The possible role of BnaA10.SOI.a in seed fatty acid biosynthesis of rapeseed

2019 ◽  
Vol 139 (1) ◽  
pp. 167-175
Author(s):  
Jingyun Gong ◽  
Dong Li ◽  
Xinye Li ◽  
Xuchen Yu ◽  
Yuan Guo ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Seed Fatty Acid

The role of β-ketoacyl-acyl carrier protein synthase III in the condensation steps of fatty acid biosynthesis in sunflower

Planta ◽  
2010 ◽  
Vol 231 (6) ◽  
pp. 1277-1289 ◽  
Author(s):  
Damián González-Mellado ◽  
Penny von Wettstein-Knowles ◽  
Rafael Garcés ◽  
Enrique Martínez-Force
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Acid Biosynthesis

scholarly journals Genome-Wide Association Study of Genetic Control of Seed Fatty Acid Biosynthesis in Brassica napus

2017 ◽  
Vol 7 ◽  
Author(s):  
Katarzyna Gacek ◽  
Philipp E. Bayer ◽  
Iwona Bartkowiak-Broda ◽  
Laurencja Szala ◽  
Jan Bocianowski ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Association Study ◽  
Genetic Control ◽  
Genome Wide Association Study ◽  
Fatty Acid Biosynthesis ◽  
Genome Wide Association ◽  
Acid Biosynthesis ◽  
Seed Fatty Acid ◽  
Genome Wide

Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules

2020 ◽  
Vol 71 (22) ◽  
pp. 6969-6987
Author(s):  
Naoufal Lakhssassi ◽  
Zhou Zhou ◽  
Shiming Liu ◽  
Sarbottam Piya ◽  
Mallory A Cullen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Stearic Acid ◽  
Unsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acyl Carrier Protein ◽  
Nodule Development ◽  
Acid Biosynthesis ◽  
High Stearic Acid ◽  
Tilling By Sequencing

Abstract Developing soybean lines with high levels of stearic acid is a primary goal of the soybean industry. Most high-stearic-acid soybeans carry different GmSACPD-C mutated alleles. However, due to the dual role of GmSACPD-C in seeds and nodule development, all derived deleterious GmSACPD-C mutant alleles are of extremely poor agronomic value because of defective nodulation. The soybean stearoyl-acyl carrier protein desaturase (GmSACPD) gene family is composed of five members. Comparative genomics analysis indicated that SACPD genes were duplicated and derived from a common ancestor that is still present in chlorophytic algae. Synteny analysis showed the presence of segment duplications between GmSACPD-A/GmSACPD-B, and GmSACPD-C/GmSACPD-D. GmSACPD-E was not contained in any duplicated segment and may be the result of tandem duplication. We developed a TILLING by Target Capture Sequencing (Tilling-by-Sequencing+) technology, a versatile extension of the conventional TILLING by sequencing, and successfully identified 12, 14, and 18 ethyl methanesulfonate mutants at the GmSACPD-A, GmSACPD-B, and GmSACPD-D genes, respectively. Functional analysis of all identified mutants revealed an unprecedented role of GmSACPD-A, GmSACPD-B, and GmSACPD-D in unsaturated fatty acid biosynthesis without affecting nodule development and structure. This discovery will positively impact the development of high-stearic-acid lines to enhance soybean nutritional value without potential developmental tradeoffs.

scholarly journals ‘FAS’t inhibition of malaria

2004 ◽  
Vol 383 (3) ◽  
pp. 401-412 ◽  
Author(s):  
Avadhesha SUROLIA ◽  
T. N. C. RAMYA ◽  
V. RAMYA ◽  
Namita SUROLIA
Keyword(s):  
Fatty Acid ◽  
Drug Targets ◽  
Fatty Acid Biosynthesis ◽  
Present Review ◽  
Acid Biosynthesis ◽  
The Status ◽  
Novel Drug ◽  
Emergence Of Resistance ◽  
Specific Pathway

