Solvent fractions of fermented
            Trapa japonica
            fruit extract stimulate collagen synthesis through TGF‐β1/GSK‐3β/β‐catenin pathway in human dermal fibroblasts

We have previously shown that Trapa japonica fruit extract (TJE) as well as its fermented extract (FTJ) can be potentially used to treat alopecia. In the current study, a newly synthesized peptide (PEP) was detected in an active compound isolated from FTJ. Several biological assays were conducted to verify the antiaging effects of TJE, FTJ, and PEP on the skin. We examined the effects of TJE, FTJ, and PEP on cell viability, collagen synthesis, and inhibition of mRNA expression of matrix metalloproteinases (MMPs), induced by tumor necrosis factor alpha (TNF-α), in human dermal fibroblasts (HDFs). In addition, a wound-healing assay of the human keratinocyte cell line (HaCaT) and a clinical study of antiaging activity were conducted. The findings confirmed that PEP exerted an effect on cell proliferation in a dose-dependent manner. Treatment with TJE, FTJ, and PEP increased collagen synthesis but inhibited TNF-α-induced mRNA expression of MMPs. Compared with TJE and FTJ, PEP promoted a significant level of wound recovery in HaCaT cells and also exhibited antiaging effect, as demonstrated by a clinical study. These results suggest that PEP shows potential as a skin antiaging cosmetic product.

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Nanofiber scaffolds attenuate collagen synthesis of human dermal fibroblasts through TGF-β1/TSG-6 pathway

Journal of Physics Materials ◽

10.1088/2515-7639/ab2563 ◽

2019 ◽

Vol 2 (4) ◽

pp. 044001 ◽

Cited By ~ 2

Author(s):

Bei Xie ◽

Wanzong Zhu ◽

Pinghui Ding ◽

Mengting Chen ◽

Ji Li ◽

...

Keyword(s):

Collagen Synthesis ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Nanofiber Scaffolds ◽

Tgf Β1

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CircAMD1 regulates proliferation and collagen synthesis via sponging miR-27a-3p in P63-mutant human dermal fibroblasts

Differentiation ◽

10.1016/j.diff.2021.04.002 ◽

2021 ◽

Author(s):

Pengjun Su ◽

Qi Qiao ◽

Gengfeng Ji ◽

Zhibo Zhang

Keyword(s):

Collagen Synthesis ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts

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Asiaticoside induces cell proliferation and collagen synthesis in human dermal fibroblasts

Zinc supplementation improves heme biosynthesis in rats exposed to lead ◽

10.18051/univmed.2015.v34.96-103 ◽

2015 ◽

Vol 34 (2) ◽

pp. 96

Author(s):

Linda Yuliati ◽

Etik Mardliyati ◽

Kusmarinah Bramono ◽

Hans Joachim Freisleben

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Retinoic Acid ◽

Collagen Synthesis ◽

Active Agent ◽

Dermal Fibroblasts ◽

Type Iii Collagen ◽

Type I ◽

Human Dermal Fibroblasts ◽

Type Iii

Background Asiatiocoside, a saponin component isolated from Centella asiatica can improve wound healing by promoting the proliferation of human dermal fibroblasts (HDF) and synthesis of collagen. The skin-renewing cells and type I and III collagen synthesis decrease with aging, resulting in the reduction of skin elasticity and delayed wound healing. Usage of natural active compounds from plants in wound healing should be evaluated and compared to retinoic acid as an active agent that regulates wound healing. The aim of this study was to compare and evaluate the effect of asiaticoside and retinoic acid to induce greater cell proliferation and type I and III collagen synthesis in human dermal fibroblast. Methods Laboratory experiments were conducted using human dermal fibroblasts (HDF) isolated from human foreskin explants. Seven passages of HDF were treated with asiaticoside and retinoic acid at several doses and incubated for 24 and 48 hours. Cell viability in all groups was tested with the MTT assay to assess HDF proliferation. Type I and III collagen synthesis was examined using the respective ELISA kits. Analysis of variance was performed to compare the treatment groups. Results Asiaticoside had significantly stronger effects on HDF proliferation than retinoic acid (p<0.05). The type III collagen production was significantly greater induction with asiaticoside compared to retinoic acid (p<0.05). Conclusion Asiaticoside induces HDF proliferation and type I and III collagen synthesis in a time- and dose-dependent pattern. Asiaticoside has a similar effect as retinoic acid on type I and type III collagen synthesis.

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