scholarly journals Role of salicylic acid in heat stress tolerance in tri-genomic Brassica napus L.

2020 ◽  
Vol 33 (1) ◽  
pp. 13-20
Author(s):  
Muhammad Awais Ghani ◽  
Muhammad Mehran Abbas ◽  
Basharat Ali ◽  
Khurram Ziaf ◽  
Muhammad Azam ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Heat Stress ◽  
High Temperature ◽  
Brassica Napus ◽  
Biochemical Analysis ◽  
Foliar Application ◽  
Brassica Napus L ◽  
Elevated Activity ◽  
Heat Stress Tolerance

Tri-genomic Brassica napus L.wasdeveloped by the cross between Brassica napusand Brassica nigra. The crop is animportant source of vegetable seed oil in Pakistan,after cotton. The low oilseed rape yield is attributed to high temperature in the production zones. Interspecific hybridization using these two speciescan be helpful to produce heat resistant hybrids. On the other hand, it has been found that foliar application of different plant growth regulators can be used to reduce the heat stress in Brassica. The objectiveof this studywas to test the response of three different tri-genomic hybrids to high temperature stressat seedling stage. Seedlings were foliar sprayed with 0.13 mM salicylic acid (SA) prior to exposure tohigh temperatureat two true leaf stage. The plants were harvested after 30 days of sowing for growth and biochemical analysis. Plants ofV38 showed the highest values for all morphological traits and biochemical activities among the threehybrids. In general, plants exposed to the temperature stress exhibited a significant decline in growth, chlorophyll content and enzyme activity.Foliar application of SA significantly improved leaf and root biomass under heat stress.Further, antioxidativeenzyme activities significantly increased in response to SA either compared to control or to plants exposed to temperature stress.It is concluded thatapplication of salicylic acid elevated activity of antioxidative enzymes and was helpful in mitigating the detrimental effects of high temperature inoil seed rape.

Characterising canola pollen germination across a temperature gradient

2016 ◽  
Vol 67 (4) ◽  
pp. 317 ◽  
Author(s):  
Malcolm J. Morrison ◽  
Allison Gutknecht ◽  
John Chan ◽  
S. Shea Miller
Keyword(s):  
Heat Stress ◽  
Brassica Napus ◽  
Thermal Gradient ◽  
Pollen Germination ◽  
Limiting Factor ◽  
Major Goal ◽  
Brassica Napus L ◽  
Influence Of Temperature ◽  
Heat Stress Tolerance ◽  
Stress Susceptibility

If predictions are correct, heat stress during reproduction will become a yield limiting factor in many world crops and breeding heat stress tolerance a major goal. The objective of our paper was to highlight a novel system to investigate the influence of temperature (T) on pollen germination using a thermal gradient PCR programmed to establish differential Ts across 12 wells of a PCR plate. Seven cultivars of Brassica napus L. were grown through flowering in a cool growth cabinet (20/15°C day/night) or a heat stress cabinet (HST, 27/22°C day/night). Pollen from each cultivar × cabinet combination was aspirated from 6 opened flowers, and suspended in germination media. Drops of the pollen suspension were floated on media in each well, and the PCR T was set to 30°C with a gradient of ± 10°C, creating a range from ~20 to 40°C from left to right. After an 8 h treatment, the pollen germination (pg, %) and pollen tube growth score (ptg, 1–5) were evaluated using a microscope. There were significant differences among cultivars for pg and ptg score and significant differences among well T for pg and ptg score. Pollen tubes grew best at T from 20 to 23°C. Well T exceeding 33°C reduced pg and ptg score, although 3 of the 8 cultivars had good pg even at 36°C. HST >29°C, in a growth cabinet, generally resulted in B. napus raceme sterility, although our experiment showed that pollen was still capable of germinating up to 33°C, indicating that pollen germination may not be the only reason for heat stress susceptibility.

scholarly journals Impact of Foliar Application with Salicylic Acid on Biochemical Characters of Canola Plants under Cold Stress Condition

2016 ◽  
Vol 8 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Hamed KESHAVARZ ◽  
Seyed Ali Mohammad MODARRES SANAVY ◽  
Ramin SADEGH GHOL MOGHADAM
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Acid Treatment ◽  
Stress Condition ◽  
Foliar Application ◽  
Total Chlorophyll ◽  
Salicylic Acid Treatment ◽  
Biochemical Traits ◽  
Brassica Napus L ◽  
Salicylic Acid Concentration

