Chemical and biological profiling on rhizobacteria from Physalis peruviana with antagonistic activity agaisnt Fusarium oxysporum

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
C Soto ◽  
J Riaño ◽  
P Jiménez ◽  
E Coy-Barrera
Keyword(s):  
Fusarium Oxysporum ◽  
Antagonistic Activity ◽  
Physalis Peruviana

Effects of fengycins and iturins on Fusarium oxysporum f. sp. physali and root colonization by Bacillus velezensis Bs006 protect golden berry against vascular wilt

2021 ◽  
Author(s):  
Carlos Andrés Moreno-Velandia ◽  
Marc Ongena ◽  
Alba Marina Cotes
Keyword(s):  
Fusarium Oxysporum ◽  
Liquid Culture ◽  
Inhibition Zone ◽  
Root Surface ◽  
Antagonistic Activity ◽  
Dependent Manner ◽  
Bacillus Velezensis ◽  
Concentration Dependent Manner ◽  
Physalis Peruviana ◽  
Real Effects

Bacillus velezensis Bs006 has shown antagonistic activity on Fusarium oxysporum f. sp. physali (Foph) and biocontrol activity against Fusarium wilt (FW) in golden berry (Physalis peruviana). We hypothesized that strain Bs006 has the ability to synthesize antimicrobial cyclic lipopeptides (CLPs) like other members of the same species. However, if so, the real effects of CLPs on Foph and their potential as a biocontrol tool against Physalis-FW had not been elucidated. In this study the CLPs profile of Bs006 in liquid culture, and antagonist-plant-pathogen interactions were characterized. Also, the potential effects of supernatant free of bacteria against Foph and FW were explored and compared to the effects of pure CLPs. UPLC-ESI-MS analysis revealed the capacity of Bs006 to synthesize homologous compounds of iturins, surfactins and fengycins in liquid culture as well as on the inhibition zone against Foph in dual confrontation tests. Bs006-supernatant reduced the germination and growth of Foph and caused vacuolization, swelling and lysis of Foph cells in a concentration-dependent manner. Pure fengycins affected the development of Foph from 11 mg L-1 while iturins from 21 mg L-1. In a gnotobiotic system, Bs006 colonized the root surface of golden berry, inhibited the growth of Foph, and produced CLPs. Individual application of Bs006 and supernatant protected the plants from Foph infections by 37 and 53%, respectively. Meanwhile, fengycins reduced the disease progress by 39%. These results suggest further studies to select an optimum combination of Bs006 and supernatant or CLPs which might be a good option as biofungicide against Foph.

scholarly journals Antagonistic Activity of Pseudomonas Fluorescens Against Fusarium Oxysporum f. sp. Nievum Isolated from Soil Samples in Palestine

2017 ◽  
Vol 6 (2) ◽  
pp. 1 ◽  
Author(s):  
Mazen Salman ◽  
Nabil Shahin ◽  
Nawaf Abu-Khalaf ◽  
Mohammad Jawabrih ◽  
Basima Abu Rumaileh ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Field Experiments ◽  
Antagonistic Activity ◽  
Arab Countries ◽  
Local Market ◽  
Control Mechanisms ◽  
The Past ◽  
Normal Seedling ◽  
Severe Infections

Watermelon is an important summer crop in Palestine, for several decades filling the needs of local market and some Arab countries. The yield of watermelon decreased dramatically in recent years due to severe infections with the soil borne fungal pathogen Fusarium oxysporum f. sp. niveum (FON). Soil fumigation with methyl bromide was commonly applied by Palestinian farmers until it was recently legally banned. Different control mechanisms were not feasible to overcome problems caused by the disease resulting in decreased watermelon cultivation in Palestine for the past 30 years. In this work, we have experimentally shown that Pseudomonas fluorescens was efficient in controlling FON infection and allowing normal seedling growth of both the root and shoot systems. Field experiments are necessary to further confirm the efficacy of biocontrol application.

scholarly journals Identification of Immunity Related Genes to Study the Physalis peruviana – Fusarium oxysporum Pathosystem

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e68500 ◽  
Author(s):  
Felix E. Enciso-Rodríguez ◽  
Carolina González ◽  
Edwin A. Rodríguez ◽  
Camilo E. López ◽  
David Landsman ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Physalis Peruviana

scholarly journals Potential of non-pathogenic Fusarium oxysporum isolates for control of Fusarium wilt of tomato

