VHSV IVb infection and autophagy modulation in the rainbow trout gill epithelial cell line RTgill‐W1

2020 ◽  
Vol 43 (10) ◽  
pp. 1237-1247 ◽  
Author(s):  
Juan‐Ting Liu ◽  
Phuc H. Pham ◽  
Sarah K. Wootton ◽  
Niels C. Bols ◽  
John S. Lumsden

Parasitology ◽  
2014 ◽  
Vol 142 (2) ◽  
pp. 326-331 ◽  
Author(s):  
S. H. McCONNACHIE ◽  
J. SHEPPARD ◽  
G. M. WRIGHT ◽  
D. J. SPEARE

SUMMARYGrowth and propagation of fish-infecting microsporidians within cell culture has been more difficult to achieve than for insect- and human-infecting microsporidians. Fish microsporidia tend to elicit xenoma development rather than diffuse growth in vivo, and this process likely increases host specificity. We present evidence that the fish microsporidian, Loma salmonae, has the capacity to develop xenomas within a rainbow trout gill epithelial cell line (RTG-1). Spore numbers increased over a 4 weeks period within cell culture flasks. Xenoma-like structures were observed using phase contrast microscopy, and then confirmed using transmission electron microscopy. Optimization of the L. salmonae-RTG-1 cell model has important implications in elucidating the process of xenoma development induced by microsporidian parasites.



2011 ◽  
Vol 17 (2) ◽  
pp. e241-e252 ◽  
Author(s):  
A. KAWANO ◽  
C. HAIDUK ◽  
K. SCHIRMER ◽  
R. HANNER ◽  
L.E.J. LEE ◽  
...  


Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.





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