scholarly journals Genomic Regions That Underlie Soybean Seed Isoflavone Content

2001 ◽  
Vol 1 (1) ◽  
pp. 38-44 ◽  
Author(s):  
K. Meksem ◽  
V. N. Njiti ◽  
W. J. Banz ◽  
M. J. Iqbal ◽  
My. M. Kassem ◽  
...  
Keyword(s):  
Linkage Group ◽  
High Performance ◽  
Biological Effects ◽  
Recombinant Inbred Lines ◽  
Soybean Seed ◽  
Soybean Seeds ◽  
Isoflavone Content ◽  
Seed Isoflavone ◽  
Genomic Regions

Soy products contain isoflavones (genistein, daidzein, and glycitein) that display biological effects when ingested by humans and animals, these effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key to their biological effect. Our objective was to identify loci that underlie isoflavone content in soybean seeds. The study involved 100 recombinant inbred lines (RIL) from the cross of ‘Essex’ by ‘Forrest,’ two cultivars that contrast for isoflavone content. Isoflavone content of seeds from each RIL was determined by high performance liquid chromatography (HPLC). The distribution of isoflavone content was continuous and unimodal. The heritability estimates on a line mean basis were 79% for daidzein, 22% for genistein, and 88% for glycitein. Isoflavone content of soybean seeds was compared against 150 polymorphic DNA markers in a one-way analysis of variance. Four genomic regions were found to be significantly associated with the isoflavone content of soybean seeds across both locations and years. Molecular linkage group B1 contained a major QTL underlying glycitein content (P=0.0001,R 2=50.2%), linkage groupNcontained a QTL for glycitein (P=0.0033,R 2=11.1%) and a QTL for daidzein (P=0.0023,R 2=10.3%) and linkage groupA1contained a QTL for daidzein (P=0.0081,R 2=9.6%). Selection for these chromosomal regions in a marker assisted selection program will allow for the manipulation of amounts and profiles of isoflavones (genistein, daidzein, and glycitein) content of soybean seeds. In addition, tightly linked markers can be used in map based cloning of genes associated with isoflavone content.

scholarly journals Definition of Soybean Genomic Regions That Control Seed Phytoestrogen Amounts

2004 ◽  
Vol 2004 (1) ◽  
pp. 52-60 ◽  
Author(s):  
My A. Kassem ◽  
K. Meksem ◽  
M. J. Iqbal ◽  
V. N. Njiti ◽  
W. J. Banz ◽  
...  
Keyword(s):  
Linkage Group ◽  
Dna Markers ◽  
Biological Effects ◽  
Heritable Variation ◽  
Control Seed ◽  
Isoflavone Content ◽  
Seed Isoflavone ◽  
Definition Of ◽  
Genomic Regions ◽  
Beneficial Allele

Soybean seeds contain large amounts of isoflavones or phytoestrogens such as genistein, daidzein, and glycitein that display biological effects when ingested by humans and animals. In seeds, the total amount, and amount of each type, of isoflavone varies by 5 fold between cultivars and locations. Isoflavone content and quality are one key to the biological effects of soy foods, dietary supplements, and nutraceuticals. Previously we had identified 6 loci (QTL) controlling isoflavone content using 150 DNA markers. This study aimed to identify and delimit loci underlying heritable variation in isoflavone content with additional DNA markers. We used a recombinant inbred line (RIL) population (n=100) derived from the cross of “Essex” by “Forrest,” two cultivars that contrast for isoflavone content. Seed isoflavone content of each RIL was determined by HPLC and compared against 240 polymorphic microsatellite markers by one-way analysis of variance. Two QTL that underlie seed isoflavone content were newly discovered. The additional markers confirmed and refined the positions of the six QTL already reported. The first new region anchored by the marker BARC-Satt063 was significantly associated with genistein (P=0.009,R2=29.5%) and daidzein (P=0.007,R2=17.0%). The region is located on linkage group B2 and derived the beneficial allele from Essex. The second new region defined by the marker BARC-Satt129 was significantly associated with total glycitein (P=0.0005,R2=32.0%). The region is located on linkage group D1a+Q and also derived the beneficial allele from Essex. Jointly the eight loci can explain the heritable variation in isoflavone content. The loci may be used to stabilize seed isoflavone content by selection and to isolate the underlying genes.

