Microbiological analysis of conjunctival secretion in anophthalmic cavity, contralateral eye and ocular prosthesis of patients with maxillofacial abnormalities

A.M. Guiotti; E.V.F. da Silva; I.A. Catanoze; K.H.T. de Carvalho; E.M. Malavazi; M.C. Goiato; D.M. dos Santos; M.T.G. de Almeida

doi:10.1111/lam.12830

Effectiveness of 0.66% Povidone-Iodine Eye Drops on Ocular Surface Flora before Cataract Surgery: A Nationwide Microbiological Study

Journal of Clinical Medicine ◽

10.3390/jcm10102198 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2198

Author(s):

Rosario Musumeci ◽

Pasquale Troiano ◽

Marianna Martinelli ◽

Matteo Piovella ◽

Claudio Carbonara ◽

...

Keyword(s):

Cataract Surgery ◽

Ocular Surface ◽

Povidone Iodine ◽

Bacterial Species ◽

Bacterial Flora ◽

Bacterial Load ◽

Controlled Study ◽

Eye Drops ◽

Microbiological Analysis ◽

Contralateral Eye

A multicenter, nonrandomized, prospective, controlled study was conducted to evaluate, as perioperative prophylactic treatment, the anti-infective effectiveness of 0.66% povidone-iodine eye drops (IODIM®) against the bacterial flora of the conjunctival surface of patients who undergo cataract surgery. Eye drops containing 0.66% povidone-iodine were applied to the eye undergoing cataract surgery; the untreated contralateral eye was used as control. One hundred and twenty patients set to receive unilateral cataract surgery were enrolled in 5 Italian Ophthalmology Centers and pretreated for three days with 0.66% povidone-iodine eye drops. The contralateral eye, used as control, was left untreated. Conjunctival swabs of both eyes were collected at the baseline visit and after three days of treatment, just before the cataract surgery. A qualitative and quantitative microbiological analysis of bacterial presence was evaluated by means of bacterial culture, followed by identification. Methicillin resistance determination was also performed on staphylococci isolates. Bacterial load before and after treatment of the eye candidate for cataract surgery was evaluated and compared to the untreated eye. A reduction or no regrowth on the culture media of the bacterial load was observed in 100% of the study subjects. A great heterogenicity of bacterial species was found. The 0.66% povidone-iodine eye drops, used for three days prior to cataract surgery, were effective in reducing the conjunctival bacterial load. The 0.66% povidone-iodine eye drops (IODIM®) might represent a valid perioperative prophylactic antiseptic adjuvant treatment to protect the ocular surface from microbial contamination in preparation of the surgical procedure.

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The influence of the locomotor stump’s form on the ocular prosthetics result with different methods of eye removal

Ophthalmology journal ◽

10.17816/ov13531 ◽

2020 ◽

Vol 13 (1) ◽

pp. 77-85

Author(s):

Nadezhda A. Baranova ◽

Irina A. Senina ◽

Vadim P. Nikolaenko

Keyword(s):

Frontal Plane ◽

Optimal Shape ◽

Back Surface ◽

Palpebral Fissure ◽

Ocular Prosthesis ◽

Orbital Prosthesis ◽

Examination Methods ◽

Contralateral Eye ◽

Upper Third ◽

Corneal Diameter

Aim. To determine the optimal shape of the locomotor stump and the configuration of the corresponding ocular prosthesis, ensuring their maximum motility in patients with anophthalmia with different methods of eye removal. Materials and methods. The study group consisted of 132 patients aged 1880 years after enucleation or evisceration. Examination methods included medical history; examination of eyelids, measurement of length and width of the palpebral fissure, as well as of the depth of conjunctival fornices on both sides; assessment of the volume, shape, surface topography, position and excursions of the locomotor stump, of the protrusion of the ocular prosthesis compared to the contralateral eye; photo registration of the studied parameters. Results. During the study, there were 3 types of locomotor stump identified: moderate with retraction in the upper third; voluminous flattened; voluminous hemispherical. The locomotor stump after enucleation was voluminous flattened or moderate with retraction in the upper third. The best motility of the locomotor stump was noted nasally and downward. The motility of the ocular prosthesis was 47.4% compared to the contralateral eye. The locomotor stump after evisceration with keratectomy was voluminous hemispherical or voluminous flattened. Its motility in all four directions was about the same. The motility of the ocular prosthesis in comparison to the contralateral eye was 55.9%. The locomotor stump after evisceration without keratectomy was voluminous hemispherical, uniform, smooth. The motility of the locomotor stump was maximal in comparison to other groups and relatively equal in all four directions. The motility of the ocular prosthesis in comparison to the contralateral eye was 68.2%. Conclusion. The optimal shape of the locomotor stump, providing the greatest motility of the ocular prosthesis is voluminous hemispherical. The same protrusion of the eyeball and that of the cosmetic prosthesis relatively to the frontal plane after enucleation is achieved by increasing the thickness of the prosthesis itself, which reduces its motility. Evisceration with implantation of the orbital prosthesis involves the use of a thin-walled ocular prosthesis, the back surface of which ideally repeats the locomotor stump surface and does not prevent its maximum motility. When removing a squinting eyeball with preserved corneal diameter, a smaller implant should be used to prevent excessive opening of the palpebral fissure, or to prefer evisceration with keratectomy.