In this study the effect of foliar application of salicylic acid on the chlorophyll content, antioxidant enzymes activity, and the content of solute protein and proline were investigated in two canola varieties (Brassica napus L., cv ‘RGS’ and ‘Licord’) leaves during 0, 24, and 48 hours after salicylic acid treatment. The results showed that the content of total chlorophyll was decreased in ‘RGS’ cultivar during the experiment and this process was related with increasing of salicylic acid concentration. The activity of superoxide dismutase, peroxidase, and also lipid peroxidation were increased significantly after 48 hours compared with the first day. The results of catalase activity showed that, this trait was decreased 24 hours after salicylic acid treatment and this decrease was related with salicylic concentration. The content of protein in both cultivars slightly changed and plants treated with salicylic acid had more protein content, by contrast proline was greatly affected by salicylic acid treatment and its content was the highest 24 hours after treatment. According to the present findings the application of salicylic acid has useful effects on the biochemical traits of Brassica napus cultivars. Therefore it may be effective for the improvement of plant growth in cold regions.

scholarly journals Salicylic Acid Phytohormone Decreased the Toxicity Damage of Exogenous Lead Absorption in Seedlings of an Oilseed Plant, Brassica napus L. Grown in Hydroponic Conditions

2019 ◽  
pp. 1-15
Author(s):  
Mahdi Khozaei ◽  
Shiva Boroumand Jazi
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Soluble Sugars ◽  
Lead Toxicity ◽  
Chlorophyll Synthesis ◽  
Dry Mass ◽  
Inhibitory Effects ◽  
Brassica Napus L ◽  
Carbohydrate Levels

Oilseed plant, Brassica napus L. seedlings grown in hydroponic condition with different concentrations of Pb were treated with salicylic acid (SA) to investigate the role of exogenous salicylic acid in alleviating lead toxicity on biochemical and physiological activities of the plant. The results showed that application of different concentrations of Pb increased soluble sugars and reduced carbohydrate levels significantly in roots and shoots of the plants. The stress induced by application of Pb triggered significant inhibitory effects on growth and chlorophyll synthesis induced on the production of protein and proline and enhanced the levels of antioxidant activity. Salicylic acid (SA) treated plants showed alleviation increasing total dry mass, leaf area, shoot and root length as well as leaf total chlorophyll content in responses to Pb stress. Results revealed the importance of salicylic acid (SA) activity in enabling plants to reduce the soluble sugars and increase of insoluble sugar in heavy -metal-stressed plants. The content of proline and proteins were also reduced in plants were treated with salicylic acid. Our data provide evidence that salicylic acid treatment decreased the activity of antioxidant enzymes in plants were exposed to different levels of Pb.

scholarly journals Contrastive response of Brassica napus L. to exogenous salicylic acid, selenium and silicon supplementation under water stress

2015 ◽  
Vol 67 (2) ◽  
pp. 397-404 ◽  
Author(s):  
Ghader Habibi
Keyword(s):  
Lipid Peroxidation ◽  
Salicylic Acid ◽  
Drought Stress ◽  
Brassica Napus ◽  
Foliar Application ◽  
Dry Mass ◽  
Total Chlorophyll ◽  
Brassica Napus L ◽  
Positive Effect ◽  
Exogenous Salicylic Acid

The present research was designed to determine the effects of exogenous salicylic acid (SA), selenium (Se) and silicon (Si) on the resistance of canola (Brassica napus L. cv Okapi) seedlings to salt stress. Foliar application of SA (0.1 mM) in canola plants under drought stress for 25 days exhibited a significantly positive effect on shoot dry mass and raised the levels of total chlorophyll as well as boosting the activity of superoxide dismutase (SOD) and catalase (CAT). In addition, soil application of silicon (0.35 g Na2SiO3/kg soil) had ameliorative effects on canola root growth under drought. It is concluded that SA and Si enhanced the salt tolerance of canola by protecting the cell membrane against lipid peroxidation. However, the foliar application of Se (10 mg/l) had no ameliorative effects on canola growth and antioxidant capacity under drought stress, as could be judged by accumulation of malondialdehyde (MDA).

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