scholarly journals Zinc finger artificial transcription factor-based nearest inactive analogue/nearest active analogue strategy used for the identification of plant genes controlling homologous recombination

2013 ◽  
Vol 11 (9) ◽  
pp. 1069-1079 ◽  
Author(s):  
Qi Jia ◽  
Marcel C. van Verk ◽  
Johan E. Pinas ◽  
Beatrice I. Lindhout ◽  
Paul J.J. Hooykaas ◽  
...  
2013 ◽  
Vol 14 (1) ◽  
pp. 3 ◽  
Author(s):  
Annalisa Onori ◽  
Cinzia Pisani ◽  
Georgios Strimpakos ◽  
Lucia Monaco ◽  
Elisabetta Mattei ◽  
...  

2018 ◽  
Vol 60 (2) ◽  
pp. 393-406
Author(s):  
Niels van Tol ◽  
Gema Flores Andaluz ◽  
Hendrika A C F Leeggangers ◽  
M Reza Roushan ◽  
Paul J J Hooykaas ◽  
...  

2006 ◽  
Vol 4 (3) ◽  
pp. 317-331 ◽  
Author(s):  
Adriana Beltran ◽  
Yanzheng Liu ◽  
Shaunak Parikh ◽  
Brenda Temple ◽  
Pilar Blancafort

2018 ◽  
Vol 31 (Supplement_1) ◽  
pp. 133-134
Author(s):  
Tomoki Yamatsuji ◽  
Etsuko Yokota ◽  
Takashi Sera ◽  
Noriaki Manabe ◽  
Takuya Fukazawa ◽  
...  

Abstract Background SOX2 is a transcription factor that is fundamental for early development and for the maintenance of stem cells in multiple adult tissues and also plays an important role in squamous cell differentiation. Amplification of chromosome 3q26 is the most common of the genetic alterations found in squamous cell carcinoma (SCC). SOX2 is a candidate oncogene present in this locus and amplification of SOX2 has been reported in lung and esophageal squamous cell SCC. In this study, we have developed a zinc finger-based artificial transcription factor (ATF) to selectively suppress SOX2 expression in cancer cells and termed the system ATF/SOX2. Methods We engineered the ATF using six zinc finger arrays designed to target a 19 bp site in the SOX2 distal promoter and a KOX transcriptional repressor domain. A recombinant adenoviral vector Ad-ATF/SOX2 that expresses ATF/SOX2 suppressed SOX2 at the mRNA and protein levels in esophageal SCC cells(TE1 and TE4) expressing SOX2. Results Ad-ATF/SOX2 decreased esophageal SCC cells proliferation and colony formation more effectively than the recombinant adenoviral vector Ad-shSOX2, which expresses SOX2 short hairpin RNA (shSOX2). Ad-ATF/SOX2 induced the cell cycle inhibitor CDKN1A more strongly than Ad-shSOX2. Moreover, Ad-ATF/SOX2 effectively inhibited tumor growth in a SCC xenograft mouse model. Conclusion In this study, we have shown that the targeted down-regulation of SOX2 using ATF based technologies can be used as an effective tool for the treatment of SCC in esophageal cancers that express SOX2. Our results indicate that ATF/SOX2 would lead to the development of an effective molecular-targeted therapy for esophageal SCC. Disclosure All authors have declared no conflicts of interest.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Kuo Yang ◽  
Jian-Ping An ◽  
Chong-Yang Li ◽  
Xue-Na Shen ◽  
Ya-Jing Liu ◽  
...  

AbstractJasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.


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