ZNF582 Hypermethylation as a Prognostic Biomarker for Malignant Transformation of Oral Lesions

Oral Diseases ◽  
2021 ◽  
Author(s):  
Yi‐Chen Juan ◽  
Yee‐Fun Su ◽  
Chyi‐Huey Bai ◽  
Yen‐Chun Fan ◽  
Tzu‐Tung Kuo ◽  
...  
Keyword(s):  
Malignant Transformation ◽  
Prognostic Biomarker ◽  
Oral Lesions

scholarly journals Incorporation of differentiated dysplasia improves prediction of oral leukoplakia at increased risk of malignant progression

2020 ◽  
Vol 33 (6) ◽  
pp. 1033-1040 ◽  
Author(s):  
Leon J. Wils ◽  
Jos B. Poell ◽  
Ilkay Evren ◽  
Marit S. Koopman ◽  
Elisabeth R. E. A. Brouns ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
World Health Organization ◽  
Cell Carcinoma ◽  
Malignant Transformation ◽  
Squamous Cell ◽  
Malignant Progression ◽  
Oral Leukoplakia ◽  
World Health ◽  
Oral Lesions ◽  
Health Organization

AbstractOral leukoplakia is the most common oral potentially malignant disorder with a malignant transformation rate into oral squamous cell carcinoma of 1–3% annually. The presence and grade of World Health Organization defined dysplasia is an important histological marker to assess the risk for malignant transformation, but is not sufficiently accurate to personalize treatment and surveillance. Differentiated dysplasia, known from differentiated vulvar intraepithelial neoplasia, is hitherto not used in oral dysplasia grading. We hypothesized that assessing differentiated dysplasia besides World Health Organization defined (classic) dysplasia will improve risk assessment of malignant transformation of oral leukoplakia. We investigated a retrospective cohort consisting of 84 oral leukoplakia patients. Biopsies were assessed for dysplasia presence and grade, and the expression of keratins 13 (CK13) and 17, known to be dysregulated in dysplastic vulvar mucosa. In dysplastic oral lesions, differentiated dysplasia is as common as classic dysplasia. In 25 out of 84 (30%) patients, squamous cell carcinoma of the upper aerodigestive tract developed during follow-up. Considering only classic dysplasia, 11 out of 56 (20%) patients with nondysplastic lesions progressed. With the incorporation of differentiated dysplasia, only 2 out of 30 (7%) patients with nondysplastic lesions progressed. The risk of progression increased from 3.26 (Hazard ratio, p = 0.002) when only classic dysplasia is considered to 7.43 (Hazard ratio, p = 0.001) when classic and differentiated dysplasia are combined. Loss of CK13, combined with presence of dysplasia, is associated with greater risk of malignant progression (p = 0.006). This study demonstrates that differentiated dysplasia should be recognized as a separate type of dysplasia in the oral mucosa and that its distinction from classic dysplasia is of pathological and clinical significance since it is a strong (co)prognostic histopathological marker for oral malignant transformation. In oral lesions without dysplasia and retained CK13 staining the risk for progression is very low.

CLIC1 Protein: A Candidate Prognostic Biomarker for Malignant-Transformed Hydatidiform Moles

2011 ◽  
Vol 21 (1) ◽  
pp. 153-160 ◽  
Author(s):  
Zhong-Hua Shi ◽  
Chun Zhao ◽  
Hong Wu ◽  
Wei Wang ◽  
Xiao-Mei Liu
Keyword(s):  
Malignant Transformation ◽  
Gel Electrophoresis ◽  
Prognostic Biomarker ◽  
Hydatidiform Mole ◽  
Protein A ◽  
Two Dimensional Gel Electrophoresis ◽  
Channel Protein ◽  
Proteomic Approach ◽  
Intracellular Channel ◽  
Hydatidiform Moles

Objectives:The purpose of this study was to identify prognostic biomarkers indicating malignant transformation of hydatidiform moles (HMs).Methods:Two-dimensional gel electrophoresis-based proteomic approach was used to compare the protein profiles of complete benign moles (3 samples) with those of malignant-transformed moles (3 samples). Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify differentially expressed proteins. Western blot was used to verify the results of 2-dimensional gel electrophoresis, and immunohistology was used to explore the function of these proteins in gestational trophoblastic disease.Results:Eighteen proteins, deregulated in the malignant-transformed group compared with the benign group (ratio ≥2;P< 0.05), were identified. A bioinformatic analysis indicated that most of these 18 proteins were involved in the processes of cell proliferation and cell survival. Among the 18 proteins, chloride intracellular channel protein 1 (CLIC1) was chosen for further study. Our results showed that the levels of CLIC1 expression in choriocarcinoma tissue were higher than in complete HM tissue (P< 0.01). Chloride intracellular channel protein 1 expression was increased in the tissues of malignant-transformed HMs compared with nontransformed HMs (P< 0.01).Conclusion:Our findings suggest that CLIC1 could be a potential new prognostic biomarker for hydatidiform mole that undergoes malignant transformation.

scholarly journals Expression of invadopodia markers can identify oral lesions with a high risk of malignant transformation

2020 ◽  
Vol 7 (1) ◽  
pp. 61-74
Author(s):  
Aiman Ali ◽  
Andresa Borges Soares ◽  
Denise Eymael ◽  
Marco Magalhaes
Keyword(s):  
High Risk ◽  
Malignant Transformation ◽  
Oral Lesions

scholarly journals Immunohistochemical Study of Laminin-332 γ2 Chain and MMP-9 in High Risk of Malignant Transformation Oral Lesions and OSCC

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Eline Manhães Reid Silva ◽  
Vanessa Morais Freitas ◽  
Willian Grassi Bautz ◽  
Liliana Aparecida Pimenta de Barros ◽  
Letícia Nogueira da Gama de Souza
Keyword(s):  
High Risk ◽  
Malignant Transformation ◽  
Immunohistochemical Study ◽  
Oral Lesions ◽  
Laminin 332

scholarly journals PCNA and p53 expression in oral leukoplakia with different degrees of keratinization

2006 ◽  
Vol 14 (4) ◽  
pp. 276-280 ◽  
Author(s):  
Melaine de Almeida Lawall ◽  
Marcelo Macedo Crivelini
Keyword(s):  
Expression Pattern ◽  
Malignant Transformation ◽  
Oral Mucosa ◽  
Clinical Aspect ◽  
Oral Leukoplakia ◽  
Oral Lesions ◽  
Immunohistochemical Expression ◽  
P53 Expression ◽  
Epithelial Layers ◽  
Epithelial Keratinization

Leukoplakias are oral lesions that may have many clinical and histological aspects and they are usually associated with malignancy when dysplastic alterations are shown. However, these transformations may occur in non-dysplastic lesions that show harmless clinical aspect. For this reason, the proposal was to study the p53 and PCNA immunohistochemical expression in non-dysplastic leukoplakias, trying to correlate the results only with the epithelial keratinization degree. For this, 24 leukoplakias degrees I, II and III of Grinspan were used, all of them located in oral mucosa. Most of the leukoplakias showed p53 and PCNA expression in their different keratinization degrees. The p53 marking was confined to the basal and parabasal layers, while the PCNA marking occurred in practically all epithelial layers. The expression pattern of these markers was histologically and statistically similar between the lesions with these keratinization variations. It was evident that non-dysplastic epithelium of leukoplakias showed submicroscopical signs of alterations that lead to malignant transformation, and that the keratinization degree did not correlate to a greater risk of this event.

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