2005 ◽  
Vol 30 (4) ◽  
pp. 409-412 ◽  
Author(s):  
Juliano C. da Silva ◽  
Wagner Bettiol
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Antagonistic Activity ◽  
Healthy Plant ◽  
Conidial Suspension ◽  
Vascular Wilt ◽  
Tomato Seedling ◽  
Seedling Roots ◽  
Race 2 ◽  
Cultivation Substrate

This study was done to evaluate the efficiency of non-pathogenic Fusarium oxysporum isolates (141/3, 233, 233/1, 245, 245/1, 251, 251/2, 251/5, and 257) in controlling vascular wilt caused by F. oxysporum f. sp. lycopersici, race 2 (isolates C-21A, TO11, and TO245) in tomato (Lycopersicon esculentum) cv. Viradoro seedlings. In order to determine the effect of non-pathogenic F. oxysporum isolates in tomato plants, the root system of 30-day-old seedlings was immersed in conidial suspensions (10(6) ml-1) of each isolate and the seedlings were transplanted to a cultivation substrate. Thirty-five days after transplanting it was observed that the non-pathogenic F. oxysporum isolates were not pathogenic to the cv. Viradoro nor did they affect seedling development. The efficiency of the non-pathogenic F. oxysporum isolates in controlling Fusarium wilt was determined by immersing the tomato seedling roots in the conidial suspension (10(6) ml-1) of each isolate and then transplanting them into substrates previously infested with isolates of F. oxysporum f.sp. lycopersici, race 2 (10(5) conidia ml-1 of substrate). Evaluations were performed 35 days after transplanting, for severity in scale with 1=healthy plant to 6=dead plant or plant showing vessel browning and wilted leaves up to the leader shoot and seedling height. The non-pathogenic F. oxysporum isolates were efficient in reducing the severity of the disease and maintaining normal plant development. These results provide evidence of the antagonistic activity of non-pathogenic F. oxysporum isolates in controlling vascular wilt caused by F. oxysporum f. sp. lycopersici race 2 in tomato.

scholarly journals Recovery of Fusarium oxysporum Fo47 Mutants Affected in Their Biocontrol Activity After Transposition of the Fot1 Element

2002 ◽  
Vol 92 (9) ◽  
pp. 936-945 ◽  
Author(s):  
Sophie Trouvelot ◽  
Chantal Olivain ◽  
Ghislaine Recorbet ◽  
Quirico Migheli ◽  
Claude Alabouvette
Keyword(s):  
Fusarium Oxysporum ◽  
Insertional Mutagenesis ◽  
Wild Type Strain ◽  
Growth Habit ◽  
Antagonistic Activity ◽  
Phenotypic Characterization ◽  
Wild Type ◽  
Biocontrol Activity

To investigate the biocontrol mechanisms by which the antagonistic Fusarium oxysporum strain Fo47 is active against Fusarium wilt, a Fot1 transposon-mediated insertional mutagenesis approach was adopted to generate mutants affected in their antagonistic activity. Ninety strains in which an active Fot1 copy had transposed were identified with a phenotypic assay for excision and tested for their biocontrol activity against F. oxysporum f. sp. lini on flax in greenhouse experiments. Sixteen strains were affected in their capacity to protect flax plants, either positively (more antagonistic than Fo47) or negatively (less antagonistic). The molecular characterization of these mutants confirms the excision of Fot1 and its reinsertion in most of the cases. Moreover, we demonstrate that other transposable elements such as Fot2, impala, and Hop have no transposition activity in the mutant genomes. The phenotypic characterization of these mutants shows that they are affected neither in their in vitro growth habit nor in their competitiveness in soil compared with wild-type strain Fo47. These results show that mutants are not impaired in their saprophytic phase and suggest that the altered biocontrol phenotype should likely be expressed during the interaction with the host plant.

scholarly journals Antagonistic Activity and Characterization of Indigenous Soil Isolates of Bacteria and Fungi Against Onion Wilt Incited by Fusarium sp

2021 ◽  
Author(s):  
Hilda Karim ◽  
Andi Asmawati ◽  
Oslan Jumadi
Keyword(s):  
Fusarium Oxysporum ◽  
Evolutionary Relationship ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Bacterial Isolates ◽  
Gene Markers ◽  
Culture Techniques ◽  
Fungal Isolates ◽  
Bacteria And Fungi

