipid distribution in embryos and seed coats of B. napus and that BnACBP2 and BnACBP6 are potentially important for oil accumulation.

scholarly journals Heat stress during seed filling interferes with sulfur restriction on grain composition and seed germination in oilseed rape (Brassica napus L.)

2015 ◽  
Vol 6 ◽  
Author(s):  
Sophie Brunel-Muguet ◽  
Philippe D'Hooghe ◽  
Marie-Paule Bataillé ◽  
Colette Larré ◽  
Tae-Hwan Kim ◽  
...  
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Seed Germination ◽  
Oilseed Rape ◽  
Grain Composition ◽  
Brassica Napus L ◽  
Seed Filling

scholarly journals Role of salicylic acid in heat stress tolerance in tri-genomic Brassica napus L.

2020 ◽  
Vol 33 (1) ◽  
pp. 13-20
Author(s):  
Muhammad Awais Ghani ◽  
Muhammad Mehran Abbas ◽  
Basharat Ali ◽  
Khurram Ziaf ◽  
Muhammad Azam ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Heat Stress ◽  
High Temperature ◽  
Brassica Napus ◽  
Biochemical Analysis ◽  
Foliar Application ◽  
Brassica Napus L ◽  
Elevated Activity ◽  
Heat Stress Tolerance

Tri-genomic Brassica napus L.wasdeveloped by the cross between Brassica napusand Brassica nigra. The crop is animportant source of vegetable seed oil in Pakistan,after cotton. The low oilseed rape yield is attributed to high temperature in the production zones. Interspecific hybridization using these two speciescan be helpful to produce heat resistant hybrids. On the other hand, it has been found that foliar application of different plant growth regulators can be used to reduce the heat stress in Brassica. The objectiveof this studywas to test the response of three different tri-genomic hybrids to high temperature stressat seedling stage. Seedlings were foliar sprayed with 0.13 mM salicylic acid (SA) prior to exposure tohigh temperatureat two true leaf stage. The plants were harvested after 30 days of sowing for growth and biochemical analysis. Plants ofV38 showed the highest values for all morphological traits and biochemical activities among the threehybrids. In general, plants exposed to the temperature stress exhibited a significant decline in growth, chlorophyll content and enzyme activity.Foliar application of SA significantly improved leaf and root biomass under heat stress.Further, antioxidativeenzyme activities significantly increased in response to SA either compared to control or to plants exposed to temperature stress.It is concluded thatapplication of salicylic acid elevated activity of antioxidative enzymes and was helpful in mitigating the detrimental effects of high temperature inoil seed rape.

EFFECT OF FROST ON HULL STRUCTURE OF CANOLA SEEDS

1984 ◽  
Vol 64 (4) ◽  
pp. 841-848 ◽  
Author(s):  
J. T. MILLS ◽  
L. VAN CAESEELE ◽  
J. K. DAUN
Keyword(s):  
Brassica Napus ◽  
Seed Development ◽  
Frost Damage ◽  
Canola Seed ◽  
Normal Appearance ◽  
Arrested Development ◽  
Brassica Napus L ◽  
Secondary Thickening ◽  
Hull Structure ◽  
Damage Type

Two lots of severely frost-damaged canola seeds (Brassica napus L. ’Regent’) were categorized according to damage type. Seeds from these lots ranged in gross morphology from normal appearance to severely shrivelled with reduced diameter and white patches over the surface. The white color of these patches was caused by accumulation of starch-like granules in the epidermal layers. Frost-damaged hulls showed a variety of symptoms resulting from arrested development. In addition to an accumulation of starch-like granules in the epidermis, frost-damaged hulls displayed abnormal secondary thickening in the palisade.Key words: Frost-damage, canola, seed development, hull structure

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