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Custom-made Ocular Prosthesis for Rehabilitation of Missing Parts of the Face: A Case Report

Acta Medica Philippina ◽

10.47895/amp.v55i8.2119 ◽

2021 ◽

Vol 55 (8) ◽

Author(s):

Imam Safari Azhar ◽

Rizko Wira Artha Megantara ◽

Agus Dahlan

Keyword(s):

Congenital Defects ◽

Psychological Burden ◽

Ocular Prosthesis ◽

Maxillofacial Prosthesis ◽

Custom Made ◽

Dental Hospital ◽

The Face ◽

Eye Prosthesis ◽

Contralateral Eye ◽

The Right

Background. Eyes are an important component of the face and vital organs of vision. Eye loss can be caused by congenital defects, trauma, or tumor. Loss of an eye produces physical abnormalities that pose a psychological burden on the patient, as well as visual function damage. An ocular prosthesis is an artificial maxillofacial prosthesis to replace the lost eye. Case summary. A 54-year-old man consults at the Prosthodontics Specialist Universitas Airlangga Dental Hospital for an eye prosthesis. The patient did not have the right eyeball since birth. Soft tissue around the eye is normal, including the palpebral muscles. Case management. An impression was made using a custom tray and alginate in the defect area, followed by filling the impression with gypsum type 3 to get a working model, from which a wax model is made and adjusted to the patient. After that, sclera and ocular acrylic prostheses are made on the basis of an adjusted wax model. The prosthesis is then polished and colored according to the contralateral eye. Then the prosthesis is delivered to the patient. Conclusion. The hollow custom-made eye prosthesis can be considered in the treatment of anophthalmia. It is able to improve the patient's psychological and emotional status.

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Microbiological analysis of popular spreads used in restaurants inside Dhaka city, Bangladesh

Stamford Journal of Microbiology ◽

10.3329/sjm.v10i1.50727 ◽

2020 ◽

Vol 10 (1) ◽

pp. 16-19

Author(s):

Tasnia Ahmed ◽

Md Aftab Uddin

Keyword(s):

Raw Materials ◽

Peanut Butter ◽

Food Additives ◽

Microbiological Analysis ◽

Dhaka City ◽

Acceptable Result ◽

Fast Foods ◽

Public Consumption ◽

Viable Bacteria ◽

Almost All

Spreads are used widely for making the fast foods more amazing and tasty. Varieties and cross combinations of ingredients are used to make many flavours of fast foods. Different restaurants prepare their signature spreads for attracting people but this can cause opposite result if not prepared using high quality raw materials and proper hygienic conditions are not maintained. Current study was conducted on ten different types of spreads (pesto, cilantro, queso, tomato sauce, peanut butter, honey, mustard, cream cheese, chocolate sauce and butter) which are used by the local restaurants in Dhaka city, Bangladesh. Almost all the samples (nine out of ten) harbored total viable bacteria exceeding the standard limit. Four spread samples showed high fungal count (102 cfu/gm). Four samples showed to be positive (˃101/ml recommended acceptable count) for Escherichia coli and Klebsiella spp. which indicates that these spread cannot be recommended for public consumption. Among all the samples examined, only chocolate sauce showed acceptable result without the presence of coliforms, Staphylococcus spp. and Pseudomonas spp. This finding suggests proper maintenance of sanitation in spread preparation and selling area. A proper guidelines and monitoring can help keep up the quality of food additives. Stamford Journal of Microbiology, Vol.10 (1) 2020: 16-19

