scholarly journals An Agrobacterium ‐mediated stable transformation technique for the hornwort model Anthoceros agrestis

2021 ◽  
Author(s):  
Eftychios Frangedakis ◽  
Manuel Waller ◽  
Tomoaki Nishiyama ◽  
Hirokazu Tsukaya ◽  
Xia Xu ◽  
...  
Keyword(s):  
Stable Transformation ◽  
Transformation Technique

scholarly journals A simple Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis

2021 ◽  
Author(s):  
Eftychios Frangedakis ◽  
Manuel Waller ◽  
Tomoaki Nishiyama ◽  
Hirokazu Tsukaya ◽  
Xia Xu ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Genetic Manipulation ◽  
Fluorescent Proteins ◽  
Land Plant ◽  
Plant Evolution ◽  
Stable Transformation ◽  
35S Promoter ◽  
Low Light ◽  
Transformation Technique ◽  
High Transformation

We have developed a simple Agrobacterium-mediated method for the stable transformation of the hornwort Anthoceros agrestis, the fifth bryophyte species for which a genetic manipulation technique becomes available. High transformation efficiency was achieved by using thallus tissue grown under low-light conditions. We generated a total of 216 transgenic A. agrestis lines expressing the β-Glucuronidase (GUS), cyan, green, and yellow fluorescent proteins under the control of the CaMV 35S promoter and several endogenous promoters. Nuclear and plasma membrane localization with multiple color fluorescent proteins was also confirmed. The transformation technique described here should pave the way for detailed molecular and genetic studies of hornwort biology, providing much needed insight into the molecular mechanisms underlying symbiosis, carbon-concentrating mechanism, RNA editing, and land plant evolution in general.

Development of New Image Registration Techniques – A Research Framework

2018 ◽  
Vol 8 (3) ◽  
pp. 50
Author(s):  
Sindhu Madhuri G. ◽  
Indira Gandhi M P
Keyword(s):  
Image Processing ◽  
Image Registration ◽  
Digital Image Processing ◽  
Digital Image ◽  
Creative Thinking ◽  
Reference Image ◽  
Transformation Technique ◽  
Research Framework ◽  
Registration Process ◽  
Data Source

Image is a basic and fundamental data source for the digital image processing. This image data source is required to be processed into information or intelligence and further to knowledge levels where it is required to understand and migrate into knowledge economy systems. Image registration is one of such key and most important process already identified in the digital image processing domain. Image registration is a process of bringing the reference image and sensed image into a common co-ordinate system, and application of complex transformation techniques for necessary comparison of reference with sensed images obtained from different - views, times, spaces, etc., in order to extract the valuable information and intelligence embedded in them. Due to the complexity of overall image registration process, it is difficult to suggest a single transformation technique even for a specific application. In addition, it is highly impossible to suggest one single transformation technique for comparison of various sensed images with a reference image during the image registration process. This research gap calls for the development of new image registration techniques for the application of more than one transformation technique during the image registration process for the necessary comparisons with reference image & sensed images, those are obtained from the available heterogeneous sources or sensors, based on the requirement. In addition, it is a basic need to attempt for the measurement of effectiveness of the image registration process also. Therefore, a research framework is developed for image registration process and attempted for the measurement of its effectiveness also. This new research area is a novel idea, and is expected to emerge as a provision for the knowledge computations with creative thinking through the embedded intelligence extraction during the complex image registration process.

Conservative solute transport with periodic velocity and sinusoidal source concentration in semi-infinite porous media: An analytical solution

2014 ◽  
Vol 6 (1) ◽  
pp. 1024-1031
Author(s):  
R R Yadav ◽  
Gulrana Gulrana ◽  
Dilip Kumar Jaiswal
Keyword(s):  
Porous Medium ◽  
Porous Media ◽  
Laplace Transformation ◽  
Seepage Velocity ◽  
Transformation Technique ◽  
Numerical Examples ◽  
One Dimensional ◽  
Porous Domain ◽  
Conservative Solute Transport ◽  
Source Concentration

The present paper has been focused mainly towards understanding of the various parameters affecting the transport of conservative solutes in horizontally semi-infinite porous media. A model is presented for simulating one-dimensional transport of solute considering the porous medium to be homogeneous, isotropic and adsorbing nature under the influence of periodic seepage velocity. Initially the porous domain is not solute free. The solute is initially introduced from a sinusoidal point source. The transport equation is solved analytically by using Laplace Transformation Technique. Alternate as an illustration; solutions for the present problem are illustrated by numerical examples and graphs.

New Exact Solutions of Steady Plane Flows of an In Compressible Fluid of Variable Viscosity

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Sobia Younus
Keyword(s):  
Exact Solutions ◽  
Stream Function ◽  
Compressible Fluid ◽  
Variable Viscosity ◽  
Plane Motion ◽  
Transformation Technique ◽  
Vorticity Distribution ◽  
New Exact Solutions ◽  
Steady Plane ◽  
Uniform Stream

<span>Some new exact solutions to the equations governing the steady plane motion of an in compressible<span> fluid of variable viscosity for the chosen form of the vorticity distribution are determined by using<span> transformation technique. In this case the vorticity distribution is proportional to the stream function<span> perturbed by the product of a uniform stream and an exponential stream<br /><br class="Apple-interchange-newline" /></span></span></span></span>

scholarly journals New oscillation criteria for second-order neutral differential equations with distributed deviating arguments

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
Osama Moaaz ◽  
Choonkil Park ◽  
Elmetwally M. Elabbasy ◽  
Waed Muhsin
Keyword(s):  
Differential Equations ◽  
Second Order ◽  
The Other ◽  
Riccati Transformation ◽  
Transformation Technique ◽  
Deviating Arguments ◽  
Neutral Differential Equations ◽  
Second Order Differential Equations ◽  
Continuous Delay ◽  
New Criteria

AbstractIn this work, we create new oscillation conditions for solutions of second-order differential equations with continuous delay. The new criteria were created based on Riccati transformation technique and comparison principles. Furthermore, we obtain iterative criteria that can be applied even when the other criteria fail. The results obtained in this paper improve and extend the relevant previous results as illustrated by examples.

Virulence of “white-gray-opaque” tri-stable transformation in clinical Candida albicans in vitro and in vivo

2021 ◽  
Vol 154 ◽  
pp. 104825
Author(s):  
Jing Yang ◽  
Wenli Feng ◽  
Zhiqin Xi ◽  
Lu Yang ◽  
Xiaoxia Zhao ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Stable Transformation
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