Multiobjective optimization of Phoenix dactylifera L. seeds extraction: Mixture design methodology for phytochemical contents and antibacterial activity

2020 ◽  
Vol 44 (10) ◽  
Author(s):  
Slim Smaoui ◽  
Hajer Ben Hlima ◽  
Mariam Fourati ◽  
Khaoula Elhadef ◽  
Karim Ennouri ◽  
...  
Author(s):  
Lidia Ait Ouahioune ◽  
Fatma Bara ◽  
Karim Bariz ◽  
Karim Houali ◽  
Djamel Djenane

Background: Conventional food additives were associated with potential harm. Palm seeds are an interesting source of high-added value bioactive compounds, regarding their potential use in food industries. Aim: Date seeds extract (DSE) of Algerian Phoenix dactyliefra L. was studied for its phytochemical, antioxidant, and antibacterial activity and to identify its potential uses in foods. Material and methods: A total soluble solid of DSE (°Brix) was determined by refractometry. The total phenolic contents (TPCs) were determined according to the Folin-Cioclateu method and the total flavonoid and flavonols were also estimated with the colorimetric method. The DPPH method was used to determine the antioxidant activity of DSE. The IC50 values were also calculated and the antioxidant activity index (AAI) was determined. The agar well diffusion method was employed for the antibacterial activity of the aqueous DSE against various pathogens. Results: Moisture, ash, and fat contents (%) determined were 11.06, 1.33, and 7.06, respectively. The physicochemical analysis of the extract showed a pH value of 4.67, titratable acidity value of 0.64 citric acid/100 g, °Brix value = 3%, sugars values of 0.12, 0.47, and 0.35 g/L for reducing sugar, total sugar, and sucrose, respectively. The determination of phenolic compounds was carried out on three groups, namely total phenols = 229.67 mg GAEq/g, flavonoids = 201.12 mg QEq/g, and flavonols = 173.03 mg QEq/g, respectively. The DSE demonstrates to be effective as an antioxidant “in vitro” and showed a strong antibacterial activity with different inhibition levels, depending on the bacterial strains. Conclusion: The current investigations present knowledge about the possible role of palm industry by-products as a novel perspective for the development of new foods. We suggest that date seeds can be exploited in some food applications utilizing their high levels of bioactive molecules. Keywords: Phoenix dactylifera L., Degla-Baïda, seeds extract, bioactive molecules, biological activity, in vitro, novel perspective.


Bio-Research ◽  
2020 ◽  
Vol 18 (2) ◽  
Author(s):  
MR Adedayo ◽  
AE Ajiboye ◽  
FE Adetula

Dental diseases refer to conditions of deterioration and disintegration of the oral cavity due to the activities of pathogenic microbes. This study was carried out to determine the antibacterial activity of aqueous and ethanol extracts of Phoenix dactylifera L. (Date palm) seed on selected bacteria isolates associated with dental caries namely Staphylococcus aureus, Streptococcus mutans and Lactobacillus casei. Bioactive components of the seed were extracted using water and ethanol as solvents. The antibacterial activity of the extracts was examined by agar well diffusion method. The minimum inhibitory and bactericidal concentrations (MIC and MBC) were also determined using standard methods. All extracts were active against the tested isolates at the concentrations examined. L. casei was the most susceptible organism followed by S. mutans. S. aureus was the least susceptible. Ethanolic extract was more effective than aqueous extract at all the concentrations tested in the study. The MIC of aqueous and ethanolic extracts was 20 mg/ml while the MBC was 80 mg/ml respectively against all the bacterial isolates tested. It can be concluded that the aqueous and ethanolic extracts of the seed possess antibacterial potential against all the test organisms and could be useful in treatment of dental diseases and other related diseases caused by the bacteria isolates tested in the study.


2020 ◽  
Vol 1 (1) ◽  
pp. 52-59 ◽  
Author(s):  
Sameer Mohammed Yaseen ◽  
Hussein Adil Abid ◽  
Anwar Ali Kadhim ◽  
Entesar Emhamed Aboglida

This study aimed to assess the antibacterial activity of palm heart (Phoenix dactylifera L.) extract in vitro. Fresh material of the palm heart was dried, crushed and then extracted with distilled water, methanol and acetone. Distilled water extract was divided into crude, residues (after filtration), protein and non-protein parts. All extracts were investigated to determine their inhibitory activity against Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Streptococcus mutans (S. mutans), Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Proteus mirabilis (P. mirabilis), Enterobacter aerogenes (E. aerogenes), Morganella morganii (M. morganii), and Pseudomonas aeruginosa (P. aeruginosa) using agar well diffusion method, ceftriaxone (CTR) was used as a positive control. Phytochemical analyses were also performed to confirm the presence/absence of bioactive constituents. All extracts revealed antibacterial activity against some of the tested bacteria. When comparing the findings, aqueous (watery) extracts had the slightest antibacterial activity while the highest activity was related to acetone extraction. For Gram-positive bacteria, the highest activity of acetone extract was against S. mutans (10.7±0.13 mm) followed by S. pyogenes (10.6±0.17 mm), while Gram-negative most sensitive strain were E. aerogenes (15.3±0.20 mm) and P. mirabilis (14.4±0.48 mm) respectively. Phytochemical analyses have revealed the presence of steroids, alkaloids, pseudotannins, glycosides, carbohydrates, and aromatic amino acids. Finally, these findings indicate the activity of the heart of palm to fight germs, further studies may also be needed


Author(s):  
Lidia Ait Ouahioune ◽  
Fatma Bara ◽  
Karim Bariz ◽  
Karim Houali ◽  
Djamel Djenane

Background: Conventional food additives were associated with potential harm. Palm seeds are an interesting source of high-added value bioactive compounds, regarding their potential use in food industries. Aim: Date seeds extract (DSE) of Algerian Phoenix dactyliefra L. was studied for its phytochemical, antioxidant, and antibacterial activity and to identify its potential uses in foods. Material and methods: A total soluble solid of DSE (°Brix) was determined by refractometry. The total phenolic contents (TPCs) were determined according to the Folin-Cioclateu method and the total flavonoid and flavonols were also estimated with colorimetric method. The DPPH method was used to determine the antioxidant activity of DSE. The IC50 values were also calculated and the antioxidant activity index (AAI) was determined. The agar well diffusion method was employed for the antibacterial activity of the aqueous DSE against various pathogens. Results: Moisture, ash, and fat contents (%) determined were 11.06, 1.33, and 7.06, respectively. The physicochemical analysis of the extract showed a pH value of 4.67, titratable acidity value of 0.64 citric acid/100 g, °Brix value = 3%, sugars values of 0.12, 0.47, and 0.35 g/L for reducing sugar, total sugar, and sucrose, respectively. The determination of phenolic compounds was carried out on three groups, namely total phenols = 229.67 mg GAEq/g, flavonoids = 201.12 mg QEq/g, and flavonols = 173.03 mg QEq/g, respectively. The DSE demonstrates to be effective as an antioxidant “in vitro” and showed a strong antibacterial activity with different inhibition levels, depending on the bacterial strains. Conclusion: The current investigations present knowledge about the possible role of palm industry by-products as a novel perspective for the development of new foods. We suggest that date seeds can be exploited in some food applications utilizing their high levels of bioactive molecules. ​Keywords: Phoenix dactylifera L., Degla-Baïda, seeds extract, bioactive molecules, biological activity, in vitro, novel perspective.


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