Grape seeds from five known red grape sorts and two new local sorts, obtained by hybridization of red and white varieties, have been valorized as vegetal oils, through good yield extraction. The fatty acid content the of obtained oils was determined by GC-FID, as methyl esters, and directly by 1H-NMR spectroscopy. The obtained grape seed oils have a high content in linoleic acid (over 70%) and a relatively low quantity of saturated fatty acid (stearic and palmitic). Both measurement methods gave similar results confirming the accuracy of the spectroscopic method, which is less time and reagent consuming. Based on the fatty acid profile (FAP) of the oils, determined by either FT-IR or 1H-NMR and using the Principal Component Analysis (PCA) and Hierarchical Clustering Analysis (HCA) statistical methods, the discrimination between the classical known grape sorts and the new local varieties could be achieved. The results show that such method is appropriate for discovering possible adulteration of the grape oils, valuable products due to the essential unsaturated acid content.