Race Differentiation in Fusarium oxysporum f.sp. chrysanthemi

2013 ◽  
Vol 161 (10) ◽  
pp. 675-688 ◽  
Author(s):  
Marco Troisi ◽  
Domenico Bertetti ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Race Differentiation

scholarly journals Marker Development for Differentiation of Fusarium oxysporum f. sp. Niveum Race 3 from Races 1 and 2

2021 ◽  
Vol 22 (2) ◽  
pp. 822
Author(s):  
Owen Hudson ◽  
Sumyya Waliullah ◽  
James C. Fulton ◽  
Pingsheng Ji ◽  
Nicholas S. Dufault ◽  
...  
Keyword(s):  
South Carolina ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Conventional Polymerase Chain Reaction ◽  
Detection Methods ◽  
Limiting Factors ◽  
Pcr Marker ◽  
Primer Sets ◽  
Race Differentiation ◽  
Race 2

Fusarium wilt of watermelon, caused by Fusarium oxysporum f. sp. niveum (FON), is pathogenic only to watermelon and has become one of the main limiting factors in watermelon production internationally. Detection methods for this pathogen are limited, with few published molecular assays available to differentiate FON from other formae speciales of F. oxysporum. FON has four known races that vary in virulence but are difficult and costly to differentiate using traditional inoculation methods and only race 2 can be differentiated molecularly. In this study, genomic and chromosomal comparisons facilitated the development of a conventional polymerase chain reaction (PCR) assay that could differentiate race 3 from races 1 and 2, and by using two other published PCR markers in unison with the new marker, the three races could be differentiated. The new PCR marker, FNR3-F/FNR3-R, amplified a 511 bp region on the “pathogenicity chromosome” of the FON genome that is absent in race 3. FNR3-F/FNR3-R detected genomic DNA down to 2.0 pg/µL. This marker, along with two previously published FON markers, was successfully applied to test over 160 pathogenic FON isolates from Florida, Georgia, and South Carolina. Together, these three FON primer sets worked well for differentiating races 1, 2, and 3 of FON. For each marker, a greater proportion (60 to 90%) of molecular results agreed with the traditional bioassay method of race differentiation compared to those that did not. The new PCR marker should be useful to differentiate FON races and improve Fusarium wilt research.

Race Differentiation in Fusarium oxysporum f. sp. carthami

1970 ◽  
Vol 60 (11) ◽  
pp. 1706 ◽  
Author(s):  
J. M. Klisiewicz
Keyword(s):  
Fusarium Oxysporum ◽  
Race Differentiation

scholarly journals Draft Genome Sequences of Three Fusarium oxysporum f. sp. niveum Isolates Used in Designing Markers for Race Differentiation

2020 ◽  
Vol 9 (42) ◽  
Author(s):  
Owen Hudson ◽  
Dylan Hudson ◽  
Pingsheng Ji ◽  
M. Emran Ali
Keyword(s):  
Fusarium Oxysporum ◽  
Draft Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Race Differentiation

ABSTRACT Here, we report the draft genome sequences of three Fusarium oxysporum f. sp. niveum isolates that were used to design markers for molecular race differentiation. The isolates were collected from watermelon fields in Georgia (USA) and were determined to be different races of F. oxysporum f. sp. niveum using a traditional bioassay.

Chemical and biological profiling on rhizobacteria from Physalis peruviana with antagonistic activity agaisnt Fusarium oxysporum

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
C Soto ◽  
J Riaño ◽  
P Jiménez ◽  
E Coy-Barrera
Keyword(s):  
Fusarium Oxysporum ◽  
Antagonistic Activity ◽  
Physalis Peruviana

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

Inhabitation of Antagonistic Bacterium B96-II to Fusarium oxysporum f. sp. asparagi*

2010 ◽  
Vol 2009 (6) ◽  
pp. 861-865 ◽  
Author(s):  
Liping MA ◽  
Bianqing HAO ◽  
Jing WANG ◽  
Shu QIN ◽  
Xiongwu QIAO
Keyword(s):  
Fusarium Oxysporum ◽  
Antagonistic Bacterium

Effects of Fusarium oxysporum f. sp. cubense race 4 on microorganisms and enzyme activities in the rhizosphere soil of banana

2013 ◽  
Vol 38 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Yong-hong HUANG ◽  
Shun LÜ ◽  
Chun-yu LI ◽  
Yue-rong WEI ◽  
Gan-jun YI
Keyword(s):  
Fusarium Oxysporum ◽  
Enzyme Activities ◽  
Rhizosphere Soil ◽  
Race 4

Inheritance of Resistance to Isolates of Fusarium oxysporum var. batatas in Burley Tobacco 1 , 3

Crop Science ◽  
1972 ◽  
Vol 12 (4) ◽  
pp. 522-525 ◽  
Author(s):  
J. L. Jones ◽  
C. L. Gupton ◽  
T. R. Terrill
Keyword(s):  
Fusarium Oxysporum ◽  
Inheritance Of Resistance ◽  
Burley Tobacco
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