scholarly journals Genetic control of soybean seed isoflavone content: importance of statistical model and epistasis in complex traits

2009 ◽  
Vol 119 (6) ◽  
pp. 1069-1083 ◽  
Author(s):  
Juan Jose Gutierrez-Gonzalez ◽  
Xiaolei Wu ◽  
Juan Zhang ◽  
Jeong-Dong Lee ◽  
Mark Ellersieck ◽  
...  
Keyword(s):  
Statistical Model ◽  
Genetic Control ◽  
Complex Traits ◽  
Soybean Seed ◽  
Isoflavone Content ◽  
Seed Isoflavone

scholarly journals Application of Bioactive Coatings Based on Chitosan for Soybean Seed Protection

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Defang Zeng ◽  
Xinrong Luo ◽  
Renjie Tu
Keyword(s):  
Soybean Seed ◽  
Soybean Aphid ◽  
Soybean Seeds ◽  
Bioactive Coatings ◽  
Coating Agent ◽  
Soybean Yield ◽  
Chitosan Coating ◽  
Pod Borer ◽  
Made In

Soybean seeds suffer attacks of various pests that result in a decreased yield in northeastern China. Until recently, people use pesticides such as insecticides to achieve the goal of controlling pests. Chitosan extracted from deacetylation of chitin is promising candidates as a seed-coating agent to control agrotis ypsilon, soybean pod borer, and soybean aphid effectively. An experimental study on influences of chitosan with different concentrations on pest controlling and soybean growth was made in the paper. Coating based on chitosan was used as a feeding deterrent and for enhancing the germination and quality of soybean seeds. Results indicated that all chitosan coating had a significant effect on antifeeding against pests; with the increasing concentration, antifeedant rate (AR) were increased obviously, especially when in the concentration of 5%, santifeedant rate of agrotis ypsilon, soybean pod borer, and soybean aphid reached 82.89%, 87.24%, and 80.21%, respectively. Also chitosan coating increased seed germination, plant growth, and soybean yield efficiently, especially when, in the concentration of 5%, the yield was increased by about 20% compared with CK. The application of chitosan in soybean seed coated is an appropriate option to control pests replacing high-toxicity pesticides and enhance soybean yield.

scholarly journals TECHNOLOGICAL PROCESSES OF SOY DRYING

2019 ◽  
pp. 52-58
Author(s):  
Valentyna Bandura ◽  
Oleksandr Popiak
Keyword(s):  
Electromagnetic Field ◽  
Comparative Analysis ◽  
Technological Process ◽  
State Standard ◽  
Soybean Seed ◽  
The State ◽  
Drying Methods ◽  
Soybean Seeds ◽  
Technological Processes

Different technologies of soybean seed drying are considered, their positive and negative sides are revealed. A comparative analysis of drying methods was carried out. It is established that the drying of soybean seeds in an electromagnetic field is one of the most promising. A combination of two methods of drying infrared and microwave soybean seeds in a single technological process is proposed. The requirements for the quality of soybean seeds are considered when stored in accordance with the State Standard, in particular the observance of humidity, which should not be more than 12%.

scholarly journals EFFECT OF Fusarium graminearum Schwabe ON PHYSIOLOGICAL QUALITY OF SOYBEAN SEEDS AND WHEAT CARYOPSIS IN ARGENTINA

2015 ◽  
Vol 28 (3) ◽  
pp. 1-11
Author(s):  
ALEJANDRA MARÍA PERUZZO ◽  
ROSANNA NORA PIOLI ◽  
ADRIANA RITA SALINAS
Keyword(s):  
Seed Quality ◽  
Natural Infection ◽  
Soybean Seed ◽  
Diagnostic Techniques ◽  
Soybean Seeds ◽  
Santa Fe ◽  
Head Blight ◽  
Physiological Quality ◽  
Artificial Inoculations

