scholarly journals Novel atypical Aeromonas salmonicida bath challenge model for juvenile ballan wrasse ( Labrus bergylta, Ascanius)

2020 ◽  
Author(s):  
Athina Papadopoulou ◽  
Kathryn Garvey ◽  
Tom Hill ◽  
Jose G. Ramirez‐Paredes ◽  
Sean J. Monaghan ◽  
...  
Keyword(s):  
Aeromonas Salmonicida ◽  
Labrus Bergylta ◽  
Bath Challenge

scholarly journals Atypical Aeromonas salmonicida vapA type V and Vibrio spp. are predominant bacteria recovered from ballan wrasse Labrus bergylta in Scotland

2020 ◽  
Vol 140 ◽  
pp. 47-54 ◽  
Author(s):  
A Papadopoulou ◽  
T Wallis ◽  
JG Ramirez-Paredes ◽  
SJ Monaghan ◽  
A Davie ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Skin Lesions ◽  
Aeromonas Salmonicida ◽  
Sequencing Analysis ◽  
Bacterial Isolates ◽  
Positive Individual ◽  
Labrus Bergylta ◽  
Type V ◽  
First Time ◽  
Vibrio Spp

Healthy and/or moribund farmed and wild ballan wrasse Labrus bergylta (>0.5 to 900 g) were sampled from hatcheries (n = 2) and Atlantic salmon Salmo salar cage sites (n = 8) in Scotland between February 2016 and October 2018. Less than half of the sampled individuals (n = 43; 32.3%) had been vaccinated (autogenous polyvalent vaccine; dip and/or injection) against atypical furunculosis (type V and VI), while 20 (15.0%) fish were not vaccinated, and the rest (70 individuals, 52.7%) were of unknown vaccination status. Swab samples from skin lesions, gill, liver, spleen and kidney were inoculated onto a variety of bacteriological agar plates, and bacteriology identification and sequencing analysis was performed on significant bacterial colonies. Atypical Aeromonas salmonicida (aAs) vapA type V was the predominant bacterial species (70/215 bacterial isolates, 32.5% of bacterial samples; 43/117 positive individual fish, 36.8%) isolated in this survey followed by Vibrio species, which were the most geographically prevalent bacteria. Photobacterium indicum/profundum was also isolated from L. bergylta for the first time during this study. The collection of these bacterial isolates provides useful information for disease management. Identifying the aAs isolates involved in disease in ballan wrasse could provide vital information for improving/updating existing autogenous vaccines.

scholarly journals A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, Ascanius) against Aeromonas salmonicida vapA type V

2020 ◽  
Author(s):  
J. Gustavo Ramirez-Paredes ◽  
D. Verner-Jeffreys ◽  
A. Papadopoulou ◽  
S. J Monaghan ◽  
L. Smith ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Clinical Signs ◽  
Aeromonas Salmonicida ◽  
Vibrio Splendidus ◽  
Degree Days ◽  
Experimental Proof ◽  
Labrus Bergylta ◽  
Type V ◽  
Partial Genome ◽  
Autogenous Vaccine

ABSTRACTAtypical Aeromonas salmonicida (aAs) and Vibrionaceae related species are bacteria routinely recovered from diseased ballan wrasse used as cleaner fish in Atlantic salmon farming. Autogenous multivalent vaccines formulated from these microorganisms are widely used by the industry to protect farmed wrasse despite limited experimental proof that they are primary pathogens. In this study, the components of a commercial multivalent injection wrasse vaccine were tested for infectivity, pathogenicity and virulence via intra peritoneal injection at pre-deployment size (25-50g) and the efficacy of the vaccine for protection against aAs assessed. Injection with 3.5×109, 8×109 1.8×109 and 5×109 cfu/fish of Vibrio splendidus, V. ichthyoenteri, Aliivibrio logeii and A. salmonicida, respectively, did not cause significant mortalities, lesions or clinical signs after a period of 14 days. IP injection with both aAs and Photobacterium indicum successfully reproduced the clinical signs and internal lesions observed during natural outbreaks of the disease. Differences in virulence (LD50 at day 8-post infection of 3.6×106 cfu/fish and 1.6×107 cfu/fish) were observed for two aAs vapA type V isolates. In addition, the LD50 for Photobacterium indicum was 2.2×107 cfu/fish. The autogenous vaccine was highly protective against the two aAs vapA type V isolates after 700-degree days of immunisation. The RPSFINAL values for the first isolate were 95 and 91% at 1×106 cfu/fish and 1×107 cfu/fish, respectively, and 79% at 1×107 cfu/fish for the second isolate tested. In addition, significantly higher anti aAs seral antibodies (IgM), were detected by ELISA in vaccinated fish in contrast with control (mock vaccinated) fish. These results suggest wrasse can be effectively immunised and protected against aAs infection by injection with oil adjuvanted vaccines prepared with inactivated homologous isolates. Further work should assess the efficacy of vaccination against other isolates that have proven to be pathogenic such as aAs type VI and Photobacterium indicum and explore the feasibility of immersion vaccination. In addition, a full characterisation of aAs isolates within the same vapA types should be performed as differences in virulence between vapA type V isolates were observed and partial genome analysis indicated small but potentially important genomic differences in these isolates.