Malaria, a tropical disease caused by Plasmodium sp., has been haunting mankind for ages. Unsuccessful attempts to develop a vaccine, the emergence of resistance against the existing drugs and the increasing mortality rate all call for immediate strategies to treat it. Intense attempts are underway to develop potent analogues of the current antimalarials, as well as a search for novel drug targets in the parasite. The indispensability of apicoplast (plastid) to the survival of the parasite has attracted a lot of attention in the recent past. The present review describes the origin and the essentiality of this relict organelle to the parasite. We also show that among the apicoplast specific pathways, the fatty acid biosynthesis system is an attractive target, because its inhibition decimates the parasite swiftly unlike the ‘delayed death’ phenotype exhibited by the inhibition of the other apicoplast processes. As the enzymes of the fatty acid biosynthesis system are present as discrete entities, unlike those of the host, they are amenable to inhibition without impairing the operation of the host-specific pathway. The present review describes the role of these enzymes, the status of their molecular characterization and the current advancements in the area of developing inhibitors against each of the enzymes of the pathway.

The role of phosphorylation/dephosphorylation of acetyl-CoA carboxylase in the regulation of mammalian fatty acid biosynthesis

1986 ◽  
Vol 14 (3) ◽  
pp. 559-562 ◽  
Author(s):  
MICHAEL R. MUNDAY ◽  
TIMOTHY A. J. HAYSTEAD ◽  
ROSS HOLLAND ◽  
DAVID A. CARLING ◽  
D. GRAHAME HARDIE
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acetyl Coa Carboxylase ◽  
Acid Biosynthesis ◽  
Acetyl Coa

scholarly journals The role of fatty acid biosynthesis and degradation in the supply of substrates for poly(3-hydroxyalkanoate) formation inPseudomonas putida

1992 ◽  
Vol 103 (2-4) ◽  
pp. 159-163 ◽  
Author(s):  
G. Eggink ◽  
P. Waard ◽  
G.N.M. Huijberts
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acid Biosynthesis

The Role of Tandem Acyl Carrier Protein Domains in Polyunsaturated Fatty Acid Biosynthesis

2008 ◽  
Vol 130 (20) ◽  
pp. 6336-6337 ◽  
Author(s):  
Hui Jiang ◽  
Ross Zirkle ◽  
James G. Metz ◽  
Lisa Braun ◽  
Leslie Richter ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acyl Carrier Protein ◽  
Protein Domains ◽  
Carrier Protein ◽  
Acid Biosynthesis

scholarly journals Functional Role of Linc-RNAs in Multiple Myeloma: Linc-MIR17HG Affects Fatty Acid Biosynthesis Via transcriptional Regulation of ACC1 with Potential Therapeutic Implications

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 1925-1925
Author(s):  
Eugenio Morelli ◽  
Mariateresa Fulciniti ◽  
Mehmet Kemal Samur ◽  
Francesca Scionti ◽  
Annamaria Gulla ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Cell Lines ◽  
Fatty Acid Biosynthesis ◽  
Newly Diagnosed ◽  
Rna Seq ◽  
Acid Biosynthesis ◽  
Transcriptional Regulatory ◽  
Equity Ownership ◽  
Regulatory Functions