ABSTRACT: F. graminearum is the main causal agent of Head blight in cereals in Argentina. This is a disease that develops during the host floral state. When the reproductive structures in the host are invaded, grains may be shriveled and reduced in weight, causing a decrease in yield. Physiological diagnostic techniques on seeds detect the damages produced by this fungus could be used to take decisions related to the quality of seed lots. The objective of this study was to evaluate the possible physiological damage caused by F. graminearum isolates in soybean seeds and wheat caryopsis. Seeds and caryopsis were obtained from plants exposed to fungal infection and were evaluated under two situations: artificial inoculations under greenhouse conditions and natural infection from fields of Santa Fe Province (33°43'22''S; 62°14'46''W). Seed weight, topographical tetrazolium test, standard germination test, electrical conductivity test and X-ray test were performed in soybean seeds and wheat caryopsis from each treatment. Differential behaviors of F. graminearum strains in susceptible soybean and wheat cultivars under greenhouse conditions revealed specific interactions among soybean and wheat genotypes with this fungus. F. graminearum infection in susceptible cultivars under greenhouse conditions produced a significant decrease in the physiological quality of soybean seed and wheat caryopsis. These behaviors were not detected under field conditions in the evaluated locations. All seed quality tests used in this experiment were useful to show differences in infection in soybean and wheat independently of F. graminearum infection.

scholarly journals Biostimulants and the antiradical activity of soybean seeds

2018 ◽  
Vol 10 ◽  
pp. 01008
Author(s):  
Anna Kocira
Keyword(s):  
Growth And Development ◽  
Antiradical Activity ◽  
Foliar Application ◽  
Soybean Seed ◽  
Growing Season ◽  
Soybean Seeds ◽  
Abts Assay ◽  
The Third ◽  
Yield And Quality

In recent years, the interest in the application of biostimulants has increased, which positively affect the growth and development of plants and contribute to an increase in the yield and quality of crops. In Poland, the area of soybean cultivation is constantly increasing and thus the improvement of the quality of its seeds seems to be purposeful. In the available literature, there are few reports regarding the effect of biostimulants on the antiradical activity of plants. Therefore, studies on the influence of biostimulants on soybean seed antiradical activity seem to be justified. The study was carried out in 2014 - 2016 in Perespa, Poland. Annushka soybean seeds were sown in the third decade of April. During the growing season, four biostimulants: Kelpak SL, Terra Sorb Complex, Atonik, and Tytanit, were used in four combinations, using lower or higher concentrations and single or double spraying. After harvesting the plants, the antiradical activity of the seeds was evaluated by ABTS•+ assay. It has been found that the foliar application of biostimulants positively influenced the studied property. The highest antiradical activity of plants was found upon double spraying with lower concentrations of Atonik and Terra Sorb Complex.

scholarly journals Effect of Sowing Date on Germination and Vigour of Soybean (Glycine max (L.) Merr) Seeds

2013 ◽  
Vol 11 (1) ◽  
pp. 67-75 ◽  
Author(s):  
MM Rahman ◽  
MM Rahman ◽  
MM Hossain
Keyword(s):  
Soybean Seed ◽  
Sowing Date ◽  
Soybean Seeds ◽  
Rabi Season ◽  
Sowing Dates ◽  
High Germination ◽  
Physiological Quality ◽  
Glycine Max L ◽  
Plot Design