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Microbiological analysis of Lada Vegetable Production Units (VPU) to define critical control points and procedures to ensure the safety of space grown vegetables

40th International Conference on Environmental Systems ◽

10.2514/6.2010-6255 ◽

2010 ◽

Cited By ~ 4

Author(s):

Mary Hummerick ◽

Jay Garland ◽

Gail Bingham ◽

V Sychev ◽

I. Podolsky

Keyword(s):

Vegetable Production ◽

Microbiological Analysis ◽

Control Points ◽

Critical Control Points

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Use of a drug containing ornidazole-neomycin–prednisolone–econazole for the empirical treatment of vaginal infections

GYNECOLOGY ◽

10.26442/20795696.2020.4.200304 ◽

2020 ◽

Vol 22 (4) ◽

pp. 28-32

Author(s):

Valerii G. Volkov ◽

Tatyana V. Zakharova

Keyword(s):

Inflammatory Diseases ◽

Local Treatment ◽

Empirical Treatment ◽

New Combination ◽

Microbiological Analysis ◽

Vaginal Infections ◽

Aerobic Vaginitis ◽

Burning Pain ◽

Lower Genital Tract ◽

Unpleasant Smell

Relevance. Empirical treatment of vaginitis is indicated due to its polymicrobial etiology and limited microbiological analysis. The aim of the study was to study the combination of ornidazoleneomycinprednisoloneeconazole (ONPE, Elzhina) as a first-line drug for local treatment of patients with various forms of non-specific vaginitis. Materials and methods. The study included 55 non-pregnant women aged 18 to 50 years (381.5 years) who had clinical and laboratory signs of acute vaginitis. Results. All patients at the initial treatment complained of pathological discharge, 47 (85.5%) noted itching, burning, pain in the genital area of various degrees of severity, 17 (30.9%) discomfort when urinating, 9 (16.4%) unpleasant smell, 8 (14.5%) dyspareunia, 4 (2.2%) complained of sensations of a foreign body in the vagina. The clinical and laboratory effectiveness of treatment was 89.1% (49). In 6 (10.9%) patients, clinical improvement was noted, with the remaining laboratory signs of aerobic vaginitis. Conclusion. The new combination of ornidazoleneomycinprednisoloneeconazole (ONPE, Elzhina ) has a good efficacy and safety profile and can be used to initiate empirical treatment of non-specific inflammatory diseases of the lower genital tract.

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Isolation of Stenotrophomonas maltophilia from Clinical Samples in Some Hospitals in Shiraz, Iran

Clinical and Experimental Investigations ◽

10.31487/j.cei.2020.03.01 ◽

2020 ◽

pp. 1-3

Author(s):

Majid Baserisalehi ◽

Samira Zarezadeh ◽

Majid Baserisalehi ◽

Saeed Shoa

Keyword(s):

Stenotrophomonas Maltophilia ◽

Enterobacter Aerogenes ◽

Late Summer ◽

Early Spring ◽

Clinical Samples ◽

Bacillus Species ◽

Microbiological Analysis ◽

Blood Samples ◽

Healthcare Facilities ◽

Phenotypic Methods

Stenotrophomonas maltophilia is an emerging pathogenic non-fermentative Gram-negative Bacillus species. It has caused many nosocomial infections and can be isolated from various hospital wards and healthcare facilities. Research has shown that most of its strains are inherently resistant to many antibiotics and have multidrug resistance. This research intended to determine its occurrence frequency at some Hospitals in shiraz, Iran. The present study was conducted in six months (from early spring to late summer 2019). Clinical samples (Blood, Urine and cerebrospinal fluid (CSF)) collected from 120 patients afflicted with various infections. The samples were transferred to the Laboratory and subjected to microbiological analysis. Identification of the isolates was carried out by phenotypic methods and Stenotrophomonas maltophilia isolates verified using molecular methods. In total, various bacteria were isolated from 84 clinical samples. The isolates were Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Staphylococcus aureus and Pseudomonas aeruginosa. Stenotrophomonas maltophilia was isolated from 17 (20.2%) positive samples and most of them were isolated from blood samples. Our finding indicated that Stenotrophomonas maltophilia isolated more from blood samples follow by CSF sample. In addition, our finding illustrated that Stenotrophomonas maltophilia can be considered as the common nosocomial agent at hospitals in Shiraz, Iran.

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