scholarly journals A commercial autogenous injection vaccine protects ballan wrasse (Labrus bergylta, Ascanius) against Aeromonas salmonicida vapA type V

2020 ◽  
Vol 107 ◽  
pp. 43-53 ◽  
Author(s):  
J.G. Ramirez-Paredes ◽  
D.W. Verner-Jeffreys ◽  
A. Papadopoulou ◽  
S.J. Monaghan ◽  
L. Smith ◽  
...  
Keyword(s):  
Aeromonas Salmonicida ◽  
Labrus Bergylta ◽  
Type V

Infeksi Aeromonas salmonicida dari Berbagai Wilayah di Indonesia Pada Ikan Mas (Cyprinus carpio)

2011 ◽  
Vol 16 (2) ◽  
Author(s):  
Riza Priyatna ◽  
Soedarmanto Indarjulianto ◽  
Kurniasih Kurniasih
Keyword(s):  
Cyprinus Carpio ◽  
Aeromonas Salmonicida

Aeromonas salmonicida merupakan bakteri penyebab furunculosis pada ikan yang mengakibatkan kerugian ekonomi di dalam budidaya ikan air tawar. Penelitian bertujuan mengetahui gambaran darah ikan Mas (Cyprinus carpio) yang diinfeksi oleh A. salmonicida, juga dilakukan pemeriksaan histopatologi. Sebanyak empat isolat atipikal A. salmonicida telah diisolasi dari ikan di empat daerah di Indonesia yaitu Pontianak, Semarang, Yogyakarta, Jambi dan satu isolat atipikal A. salmonicida subjenis smithia dari ATCC sebagai kontrol. Sebanyak 45 ekor ikan mas berukuran 1215 cm dibagi menjadi lima kelompok. Kelompok 14 diinfeksi dengan A. salmonicida 0,1ml x 10 4 sel/ml secara intraperitoneal dari empat isolat berbeda. Kelompok ikan 5/kontrol tidak dilakukan infeksi bakteri. Isolat A. salmonicida yang berasal dari Pontianak menunjukkan jumlah leukosit total dan kadar hemoglobin meningkat jelas pada hari ke-7 sesudah infeksi, disertai peningkatan jumlah rata-rata heterofil, limfosit dan monosit pada hari ke-7 sesudah infeksi. Berdasarkan hasil pemeriksaan darah menunjukkan bahwa isolat A. salmonicida dari Pontianak merupakan isolat patogen yang menyebabkan reaksi akut jika dibandingkan dengan isolat dari daerah lain. Isolat A. salmonicida dari Yogyakarta menyebabkan perubahan patologi paling ringan. Isolat dari Jambi dan Pontianak menyebabkan lesi kulit hingga lapisan otot, epicarditis mulai hari ke-3 sesudah infeksi.

Changes in Immunological Parameters and Disease Resistance in Juvenile Coho Salmon (Oncorhynchus kisutch) in Response to Dehydroabietic Acid Exposure under Varying Thermal Conditions

2004 ◽  
Vol 39 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Keith B. Tierney ◽  
Eric Stockner ◽  
Christopher J. Kennedy
Keyword(s):  
Coho Salmon ◽  
Thermal Conditions ◽  
Dehydroabietic Acid ◽  
Aeromonas Salmonicida ◽  
Acclimation Temperature ◽  
Dependent Manner ◽  
Immunological Parameters ◽  
Cold Temperatures ◽  
Temperature Shock ◽  
Exposure Temperature

Abstract This study explored the effects of a sublethal 96-h dehydroabietic acid (DHAA) exposure on aspects of the immune system of juvenile coho salmon under varying temperature conditions. Coho were exposed to DHAA concentrations below the determined LC50 value of 0.94 mg/L (95% confidence limits of 0.81 to 1.24 mg/L) for 96 h at either their acclimation temperature (8 or 18°C), or during an acute warm-shock (8 to 18°C) or cold-shock (18 to 8°C). Acclimation temperature alone significantly affected hematocrit (Hct), neutrophil respiratory burst activity (RBA) and leucocyte proportions. With temperature-shock, leucocrit (Lct), RBA and leucocyte proportions were altered. All parameters were affected by DHAA exposure, but not always in a dose-dependent manner. Across groups, DHAA caused Hct, lysozyme, thrombocyte, neutrophil and monocyte proportions to increase, and Lct, RBA and lymphocyte proportions to decrease. DHAA-temperature interactions resulted in the exacerbation of DHAA-induced effects. Exposure temperature had the most significant effect on the susceptibility of coho to Aeromonas salmonicida; fish were more susceptible at cold temperatures and when subjected to a temperature-shock compared to their respective controls. DHAA exposure modulated the response of temperature-shocked fish to this pathogen.

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