Abstract Besides the well described function of RNA to produce proteins, a large volume of transcribed product has non-coding function. A recent analysis of RNA repertoire has identified a family of non-coding transcripts with sequence longer than 200 nucleotides, the long intergenic non-coding RNAs (lincRNAs). Although lincRNAs have been considered to provide regulatory functions, their precise role in cellular biology remains unclear. Using our RNA-seq data from 360 newly-diagnosed patients and 18 normal plasma cells, we have recently described the landscape of lincRNAs in multiple myeloma (MM) and reported their role as independent risk predictors for survival outcome. We have now studied the functional role of a lincRNA, the miR-17-92 primary precursor linc-MIR17HG, present in our lincRNA profile and highly correlated with overall survival in MM. We observe that inhibition of linc-MIR17HG by antisense LNA GapmeRs (n=2) leads to apoptosis in 12 genotypically distinct MM cell lines as well as in 13 primary patient MM cells. These effects are not fully rescued by expression of miR-17-92 microRNAs, suggesting a distinct biological function for linc-MIR17HG in MM. We therefore performed gene expression profile in 2 MM cell lines (AMO1 and NCI-H929) and in 2 primary patient MM cells after short-term suppression of linc-MIR17HG; and, at these early time points (18-36h), we found significant downregulation of a subset of genes (FC>2; p<0.05) including ACC1, EXT1, EPT1, ANO6, CCDC91 and KIA1109, but not miR-17-92 microRNAs. These transcriptional changes were validated by qRT-PCR in MM cell lines and primary MM cells exposed to different LNA GapmeRs targeting linc-MIR17HG with a non-overlapping spectrum of off-target effects. Importantly, our RNA-seq analysis of 360 newly-diagnosed MM patients from IFM/DFCI 2009 clinical study showed that expression of linc-MIR17HG strongly correlated with the expression of each of these genes (R2>0.4; p<0.01) in MM patients, further suggesting a regulatory function by linc-MIR17HG at transcriptional level. Using CRISPR interference (CRISPRi), we have also identified that the linc-MIR17HG with transcriptional regulatory functions is not produced from the canonical transcript isoforms MIR17HG-201/-203; these isoforms, rather, appear to be involved in production of microRNAs, leaving an alternative transcription start site usage as possible source for the transcriptional regulator isoform(s). We next investigated whether regulation of these early targets may contribute to the activity of linc-MIR17HG. We performed a RNAi-based screening in 2 MM cells lines (AMO1 and NCI-H929) by silencing each of the linc-MIR17HG downstream target genes with at least 2 different highly-specific siRNAs. This approach revealed that silencing of acetyl-CoA carboxylase 1 (ACC1, also known as ACACA), a gene encoding the limiting enzyme in the biosynthesis of fatty acids, significantly affects MM cell growth and viability. These results were validated using stable knock-down via shRNAs, confirming ACC1 as a novel vulnerability in MM. These results provide a molecular basis for reported role of fatty acid metabolism in MM cell growth and survival. We have now evaluated two orally available inhibitors of ACC1 activity, ND-630 and ND-646, in a panel of 10 MM cell lines, an report a potent time- and dose-dependent anti-proliferative effect. The activity of these inhibitors and linc-MIR17HG on fatty acid biosynthesis in MM cells is under investigation and will be presented. We have also begun to investigate molecular pathway used by linc-MIR17HG to modulate ACC1 function. Our preliminary data suggest that linc-MIR17HG may function as a scaffold between MYC and the E-box motifs present on ACC1 intronic sequences, facilitating MYC binding and its transcriptional activity. In conclusion, we highlight a transcriptional regulatory activity of a lincRNA in MM with significant functional impact that can be therapeutically translated. Disclosures Anderson: Bristol Myers Squibb: Consultancy; Millennium Takeda: Consultancy; Gilead: Membership on an entity's Board of Directors or advisory committees; OncoPep: Equity Ownership, Other: Scientific founder; C4 Therapeutics: Equity Ownership, Other: Scientific founder; Celgene: Consultancy. Munshi:OncoPep: Other: Board of director.

scholarly journals The Effect of TRANSPARENT TESTA2 on Seed Fatty Acid Biosynthesis and Tolerance to Environmental Stresses during Young Seedling Establishment in Arabidopsis

2012 ◽  
Vol 160 (2) ◽  
pp. 1023-1036 ◽  
Author(s):  
Mingxun Chen ◽  
Zhong Wang ◽  
Yana Zhu ◽  
Zhilan Li ◽  
Nazim Hussain ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Seedling Establishment ◽  
Fatty Acid Biosynthesis ◽  
Environmental Stresses ◽  
Acid Biosynthesis ◽  
Seed Fatty Acid ◽  
Young Seedling
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