Abstract Tuber rot disease due to phytopathogen Fusarium oxysporum f. sp. cepae (Foc) infection is one of the main factors causing the decreasing amount of global shallot production. This study aims to find bacteria and fungi candidates which have Foc antagonistic activity through in vitro tests using dual culture techniques. A total of five bacterial isolates and three fungal isolates isolated from the rhizosphere of healthy onion plants showed the ability to inhibit Foc growth. B1 and B4 bacterial isolates had an average inhibitory capability of 65.93% and 72.27% respectively. Whereas C1 and C2 fungal isolates have the ability to inhibit the growth of Foc by as much as 74.82% and 67.76% respectively. The four tested microbial isolates were able to significantly inhibit Foc activity in vitro based on the ANOVA test, with values α = 0.05, and n = 3. Molecular analysis based on 16S-rRNA markers showed bacterial isolates B1 and B4 have an evolutionary relationship with B. subtilis. Whereas fungi C1 and C2 have evolutionary relationships with Aspergillus tubingensis and Trichoderma asperellum respectively, based on internal transcribed spacer (ITS) gene markers. The results of this study can be used to develop indigenous microbial consortiums as biological control agents for phytopathogenic fungi Fusarium oxysporum f. sp. cepae (Foc) on shallots.

scholarly journals Yield and physicochemical quality of Physalis peruviana L. fruit related to the resistance response against Fusarium oxysporum f. sp. physali

2019 ◽  
Vol 37 (2) ◽  
pp. 120-128
Author(s):  
Franklin Mayorga-Cubillos ◽  
Jorge Argüelles-Cárdenas ◽  
Edwin Rodríguez-Velásquez ◽  
Carolina González-Almario ◽  
Claudia Ariza-Nieto ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Crop Improvement ◽  
Physicochemical Characterization ◽  
Principal Component ◽  
Differential Resistance ◽  
Resistance Response ◽  
Physalis Peruviana ◽  
Fruit Cracking ◽  
Medicinal Value ◽  
Cape Gooseberry

Cape gooseberry (Physalis peruviana L.) is a fruit of great interest, due to its high nutritional and potential medicinal value. Vascular wilt disease caused by the fungus Fusarium oxysporum f. sp. Physali (Foph) is responsible for crop losses of up to 100% which makes necessary to identify resistant cultivars. To contribute to crop improvement processes, a physicochemical characterization was performed on fruits of 33 cape gooseberry genotypes using 18 quantitative descriptors. The genotypes were planted in the field under high and no pressure of Foph. The Student’s t test detected statistically significant differences (P<0.05) between the two conditions for yield, fruit cracking (%) and fruit juice pH. The principal component analysis explained in five factors 84.96% of the total variance, in which the fruit physical variables were the major contributor to the first component (41.65%). Cluster analysis grouped the genotypes under high and no pressure in seven and eight clusters, respectively. Two contrasting genotypesshowing differential resistance response to the pathogen wereanalyzed for fruit antioxidant capacity, in which DPPH and ORAC methods presented significant differences (P<0.05) between the two genotypes with greater antioxidant activity in the susceptible material.

scholarly journals Weed-Associated Fungal Endophytes as Biocontrol Agents of Fusariumoxysporum f. sp. cubense TR4 in Cavendish Banana

2021 ◽  
Vol 7 (3) ◽  
pp. 224
Author(s):  
Dennice G. Catambacan ◽  
Christian Joseph R. Cumagun
Keyword(s):  
Fusarium Oxysporum ◽  
Inhibitory Activity ◽  
Disease Incidence ◽  
Fungal Endophytes ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Trichoderma Asperellum ◽  
Cavendish Banana ◽  
Leaf Yellowing ◽  
Dual Culture Assay

The antagonistic activity of fungal endophytes isolated from weeds growing in Cavendish banana farms was determined against Fusarium oxysporum f. sp. cubense TR4 (Foc TR4) causing Fusarium wilt of Cavendish banana. Forty-nine out of the total 357 fungal endophytes from the roots of weeds exhibited antagonistic activity against Foc TR4. High inhibitory activity at 79.61–99.31% based on dual culture assay was recorded in endophytes Lasiodiplodia theobromae TDC029, Trichoderma asperellum TDC075, Ceratobasidium sp. TDC037, Ceratobasidium sp. TDC241, and Ceratobasidium sp. TDC474. All five endophytes were identified through DNA sequencing with 86–100% identity. Endophyte-treated Grand Naine and GCTCV 218 plantlets showed significantly lower disease incidence (p = 0.014), significantly lower degree of leaf yellowing (p = 0.037) and rhizome discoloration (p = 0.003). In addition, the cultivar Grand Naine was consistently highly susceptible compared with the tolerant cultivar GCTCV 218.

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