Molecular loci associated with seed isoflavone content may underlie resistance to soybean pod borer (Leguminivora glycinivorella)

2015 ◽  
Vol 134 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Guiyun Zhao ◽  
Zhenfeng Jiang ◽  
Dongmei Li ◽  
Yingpeng Han ◽  
Haibo Hu ◽  
...  
Keyword(s):  
Pod Borer ◽  
Isoflavone Content ◽  
Seed Isoflavone

scholarly journals Genetic control of soybean seed isoflavone content: importance of statistical model and epistasis in complex traits

2009 ◽  
Vol 119 (6) ◽  
pp. 1069-1083 ◽  
Author(s):  
Juan Jose Gutierrez-Gonzalez ◽  
Xiaolei Wu ◽  
Juan Zhang ◽  
Jeong-Dong Lee ◽  
Mark Ellersieck ◽  
...  
Keyword(s):  
Statistical Model ◽  
Genetic Control ◽  
Complex Traits ◽  
Soybean Seed ◽  
Isoflavone Content ◽  
Seed Isoflavone

scholarly journals Genomic Regions That Underlie Soybean Seed Isoflavone Content

2001 ◽  
Vol 1 (1) ◽  
pp. 38-44 ◽  
Author(s):  
K. Meksem ◽  
V. N. Njiti ◽  
W. J. Banz ◽  
M. J. Iqbal ◽  
My. M. Kassem ◽  
...  
Keyword(s):  
Linkage Group ◽  
High Performance ◽  
Biological Effects ◽  
Recombinant Inbred Lines ◽  
Soybean Seed ◽  
Soybean Seeds ◽  
Isoflavone Content ◽  
Seed Isoflavone ◽  
Genomic Regions

Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of ‘Essex’ by ‘Forrest,’ two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P=0.0001,R 2=50.2%), linkage groupNcontained a QTL for glycitein (P=0.0033,R 2=11.1%) and a QTL for daidzein (P=0.0023,R 2=10.3%) and linkage groupA1contained a QTL for daidzein (P=0.0081,R 2=9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

scholarly journals Effect of different planting times on the quantitative variation of total seed isoflavone content and composition in Korean soybean cultivars (Glycine max (L.) Merr.)

2020 ◽  
Author(s):  
Hong-Sik Kim ◽  
Beom-Kyu Kang ◽  
Jeong-Hyun Seo ◽  
Hyun-Tae Kim ◽  
Tae-Joung Ha ◽  
...  
Keyword(s):  
Ecological Environment ◽  
Average Content ◽  
Functional Ingredients ◽  
Black Soybean ◽  
Planting Time ◽  
Soybean Cultivars ◽  
Isoflavone Content ◽  
Glycine Max L ◽  
Seed Isoflavone ◽  
Total Seed

Abstract There is great interest in the enhancement of isoflavones as one of the functional ingredients in soybean. This study aimed to investigate the effects of changes in the ecological environment over different planting times on isoflavone content. A total of 28 Korean soybean cultivars were grown at different planting times in late May, mid-June, and early July and their isoflavone content was measured over 2 years (2017 and 2018). Analyses of variance revealed significant effects of genotypes, planting times, years, and their interactions on isoflavone content. The average content of total isoflavone, as well as its component groups of malonylglucoside and aglycon, increased significantly as the seed planting time was delayed from late May to early July. The accumulation of each isoflavone component varied with changes in the planting time. The isoflavone content of the soybean cultivars for soy-sprout and soy-paste and tofu were higher for plantings in early July than for those in late May and/or mid-June, except for the black soybean cultivars. Despite significant correlations of the isoflavone content of the 28 cultivars among the three planting times, the responses of individual cultivars varied in isoflavone content by planting time. When planting was delayed, the time to flowering and maturity was also delayed and the number of days of growth from planting or flowering to maturity decreased; however, this was not related to isoflavone content. When planting was delayed, the temperature during the ripening period from flowering to maturity was lower, which was inversely related to the isoflavone content.

scholarly journals Definition of Soybean Genomic Regions That Control Seed Phytoestrogen Amounts

2004 ◽  
Vol 2004 (1) ◽  
pp. 52-60 ◽  
Author(s):  
My A. Kassem ◽  
K. Meksem ◽  
M. J. Iqbal ◽  
V. N. Njiti ◽  
W. J. Banz ◽  
...  
Keyword(s):  
Linkage Group ◽  
Dna Markers ◽  
Biological Effects ◽  
Heritable Variation ◽  
Control Seed ◽  
Isoflavone Content ◽  
Seed Isoflavone ◽  
Definition Of ◽  
Genomic Regions ◽  
Beneficial Allele

Soybean seeds contain large amounts of isoflavones or phytoestrogens such as genistein, daidzein, and glycitein that display biological effects when ingested by humans and animals. In seeds, the total amount, and amount of each type, of isoflavone varies by 5 fold between cultivars and locations. Isoflavone content and quality are one key to the biological effects of soy foods, dietary supplements, and nutraceuticals. Previously we had identified 6 loci (QTL) controlling isoflavone content using 150 DNA markers. This study aimed to identify and delimit loci underlying heritable variation in isoflavone content with additional DNA markers. We used a recombinant inbred line (RIL) population (n=100) derived from the cross of “Essex” by “Forrest,” two cultivars that contrast for isoflavone content. Seed isoflavone content of each RIL was determined by HPLC and compared against 240 polymorphic microsatellite markers by one-way analysis of variance. Two QTL that underlie seed isoflavone content were newly discovered. The additional markers confirmed and refined the positions of the six QTL already reported. The first new region anchored by the marker BARC-Satt063 was significantly associated with genistein (P=0.009,R2=29.5%) and daidzein (P=0.007,R2=17.0%). The region is located on linkage group B2 and derived the beneficial allele from Essex. The second new region defined by the marker BARC-Satt129 was significantly associated with total glycitein (P=0.0005,R2=32.0%). The region is located on linkage group D1a+Q and also derived the beneficial allele from Essex. Jointly the eight loci can explain the heritable variation in isoflavone content. The loci may be used to stabilize seed isoflavone content by selection and to isolate the underlying genes.

