Danshen (S alvia miltiorrhiza ) water extract inhibits paracetamol-induced toxicity in primary rat hepatocytes via reducing CYP2E1 activity and oxidative stress

2015 ◽  
Vol 67 (7) ◽  
pp. 980-989 ◽  
Author(s):  
Xuelin Zhou ◽  
Ching Mei Cheung ◽  
Jia-ming Yang ◽  
Penelope M.Y. Or ◽  
Wayne Y.W. Lee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Water Extract ◽  
Rat Hepatocytes ◽  
Primary Rat Hepatocytes ◽  
Cyp2e1 Activity ◽  
And Oxidative Stress

Protein Synthesis Inhibition and Oxidative Stress Induced by Cylindrospermopsin Elicit Apoptosis in Primary Rat Hepatocytes

2013 ◽  
Vol 26 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Henar López-Alonso ◽  
Juan Andrés Rubiolo ◽  
Félix Vega ◽  
Mercedes R. Vieytes ◽  
Luis M. Botana
Keyword(s):  
Oxidative Stress ◽  
Protein Synthesis ◽  
Rat Hepatocytes ◽  
Protein Synthesis Inhibition ◽  
Synthesis Inhibition ◽  
Primary Rat Hepatocytes ◽  
And Oxidative Stress

scholarly journals Centella asiatica Attenuates Mitochondrial Dysfunction and Oxidative Stress in Aβ-Exposed Hippocampal Neurons

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Nora E. Gray ◽  
Jonathan A. Zweig ◽  
Donald G. Matthews ◽  
Maya Caruso ◽  
Joseph F. Quinn ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
Mitochondrial Function ◽  
Hippocampal Neurons ◽  
Neuroblastoma Cells ◽  
Water Extract ◽  
Centella Asiatica ◽  
Antioxidant Response ◽  
And Oxidative Stress

Centella asiatica has been used for centuries to enhance memory. We have previously shown that a water extract of Centella asiatica (CAW) protects against the deleterious effects of amyloid-β (Aβ) in neuroblastoma cells and attenuates Aβ-induced cognitive deficits in mice. Yet, the neuroprotective mechanism of CAW has yet to be thoroughly explored in neurons from these animals. This study investigates the effects of CAW on neuronal metabolism and oxidative stress in isolated Aβ-expressing neurons. Hippocampal neurons from amyloid precursor protein overexpressing Tg2576 mice and wild-type (WT) littermates were treated with CAW. In both genotypes, CAW increased the expression of antioxidant response genes which attenuated the Aβ-induced elevations in reactive oxygen species (ROS) and lipid peroxidation in Tg2576 neurons. CAW also improved mitochondrial function in both genotypes and increased the expression of electron transport chain enzymes and mitochondrial labeling, suggesting an increase in mitochondrial content. These data show that CAW protects against mitochondrial dysfunction and oxidative stress in Aβ-exposed hippocampal neurons which could contribute to the beneficial effects of the extract observed in vivo. Since CAW also improved mitochondrial function in the absence of Aβ, these results suggest a broader utility for other conditions where neuronal mitochondrial dysfunction occurs.

Quercetin protects rat hepatocytes from oxidative damage induced by ethanol and iron by maintaining intercellular liable iron pool

2013 ◽  
Vol 33 (5) ◽  
pp. 534-541 ◽  
Author(s):  
Y Li ◽  
Y Deng ◽  
Y Tang ◽  
H Yu ◽  
C Gao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Iron Overload ◽  
Combined Treatment ◽  
Rat Hepatocytes ◽  
Redox Status ◽  
Glutathione Depletion ◽  
Labile Iron Pool ◽  
Iron Pool ◽  
And Oxidative Stress

