Challenge models for atypical Aeromonas salmonicida and Vibrio anguillarum in farmed Ballan wrasse (Labrus bergylta ) and preliminary testing of a trial vaccine against atypical Aeromonas salmonicida

2016 ◽  
Vol 39 (10) ◽  
pp. 1257-1261 ◽  
Author(s):  
E Biering ◽  
Ø Vaagnes ◽  
B Krossøy ◽  
S Gulla ◽  
DJ Colquhoun
Keyword(s):  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Preliminary Testing ◽  
Labrus Bergylta

Ultraviolet Treatment of Water for Destruction of Five Gram-Negative Bacteria Pathogenic to Fishes

1977 ◽  
Vol 34 (8) ◽  
pp. 1244-1249 ◽  
Author(s):  
G. L. Bullock ◽  
H. M. Stuckey
Keyword(s):  
Particulate Matter ◽  
Ultraviolet Irradiation ◽  
Vibrio Anguillarum ◽  
Spring Water ◽  
Aeromonas Salmonicida ◽  
Water Disinfection ◽  
Gram Negative Bacteria ◽  
Fish Pathogens ◽  
Gram Negative ◽  
Ultraviolet Treatment

Filtration (25 nm) and ultraviolet irradiation dosages of 13,100–29,400 microwatt seconds per square centimetre (μW∙s∙cm−2) effected a 99.98–100% reduction of five gram-negative fish pathogens — Aeromonas salmonicida, A. hydrophila, Vibrio anguillarum, Pseudomonas fluorescens, and the enteric redmouth organism in 12.5 °C clear spring water or spring water containing particulate matter. Filtration and a dosage of 4500 μW∙s∙cm−2 killed 99.83–100% of test strains in spring water and 4000–4750 μW∙s∙cm−2 killed 99.33–99.99% in water with particulate matter. Irradiation of unfiltered water containing particulate matter was less effective, especially at dosages of 5000 μW∙s∙cm−2 or less, which killed 97–99.94% of strains. Filtration and 13,100 μW∙s∙cm−2 irradiation of water containing A. salmonicida prevented transmission of furunculosis. Key words: ultraviolet irradiation, bacterial fish pathogens, water disinfection

Application of real-time PCR for early diagnosis of diseases caused by Aeromonas salmonicida, Vibrio anguillarum, and Tenacibaculum maritimum in turbot: A field study

2017 ◽  
Vol 30 (1) ◽  
pp. 76-89 ◽  
Author(s):  
María-José Chapela ◽  
Martiña Ferreira ◽  
Asela Ruiz-Cruz ◽  
Iris Martin-Varela ◽  
Jacobo Fernández-Casal ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Field Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Diagnosis Of Diseases

scholarly journals Atypical Aeromonas salmonicida vapA type V and Vibrio spp. are predominant bacteria recovered from ballan wrasse Labrus bergylta in Scotland

2020 ◽  
Vol 140 ◽  
pp. 47-54 ◽  
Author(s):  
A Papadopoulou ◽  
T Wallis ◽  
JG Ramirez-Paredes ◽  
SJ Monaghan ◽  
A Davie ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Skin Lesions ◽  
Aeromonas Salmonicida ◽  
Sequencing Analysis ◽  
Bacterial Isolates ◽  
Positive Individual ◽  
Labrus Bergylta ◽  
Type V ◽  
First Time ◽  
Vibrio Spp

Healthy and/or moribund farmed and wild ballan wrasse Labrus bergylta (>0.5 to 900 g) were sampled from hatcheries (n = 2) and Atlantic salmon Salmo salar cage sites (n = 8) in Scotland between February 2016 and October 2018. Less than half of the sampled individuals (n = 43; 32.3%) had been vaccinated (autogenous polyvalent vaccine; dip and/or injection) against atypical furunculosis (type V and VI), while 20 (15.0%) fish were not vaccinated, and the rest (70 individuals, 52.7%) were of unknown vaccination status. Swab samples from skin lesions, gill, liver, spleen and kidney were inoculated onto a variety of bacteriological agar plates, and bacteriology identification and sequencing analysis was performed on significant bacterial colonies. Atypical Aeromonas salmonicida (aAs) vapA type V was the predominant bacterial species (70/215 bacterial isolates, 32.5% of bacterial samples; 43/117 positive individual fish, 36.8%) isolated in this survey followed by Vibrio species, which were the most geographically prevalent bacteria. Photobacterium indicum/profundum was also isolated from L. bergylta for the first time during this study. The collection of these bacterial isolates provides useful information for disease management. Identifying the aAs isolates involved in disease in ballan wrasse could provide vital information for improving/updating existing autogenous vaccines.

Bacterial adhesion to stainless steel is reduced by aqueous fish extract coatings

Biofilms ◽  
2006 ◽  
Vol 3 (1) ◽  
pp. 25-36 ◽  
Author(s):  
N. Bernbom ◽  
R. L. Jørgensen ◽  
Y. Y. Ng ◽  
R. L. Meyer ◽  
P. Kingshott ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Photoelectron Spectroscopy ◽  
Bacterial Adhesion ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Bacterial Attachment ◽  
Stainless Steel Surface ◽  
Flight Mass Spectrometry

ABSTRACTMicrobial adhesion and biofilm formation on surfaces pose major problems and risks to human health. One way to circumvent this problem is to coat surfaces (in this report stainless steel) with a non-toxic fish extract that generates an abiotic surface with less bacterial attachment than uncoated surfaces or surfaces coated with, for example, tryptone soy broth. The bacteria grow well in the fish extract; hence a general bacteriocidal effect is not the reason for the antifouling effect. Bacterial attachment was quantified by different methods including (a) direct fluorescence microscopy, (b) removal by ultrasound and subsequent quantification of the adhered bacteria, and (c) regrowth of the adhered bacteria measured by indirect conductometry. Surprisingly, the bacterial counts on surfaces coated with aqueous fish extract were 10–100 times lower than on surfaces coated with laboratory broths when surfaces were submerged in bacterial suspensions. The effect was seen forPseudomonas fluorescensAH2,Pseudomonas aeruginosaPAO1,Escherichia coliMG1655,Vibrio anguillarum90-11-287 andAeromonas salmonicidaJno 3175/88. It lasted for at least 7 days. Atomic force microscopy showed that steel surfaces conditioned with fish extract were covered by a thin layer of spherical, nanosized particles. Chemical analysis of the surfaces coated with adsorbed fish extract using X-ray photoelectron spectroscopy revealed that the layer was proteinaceous and had a thickness less than 2 nm. Numerous protein bands/peaks were also detected by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry techniques. We conclude that coating the stainless steel surface with fish extract results in a thin protein layer that reduces bacterial adhesion significantly.

Comparison of antibody responses in Atlantic cod (Gadus morhua L.) to Vibrio anguillarum, Aeromonas salmonicida and Francisella sp.

2009 ◽  
Vol 27 (2) ◽  
pp. 112-119 ◽  
Author(s):  
Merete Bjørgan Schrøder ◽  
Terje Ellingsen ◽  
Helene Mikkelsen ◽  
Edel Anne Norderhus ◽  
Vera Lund
Keyword(s):  
Gadus Morhua ◽  
Atlantic Cod ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Antibody Responses
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