Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Author(s):  
A. Khan ◽  
R. Singh ◽  
S. Sharma ◽  
V. Singh ◽  
A. Sheoran ◽  
...  
2007 ◽  
Vol 55 (2) ◽  
pp. 267-276 ◽  
Author(s):  
L. Sámi ◽  
Krisztina Ursu ◽  
J. McKillen ◽  
S. Kecskeméti ◽  
S. Belák ◽  
...  

Specific oligonucleotide primers were selected and combined in a multiplex arrangement, in order to detect simultaneously three economically important porcine viruses by polymerase chain reaction (PCR). The pathogen panel was comprised of viruses that cause reproductive failure in infected herds: Aujeszky’s disease virus (ADV), porcine parvovirus (PPV) and porcine respiratory and reproductive syndrome virus (PRRSV). In order to reduce the time required for the detection of the pathogens, the assay was optimised to a RapidCycler PCR instrument. The multiplex PCR assay was shown to be specific, sensitive and rapid, because the results were read in less than 60 min after sample preparation. Due to its speed, efficiency and sensitivity, the described rapid multiplex PCR assay serves as a useful novel tool in the veterinary diagnostic laboratories for the quick and complex detection of these important porcine pathogens.


2016 ◽  
Vol 306 (4) ◽  
pp. 212-221 ◽  
Author(s):  
Anna S. Świerzko ◽  
Marcin A. Bartłomiejczyk ◽  
Anna Brzostek ◽  
Jolanta Łukasiewicz ◽  
Mateusz Michalski ◽  
...  

1992 ◽  
Vol 60 (6) ◽  
pp. 2522-2528 ◽  
Author(s):  
H P Godfrey ◽  
Z Feng ◽  
S Mandy ◽  
K Mandy ◽  
K Huygen ◽  
...  

2012 ◽  
Vol 288 (6) ◽  
pp. 3886-3896 ◽  
Author(s):  
Chih-Jung Kuo ◽  
Christopher P. Ptak ◽  
Ching-Lin Hsieh ◽  
Bruce L. Akey ◽  
Yung-Fu Chang

1990 ◽  
Vol 58 (1) ◽  
pp. 272-274 ◽  
Author(s):  
H G Wiker ◽  
K Sletten ◽  
S Nagai ◽  
M Harboe

2007 ◽  
Vol 177 (4S) ◽  
pp. 360-360
Author(s):  
Ana Agud ◽  
Maria J. Ribal ◽  
Lourdes Mengual ◽  
Mercedes Marin-Aguilera ◽  
Laura Izquierdo ◽  
...  

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