An experiment was carried out at the Agronomy Field Laboratory of Bangladesh Agricultural University, Mymensingh during three consecutive seasons in 2004-2006 to find out the effect of sowing date on physiological quality of soybean seed. The experiments included six sowing dates viz. 4 Nov, 24 Nov, 14 Dec, 3 Jan, 23 Jan and 12 Feb and two cultivars namely G-2 and PB-1 in Rabi 2004-05; four sowing dates viz. 25 Jul, 14 Aug, 3 Sept and 23 Sept and three cultivars namely G-2, PB-1 and BS-5 in Kharif II 2005; four sowing dates viz. 26 Nov, 16 Dec, 5 Jan, and 25 Jan, and two cultivars viz. G-2 and PB-1 in Rabi 2005-06. The experiments were conducted in split-plot design with three replications. Results revealed that cultivar G-2 had higher germination and vigour than cultivar PB-1 or BS-5 in all the three seasons. November and December sowing produced seeds with higher germination and vigour for all the cultivars in Rabi season. On the other hand, September sowing produced seeds with high germination and vigour during Kharif II. The study indicated that soybean seeds with high germination and vigour could be obtained by sowing during November to December in Rabi season and August to September in Kharif-II season. DOI: http://dx.doi.org/10.3329/agric.v11i1.15245 The Agriculturists 2013; 11(1) 67-75

scholarly journals Efeito do tratamento químico com inseticida/fungicida e polímero na qualidade fisiológica da semente de soja - Effect of chemical treatment with insecticide / fungicide and polymer on the physiological quality of soybean seed

2017 ◽  
Vol 4 (1) ◽  
pp. 12 ◽  
Author(s):  
Evânia Alves ◽  
Elder Aguiar ◽  
Elder Aguiar ◽  
Célio Pereira ◽  
Célio Pereira ◽  
...  
Keyword(s):  
Glycine Max ◽  
Chemical Treatment ◽  
Seed Treatment ◽  
Soybean Seed ◽  
Soybean Seeds ◽  
Germination Test ◽  
M 10 ◽  
De Se ◽  
Physiological Quality

RESUMOA soja é uma das culturas mais cultivadas do planeta, sendo o Brasil, um de seus maiores produtores. É uma fonte barata e rica de óleo vegetal. O tratamento de sementes é amplamente utilizado para evitar perdas por patógenos e insetos na fase inicial do estabelecimento das plantas. Sendo uma das formas de se evitar a perda da qualidade. O objetivo deste trabalho foi avaliar o efeito do tratamento químico com inseticida/fungicida e polímero na qualidade final das sementes de soja. Foram utilizadas sementes do cultivar M-7110 Ipro, produzida na safra 2016/17 na região de Rio Verde, GO, com grau de umidade de 12%. Os tratamentos de sementes utilizados foram: Testemunha, Caixa vigor e praga (200 mL de Cruiser+ 100 mL de fipronil + 100 mL de Maxim XL); 100 mL + 100 mL de Standak + Maxim XL (Fipronil 250 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L) e 300 mL + 100 mL de Cropstar + Maxim XL (Imidacloprido 150 g/L e Tiodicarbe 450 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L). Foram utilizados o teste de germinação, emergência e a taxa de crescimento de plântulas. O tratamento Cropstar + Maxim XL se destacou como o melhor para o tratamento das sementes de soja quando comparado aos outros tratamentos utilizados neste trabalho. O uso de Standak + Maxim ML foi inferior aos demais para o tratamento de sementes de soja.Palavras-Chave: tratamento de sementes; Glycine max; vigor.ABSTRACTSoy is one of the most cultivated crops on the planet, being Brazil, one of its largest producers. It is a cheap, rich vegetable oil fountain. Seed treatment is widely used to avoid losses by pathogens and insects in the early stages of plant establishment. Being one of the ways to avoid the loss of quality. The purpose of this work was to evaluate the effect of chemical treatment with insecticide/fungicide and polymer in the final quality of soybean seeds. Seeds of cultivar M-7110 Ipro, produced in the harvest 2016/17 in the region of Rio Verde, GO, with a moisture content of 12% were used. The seeds treatments used were: Control, Caixa vigor e praga (200 mL de Cruiser+ 100 mL de fipronil + 100 mL de Maxim XL); 100 mL + 100 mL de Standak + Maxim XL (Fipronil 250 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L) e 300 mL + 100 mL de Cropstar + Maxim XL (Imidacloprido 150 g/L e Tiodicarbe 450 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L). The germination test, emergency and the growth rate of seedlings were tested. The treatment Cropstar + Maxim XL stood out as the best for treating soybean seeds when compared to other treatments used in this work. The use of Standak + Maxim ml was inferior to the other for the treatment of soybean seeds.Keywords: seed treatment; Glycine max; vigour

