Isolation and characterization of chitin-degrading micro-organisms from the faeces of Goeldi's monkey, Callimico goeldii

Active probiotic organisms are good bacteria considered to be live micro-organisms that are obtained from fermented foods. Evidence has shown that probiotics are essential in human health. This studys primary objective is to isolate and characterize the active probiotic organisms present in certain fermented food samples. Maize, African oil bean, and castor oil were subjected to analysis. The result found the presence of active probiotic organisms such as Pedioccocus, Lactobacillus, Micrococcus, and Bacillus species. The study concluded that these organisms are responsible for the fermentation of carbohydrates and protein-rich seeds.

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Isolation and Characterization of Micro-organisms with Industrial Importance From Sisal Bole Rots

Tanzania Journal of Engineering and Technology ◽

10.52339/tjet.v34i1.456 ◽

2013 ◽

Vol 34 (1) ◽

pp. 26-35

Author(s):

Consolatha J.N. Mhaiki ◽

Enock Masanja ◽

Jamidu H.Y. Katima ◽

Gunaraths Rajarao ◽

Gunnel Dalhammar

Keyword(s):

Polymerase Chain Reaction ◽

Chain Reaction ◽

Isolation And Characterization ◽

Brevundimonas Diminuta ◽

Industrial Use ◽

Brevundimonas Vesicularis ◽

Polymerase Chain ◽

The Individual ◽

Micro Organisms

Investigation of microorganisms naturally acclimatized to Agave hybrid H 11648 (sisal bole rot) was conducted, with the aim of isolating and characterizing Aspergillus niger strains for industrial use. Microorganism were identified morphologically and then confirmation made by polymerase chain reaction (PCR). Results showed the existence of four major groups, listed in order of abundances as follows; Aspergilli (36.0±0.8) %, Penicillin (28.0±0.1) %, Yeast (15.0±1.6) %and Fusarium (10.0±0.12) %. The main groups of Aspergilli strains were A. nidulans, A. tamari and A. niger in ratios (3:2:2), respectively. Several endo-spore forming non-enteric gram (-) rods and coccid bacteria identified by API20 NE identification systemincluded,Brevundimonas diminuta sp, Shewanella putrefaciens sp, Brevundimonas vesicularis sp and Pasteurella sp. Results showed that sisal bole rot stems hosts a high bio-diversity of microorganism species other than A. niger. Exploitation of the individual strains is recommended. This could eventually produce strains forprecursors of industrially and therapeutically metabolites.

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Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp. nov. and Bifidobacterium leontopitheci sp. nov.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.004032 ◽

2020 ◽

Vol 70 (4) ◽

pp. 2288-2297 ◽

Cited By ~ 13

Author(s):

Sabrina Duranti ◽

Gabriele Andrea Lugli ◽

Alice Viappiani ◽

Leonardo Mancabelli ◽

Giulia Alessandri ◽

...

Keyword(s):

Type Species ◽

Phylogenetic Analyses ◽

Bifidobacterium Bifidum ◽

Content Type ◽

Link Type ◽

Callimico Goeldii ◽

Goeldi's Monkey ◽

Type Strains ◽

Rrna Sequences

Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii). Isolates were shown to be Gram-positive, non-motile, non-sporulating, facultative anaerobic and d-fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively. Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species. Thus, Bifidobacterium cebidarum sp. nov. (2176BT=LMG 31469T=CCUG 73785T) and Bifidobacterium leontopitheci sp. nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.

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Isolation and characterization of the gene encoding an aminopeptidase involved in the selective toxicity of ascamycin toward Xanthomonas campestris pv. citri

Biochemical Journal ◽

10.1042/bj3190099 ◽

1996 ◽

Vol 319 (1) ◽

pp. 99-102 ◽

Cited By ~ 6

Author(s):

Tatsuhiko SUDO ◽

Kiwako SHINOHARA ◽

Naoshi DOHMAE ◽

Koji TAKIO ◽

Ron USAMI ◽

...

Keyword(s):

Xanthomonas Campestris ◽

Selective Toxicity ◽

Gene Encoding ◽

Calculated Molecular Mass ◽

Isolation And Characterization ◽

Acid Protein ◽

Micro Organisms ◽

Proline Iminopeptidase

An aminopeptidase gene named XAP has been isolated from Xanthomonas campestris pv. citri, a plant pathogenic bacterium. The bacterium is one of the rare micro-organisms susceptible to ascamycin, an aminoacyl nucleoside antibiotic that inhibits protein synthesis. Sequence analysis reveals that the gene encodes a 311 amino acid protein with a calculated molecular mass of 35134 Da and approx. 50% identity for amino acids to the proline iminopeptidase from Neisseria gonorrhoeae. The XAP gene product, Xap, expressed in Escherichia coli has proline iminopeptidase activity as well as ascamycin dealanylating activity in vitro.

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Living hell: Life at high temperatures

The Biochemist ◽

10.1042/bio02701006 ◽

2005 ◽

Vol 27 (1) ◽

pp. 6-10 ◽

Cited By ~ 2

Author(s):

Kazem Kashefi

Keyword(s):

High Temperatures ◽

Hydrothermal Vents ◽

Scientific Community ◽

Early Earth ◽

Isolation And Characterization ◽

Hot Environments ◽

Micro Organisms

Modern hot environments, such as those found in hydrothermal vents, are of great interest to the scientific community because they resemble those ancient environments where life first arose on Earth and also share many characteristics with environments in other planets where life may have actually existed. Evidence to date strongly indicates that Fe(III) respiration may have been one of the first, if not the first, forms of respiration in a hot, early Earth. The abundance of Fe(III) minerals in many modern and ancient hot environments suggests that studies of Fe(III)-reducing hyperthermophilic micro-organisms are likely to be instrumental for our understanding of how life originated and evolved at high temperatures. The isolation and characterization of novel hyperthermophilic, Fe(III)-reducing micro-organisms has greatly increased our understanding of how microbes can live and thrive in such inhospitable environments. The study of these amazing microbes provides clues as to how life might have arisen on Earth and has implications for our search for traces of life in other planets.

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