Accumulating evidence has shown that ethanol-induced iron overload plays a crucial role in the development and progression of alcoholic liver disease. We designed the present study to investigate the potential protective effect of quercetin, a naturally occurring iron-chelating antioxidant on alcoholic iron overload and oxidative stress. Ethanol-incubated (100 mmol/L) rat primary hepatocytes were co-treated by quercetin (100 µmol/L) and different dose of ferric nitrilotriacetate (Fe-NTA) for 24 h. When the hepatic enzyme releases in the culture medium, redox status of hepatocytes and the intercellular labile iron pool (LIP) level were assayed. Our data showed that Fe-NTA dose dependently induced cellular leakage of aspartate transaminase and lactate dehydrogenase, glutathione depletion, superoxide dismutase inactivation, and overproduction of malondialdehyde) and reactive oxygen species (ROS) of intact and especially ethanol-incubated hepatocytes. The oxidative damage resulted from ethanol, Fe-NTA, and especially their combined treatment was substantially alleviated by quercetin, accompanying the corresponding normalization of intercellular LIP level. Iron in excess, thus, may aggravate ethanol hepatotoxicity through Fenton-active LIP, and quercetin attenuated ethanol-induced iron and oxidative stress. To maintain intercellular LIP contributes to the hepatoprotective effect of quercetin besides its direct ROS-quenching activity.

scholarly journals Antioxidant and hepatoprotective effects of the food seasoning curry leaves Murraya koenigii (L.) Spreng. (Rutaceae)

RSC Advances ◽  
2015 ◽  
Vol 5 (122) ◽  
pp. 100589-100597 ◽  
Author(s):  
Wan Yong Ho ◽  
Boon Kee Beh ◽  
Kian Lam Lim ◽  
Nurul Elyani Mohamad ◽  
Hamidah Mohd Yusof ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gallic Acid ◽  
Liver Damage ◽  
Water Extract ◽  
Leaf Water ◽  
Murraya Koenigii ◽  
Curry Leaves ◽  
Hepatoprotective Effects ◽  
And Oxidative Stress

The curry leaf water extract, which is rich in gallic acid, reversed paracetamol-induced liver damage in mice by reducing inflammation and oxidative stress.

d-Amphetamine-induced cytotoxicity and oxidative stress in isolated rat hepatocytes

2011 ◽  
Vol 18 (4) ◽  
pp. 279-285 ◽  
Author(s):  
Osama S. El-Tawil ◽  
Ali H. Abou-Hadeed ◽  
Mohamed F. EL-Bab ◽  
Abeir A. Shalaby
Keyword(s):  
Oxidative Stress ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
And Oxidative Stress ◽  
Isolated Rat

scholarly journals Önemli Tıbbi Bitkilerden Biri Olan Achillea millefolium L. (Civanperçemi) Bitkisinin Besin Elementi Konsantrasyonları ve Antioksidan Aktivitesi

2021 ◽  
Vol 9 (3) ◽  
pp. 590-594
Author(s):  
Handan Saraç ◽  
Hasan Durukan ◽  
Ahmet Demirbaş
Keyword(s):  
Oxidative Stress ◽  
Water Extract ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Achillea Millefolium ◽  
Nutrient Concentrations ◽  
Oxidative Stress Index ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

In this study, it was aimed to determine the nutrient concentrations and antioxidant activity of Achillea millefolium L. (Yarrow), which is known to have medicinal properties. For this purpose, macro and micronutrient concentrations, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of water extract Yarrow plant, which is known to be collected from Divrigi district in Sivas province, were determined. The results have shown that the yarrow plant is sufficient in terms of some macro and micronutrient concentrations, and the nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium from macro elements, iron (Fe), zinc (Zn), manganese (Mn) and copper (Cu) from micro elements concentrations were determined as 1.01% N, 0.63% P, 2.43% K, 2.22% Ca, 0.70% Mg, 360.4 mg kg-1 Fe, 47.6 mg kg-1 Zn, 85.5 mg kg-1 Mn and 28.3 mg kg-1 Cu, respectively. It has been determined that its antioxidant potential is moderate. In addition, the plant’s oxidant capacity and oxidative stress index were found to be low.

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