scholarly journals Effect of soybean seed priming on germination energy

2018 ◽  
Vol 63 (4) ◽  
pp. 335-342
Author(s):  
Zlatica Miladinov ◽  
Svetlana Balesevic-Tubic ◽  
Vojin Djukic ◽  
Aleksandar Ilic ◽  
Lazar Cobanovic ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Seed Quality ◽  
Soybean Seed ◽  
Seed Priming ◽  
Soybean Seeds ◽  
Germination Energy ◽  
Seed Age ◽  
One Year ◽  
And Control

Six varieties were chosen from three different maturity groups: Galina and NS Princeza (0), Sava and NS Apolo (I), and Rubin and NS Zita (II) in order to examine the effect of priming on germination energy depending on soybean seed age. The seeds were produced in the period from 2012 to 2014, i.e., one- to three-year-old seeds were used. The following treatments were used for priming: distilled water - hydropriming (H2O), ascorbic acid 250 mg/l (ASA), folic acid 15 mg/l (Fol.), hydrogen peroxide 1% (H2O2), and control - non-treated seeds (C). Results showed that the effects of priming depended on the variety, seed age, as well as on the applied treatment. Some varieties reacted to priming very well, while others had an inhibited reaction, so germination energy was significantly reduced. Rubin variety had the most favourable reaction, whereby all treatments of this variety led to an increase of quality, with an average increase of 2-8%. The application of primers on one-year-old soybean seeds had a weaker effect than on two- and three-year-old seeds. However, one-year-old seeds had minor oscillations in quality due to priming. The application of priming on one-year-old seeds can lead to an increase or decrease of germination energy by 3%, while two- and three-year-old seeds reached 7%. The results showed that seed priming in ascorbic acid had a positive effect, while the application of other primers did not affect the value of germination energy, nor did it lead to a decrease of its value. Ascorbic acid activity led to the largest increase in the quality of three-year-old soybean seeds by 2.83%, while the increase in the quality of two- and three-year-old seeds was 1.87%. Therefore, we can conclude that there is no universal use of one single primer, as it might not be suitable for each particular variety and can ultimately result in the seed quality decrease.

scholarly journals Specific Genomic Regions in Common Bean Condition Resistance to Multiple Pathogens

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 451E-451
Author(s):  
Phillip N. Miklas ◽  
Valerie Stone ◽  
Carlos A. Urrea ◽  
James S. Beaver
Keyword(s):  
Linkage Group ◽  
Common Bean ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Recombinant Inbred Lines ◽  
Macrophomina Phaseolina ◽  
Major Effect ◽  
Common Bacterial Blight ◽  
Stem Blight ◽  
Genomic Regions

A genetic linkage map of 170 RAPD markers mapped across 79 recombinant inbred lines (Dorado and XAN-176) reveal genomic regions that condition multiple disease resistance to fungal (Ashy Stem Blight—Macrophomina phaseolina), viral (bean golden mosaic virus—BGMV), and bacterial (common bacterial blight—Xanthomonas campestris pv. phaseoli) pathogens of common bean (Phaseolus vulgaris). A genomic site on linkage group US-1 had a major effect, explaining 18%, 34%, and 40% of the variation in phenotypic reaction to ashy stem blight, BGMV, and common bacterial blight disease, respectively. Adjacent to this region was a QTL conditioning 23% of the variation in reaction to another fungal pathogen, web blight (Thanatephorus cucumeris). A second genomic site on linkage group US-1 had minor affect on multiple resistance expression to the same fungal (15%), viral (15%), and bacterial (10%) pathogens. It is unknown whether these specific genomic regions represent a series of linked QTL affecting resistance to each disease separately or an individual locus with pleiotropic effect against all three pathogens.

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