Development of monoclonal antibodies for the rapid detection and identification of Salmonella enterica serovar Enteritidis in food sample using dot‐blot assays

2020 ◽  
Vol 40 (5) ◽  
Author(s):  
Chontichar Jinapon ◽  
Pradit Wangman ◽  
Chalinan Pengsuk ◽  
Parin Chaivisuthangkura ◽  
Paisarn Sithigorngul ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Salmonella Enterica ◽  
Rapid Detection ◽  
Food Sample ◽  
Dot Blot ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Detection And Identification

scholarly journals Rapid Detection of Salmonella enterica Serovar Choleraesuis in Blood Cultures by a Dot Blot Enzyme-Linked Immunosorbent Assay

2000 ◽  
Vol 7 (6) ◽  
pp. 977-979 ◽  
Author(s):  
Kritsana Janyapoon ◽  
Sunee Korbsrisate ◽  
Hatairat Thamapa ◽  
Sittichai Thongmin ◽  
Suwattana Kanjanahareutai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Salmonella Enterica ◽  
Rapid Detection ◽  
Direct Detection ◽  
Blood Cultures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Biochemical Method ◽  
Dot Blot

ABSTRACT A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella entericaserovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.

Incubation of Supplemented Egg Contents Pools To Support Rapid Detection of Salmonella enterica Serovar Enteritidis

2003 ◽  
Vol 66 (4) ◽  
pp. 656-659 ◽  
Author(s):  
RICHARD K. GAST ◽  
PETER S. HOLT
Keyword(s):  
Incubation Time ◽  
Salmonella Enterica ◽  
Rapid Detection ◽  
Salmonella Enteritidis ◽  
Incubation Temperature ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Enrichment Broth ◽  
Direct Plating ◽  
Internal Contamination ◽  
Rapid Methods

Detecting internal contamination of eggs with Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) is an important aspect of efforts to identify infected laying flocks. When egg contents pools are tested for Salmonella Enteritidis, a preliminary incubation step is often employed to allow small initial populations of contaminants to multiply to more easily detectable numbers. Consistent detection of Salmonella Enteritidis in egg pools by direct plating requires the presence of at least 105 CFU/ml, whereas some very rapid methods can require as many as 107 CFU/ml. The present study determined the rates at which initial inocula of approximately 10 Salmonella Enteritidis cells multiplied in 10-egg pools, some of which were supplemented with concentrated nonselective enrichment broth or with a source of iron. At 37°C, Salmonella Enteritidis concentrationsin supplemented egg pools usually reached 105 CFU/ml within 12 h and 107 CFU/ml by 12 to 15 h of incubation. At 25°C, Salmonella Enteritidis concentrations in supplemented egg pools typically attained 105 CFU/ml by 18 to 27 h and 107 CFU/ml by 27 to 36 h of incubation. At both temperatures, Salmonella Enteritidis multiplication was significantly slower in unsupplemented pools. Accordingly, the length of incubation time necessary for consistent detection of small numbers of Salmonella Enteritidis in egg contents pools depends on the incubation temperature used, on whether the egg pools are supplemented to increase the rate of bacterial multiplication, and on the sensitivity of subsequent tests applied to the incubated pools.

Optimization of a rapid dot-blot immunoassay for detection of Salmonella enterica serovar Enteritidis in poultry products and environmental samples

2004 ◽  
Vol 21 (6) ◽  
pp. 761-769 ◽  
Author(s):  
Ziad W Jaradat ◽  
Joanna H Bzikot ◽  
Jerzy Zawistowski ◽  
Arun K Bhunia
Keyword(s):  
Salmonella Enterica ◽  
Environmental Samples ◽  
Dot Blot ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Poultry Products

Rapid detection and identification of two lineages of influenza B strains with monoclonal antibodies

1999 ◽  
Vol 79 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Naoko Nakagawa ◽  
Akiko Maeda ◽  
Tetsuo Kase ◽  
Ritsuko Kubota ◽  
Yoshinobu Okuno
Keyword(s):  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Influenza B ◽  
Detection And Identification

scholarly journals Application of Rapid Dot Blot Immunoassay for Detection of Salmonella enterica Serovar Enteritidis in Eggs, Poultry, and Other Foods

2001 ◽  
Vol 67 (1) ◽  
pp. 459-461 ◽  
Author(s):  
Mark Akira Yoshimasu ◽  
Jerzy Zawistowski
Keyword(s):  
Monoclonal Antibody ◽  
Salmonella Enterica ◽  
Dot Blot ◽  
Salmonella Enterica Serovar Enteritidis

ABSTRACT Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

Evaluation of Whole Genome Sequencing for outbreak detection of Salmonella enterica serovar Enteritidis

2020 ◽  
Author(s):  
Ainhoa Arrieta-Gisasola ◽  
Aitor Atxaerandio Landa ◽  
Javier Garaizar ◽  
Joseba Bikandi ◽  
José Karkamo ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Salmonella Enterica ◽  
Outbreak Detection ◽  
Whole Genome ◽  
Salmonella Enterica Serovar Enteritidis
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