scholarly journals EDAMAME VEGETABLE SOYBEAN PRODUCTION IN TENNESSEE

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 504B-504
Author(s):  
Debra Carpenter ◽  
Vince Pantalone ◽  
Fred Allen ◽  
Dennis Deyton ◽  
Carl Sams ◽  
...  
Keyword(s):  
Cropping System ◽  
Plant Spacing ◽  
Planting Dates ◽  
Yield Data ◽  
Double Cropping ◽  
Isoflavone Content ◽  
East Tennessee ◽  
Middle Tennessee ◽  
Seed Isoflavone ◽  
Black Plastic

There are three objectives for this study: to determine the within-row plant spacing and time of planting that will produce optimal yields and seed isoflavone content, to explore the feasibility of incorporating edamame soybeans in a double-cropping system with strawberries, and to study the potential as an edamame soybean of newly identified line TN03-349. TN03-349 was planted into black plastic, irrigated strawberry beds in an East Tennessee location at five different within-row spacings (7.62, 15.24, 30.48, 60.96, and 121.92 cm) in 2004 and 2005. Another strawberry bed planting was located in Middle Tennessee in 2005. Four soybean lines and two planting dates were used in the Middle Tennessee experiment. Two lines are high yielding soybean checks, while the third is a commercially available edamame cultivar. The fourth line is TN03-349. Planting dates were 24 May and 14 June 2005. A final field experiment utilized the same four soybean lines and planting dates with an additional planting on 6 July 2005. Four different within-row spacings were used, as well. All experimental plantings were harvested at both the R6 (green) and R8 (dry) stages. Preliminary data indicates that isoflavone content was not affected by within-row spacing in the 2004 East Tennessee strawberry bed experiment. Yield data from the same experiment seems to indicate that soybeans were able to compensate for fewer plants per row at the 7, 62, 15.24, and 30.48 cm spacings. Yield dropped sharply at the 60.96 and 121.92 cm treatments. Line TN03-349 produced beans with large seed size and nutty flavor, traits that are essential for edamame soybeans.

scholarly journals Additional Quantitative Trait Loci and Candidate Genes for Seed Isoflavone Content in Soybean

2013 ◽  
Vol 5 (11) ◽  
Author(s):  
M. Akond ◽  
Bazelle Richard ◽  
Bobby Ragin ◽  
Harmin Herrera ◽  
Umerah Kaodi ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Isoflavone Content ◽  
Trait Loci ◽  
Seed Isoflavone

scholarly journals The Soybean High Density ‘Forrest’ by ‘Williams 82’ SNP-Based Genetic Linkage Map Identifies QTL and Candidate Genes for Seed Isoflavone Content

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2029
Author(s):  
Dounya Knizia ◽  
Jiazheng Yuan ◽  
Nacer Bellaloui ◽  
Tri Vuong ◽  
Mariola Usovsky ◽  
...  
Keyword(s):  
Linkage Map ◽  
Candidate Genes ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Association Studies ◽  
Genome Wide Association Studies ◽  
Health And Nutrition ◽  
Legume Seeds ◽  
Isoflavone Content ◽  
Seed Isoflavone

Isoflavones are secondary metabolites that are abundant in soybean and other legume seeds providing health and nutrition benefits for both humans and animals. The objectives of this study were to construct a single nucleotide polymorphism (SNP)-based genetic linkage map using the ‘Forrest’ by ‘Williams 82’ (F×W82) recombinant inbred line (RIL) population (n = 306); map quantitative trait loci (QTL) for seed daidzein, genistein, glycitein, and total isoflavone contents in two environments over two years (NC-2018 and IL-2020); identify candidate genes for seed isoflavone. The FXW82 SNP-based map was composed of 2075 SNPs and covered 4029.9 cM. A total of 27 QTL that control various seed isoflavone traits have been identified and mapped on chromosomes (Chrs.) 2, 4, 5, 6, 10, 12, 15, 19, and 20 in both NC-2018 (13 QTL) and IL-2020 (14 QTL). The six QTL regions on Chrs. 2, 4, 5, 12, 15, and 19 are novel regions while the other 21 QTL have been identified by other studies using different biparental mapping populations or genome-wide association studies (GWAS). A total of 130 candidate genes involved in isoflavone biosynthetic pathways have been identified on all 20 Chrs. and among them 16 have been identified and located within or close to the QTL identified in this study. Moreover, transcripts from four genes (Glyma.10G058200, Glyma.06G143000, Glyma.06G137100, and Glyma.06G137300) were highly abundant in Forrest and Williams 82 seeds. The identified QTL and four candidate genes will be useful in breeding programs to develop soybean cultivars with high beneficial isoflavone contents.

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