Proposal of a novel selective enrichment broth, NCT‐mTSB, for isolation of Escherichia albertii from poultry samples

2021 ◽  
Author(s):  
Yuki Wakabayashi ◽  
Kazuko Seto ◽  
Masashi Kanki ◽  
Tetsuya Harada ◽  
Kentaro Kawatsu
Keyword(s):  
Enrichment Broth ◽  
Selective Enrichment ◽  
Selective Enrichment Broth

Improvement of a new selective enrichment broth for culturing Salmonella in ready‐to‐eat fruits and vegetables

2020 ◽  
Vol 40 (5) ◽  
Author(s):  
Jiajia Wan ◽  
Zhaoxin Lu ◽  
Xiaomei Bie ◽  
Fengxia Lv ◽  
Haizhen Zhao
Keyword(s):  
Fruits And Vegetables ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Selective Enrichment Broth

scholarly journals Detection of Burkholderia pseudomallei in Sputum using Selective Enrichment Broth and Ashdown’s Medium at Kampong Cham Provincial Hospital, Cambodia

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 302 ◽  
Author(s):  
Somary Nhem ◽  
Joanne Letchford ◽  
Chea Meas ◽  
Sovanndeth Thann ◽  
James C. McLaughlin ◽  
...  
Keyword(s):  
Burkholderia Pseudomallei ◽  
Low Frequency ◽  
Culture Methods ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Provincial Hospital ◽  
Testing Protocol ◽  
Standard Culture ◽  
Diagnostic Microbiology ◽  
Selective Enrichment Broth

Melioidosis infection, caused by Burkholderia pseudomallei, is increasingly reported in Cambodia. We hypothesized that implementation of an enhanced sputum testing protocol in a provincial hospital diagnostic microbiology laboratory would increase detection of B. pseudomallei. We tested 241 sputum specimens that were deemed acceptable for culture, comparing culture in selective enrichment broth followed by sub-culture on Ashdown’s medium to standard culture methods. Two specimens (0.8%) were positive for B. pseudomallei using the enhanced protocol whereas one specimen (0.4%) was positive using standard methods. These findings demonstrate that B. pseudomallei is rarely detected in sputum at this hospital. The low frequency of B. pseudomallei in sputum specimens precludes drawing any conclusions about the relative benefits of an enhanced sputum testing protocol at this site. Promoting clinician awareness of the infection and encouraging utilization of diagnostic microbiology services are likely to be important factors in facilitating identification of melioidosis.

Growth of Yersinia enterocolitica and Y. pseudotuberculosis in Yersinia selective enrichment broth according to Ossmer

2012 ◽  
Vol 89 (3) ◽  
pp. 198-200 ◽  
Author(s):  
Aruni Premaratne ◽  
Teresa Wilson ◽  
Nicola King ◽  
J. Andrew Hudson
Keyword(s):  
Yersinia Enterocolitica ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Selective Enrichment Broth

scholarly journals Comparison of the Rodac Imprint Method to Selective Enrichment Broth for Recovery of Vancomycin-Resistant Enterococci and Drug-Resistant Enterobacteriaceae from Environmental Surfaces

2000 ◽  
Vol 38 (12) ◽  
pp. 4646-4648 ◽  
Author(s):  
Donna M. Hacek ◽  
William E. Trick ◽  
Susan M. Collins ◽  
Gary A. Noskin ◽  
Lance R. Peterson
Keyword(s):  
Amphotericin B ◽  
Brain Heart Infusion ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Vancomycin Resistant Enterococci ◽  
Environmental Surfaces ◽  
Vancomycin Resistant ◽  
Selective Enrichment Broth

We compared the Rodac imprint technique to selective enrichment broth for detecting vancomycin-resistant enterococci (VRE) and multidrug-resistant Enterobacteriaceae (MDRE) on surfaces. Rodac plates contained tryptic soy agar with 5% sheep blood, vancomycin (6 μg/ml), ceftazidime (2 μg/ml), amphotericin B (2 μg/ml), and clindamycin (1 μg/ml). Two types of broth were used: brain heart infusion (BHI) and BHI plus vancomycin (6 μg/ml) and ceftazidime (2 μg/ml) (BHIVC). Of the 46 surfaces cultured for VRE, 12 (26%) were positive. Of the 12 VRE-positive surfaces, 11 (92%) grew from Rodac, 8 (67%) grew from BHIVC, and 7 (58%) grew from BHI. A larger study is needed for MDRE, as only 4 of 43 surfaces were MDRE positive. The Rodac imprint technique successfully recovered VRE from environmental surfaces.

Selective enrichment broth medium for isolation of Campylobacter jejuni.

1983 ◽  
Vol 17 (5) ◽  
pp. 853-855 ◽  
Author(s):  
W T Martin ◽  
C M Patton ◽  
G K Morris ◽  
M E Potter ◽  
N D Puhr
Keyword(s):  
Campylobacter Jejuni ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Selective Enrichment Broth

A New Method for Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii Detection using a Novel Chromogenic Agar

2020 ◽  
Author(s):  
Paul Nguyen ◽  
Oscar Juárez ◽  
Lawrence Restaino
Keyword(s):  
Detection System ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Food Samples ◽  
Test Method ◽  
Good Growth ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Arcobacter Butzleri ◽  
Selective Enrichment Broth

Arcobacter species are Gram-negative rods that have been implicated in food- and waterborne illness. Although various cultural isolation methods have been proposed, the current procedures are unable to fully suppress growth of background microbiota present in food samples which inhibits Arcobacter isolation. The purpose of this study was to develop a selective enrichment broth and chromogenic plating medium to detect three Arcobacter species that have been recognized as emerging foodborne pathogens: Arcobacter butzleri , Arcobacter cryaerophilus and Arcobacter skirrowii . The developed Nguyen-Restaino-Juárez Arcobacter detection system consists of a selective enrichment broth (NRJ-B) and a selective/differential plating media (NRJ-M). The protocol of the detection method was determined by evaluating growth of A. butzleri , A. cryaerophilus and A. skirrowii under various temperature (30, 35 and 42ᴼC) and incubation (aerobic, microaerophilic and anaerobic) conditions. Additionally, 47 Arcobacter strains and 39 non- Arcobacter strains were tested in the inclusivity and exclusivity evaluations of NRJ-B and NRJ-M. Overall, the study determined the optimal growth conditions of Arcobacter species using the NRJ- Arcobacter detection system was aerobic incubation at 30ᴼC. NRJ-B supported good growth of A. butzleri , A. cryaerophilus , and A. skirrowii while effectively suppressing growth of non- Arcobacter strains after 48 h. Furthermore, NRJ-M yielded 97.8% inclusivity and 100.0% exclusivity using the tested strains and resulted in salmon-pigmented Arcobacter colonies (1.0 to 1.5 mm in diameter) after 72 h. The novel protocol is the first to develop a chromogenic plating media for the isolation of Arcobacter species. This simple and reliable test method would greatly contribute to understanding the distribution of pathogenic Arcobacter species in food samples.

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