Development and validation of gene‐based markers for shrunken2‐Reference allele and their utilization in marker‐assisted sweet corn ( Zea mays Sachharata ) breeding programme

2020 ◽  
Vol 139 (6) ◽  
pp. 1135-1144
Author(s):  
Rashmi Chhabra ◽  
Firoz Hossain ◽  
Vignesh Muthusamy ◽  
Aanchal Baveja ◽  
Brijesh Kumar Mehta ◽  
...  
2019 ◽  
Vol 70 (10) ◽  
pp. 868 ◽  
Author(s):  
Rashmi Chhabra ◽  
Firoz Hossain ◽  
Vignesh Muthusamy ◽  
Aanchal Baveja ◽  
Brijesh K. Mehta ◽  
...  

The sweet corn variety of maize (Zea mays L.) has become popular worldwide. The recessive allele of sugary1 (su1) encoding starch de-branching enzyme has been much used for sweet corn cultivar development. Here, we aimed to develop su1-based functional marker(s) by using six diverse inbred lines of sugary type and five inbred lines of wild type, and using 27 overlapping primers. In total, 12 indels (insertion and deletion) and 96 SNPs (single nucleotide polymorphisms) were identified that clearly differentiated the dominant and recessive allele of su1. Among these, a 36-bp indel (at position 1247) in the promoter region included a TATA-box, and a 6-bp indel (at position 6456) in intron-10 was predicted to have SRp40 exon-splicing enhancer. Nucleotide substitution in exon-2 at position 2703 (SNP-2703) was involved in C to G mutation leading to conversion of phenylalanine to leucine. The 6-bp and 36-bp indels and SNP-2703 were used to develop breeder-friendly codominant markers: SuDel6-FR, SuDel36-FR and SNP2703-CG-85/89. All three markers were validated in five F2 populations, and SuDel36-FR and SNP2703-CG-85/89 were validated in a set of 230 diverse inbreds having both mutant and wild-type alleles of Su1. This is the first report of development and validation of universal functional markers for su1. These markers (SuDel36-FR and SNP2703-CG-85/89) assume great significance in marker-assisted breeding program.


1992 ◽  
Vol 57 (2) ◽  
pp. 454-457 ◽  
Author(s):  
S. ZHU ◽  
J. R. MOUNT ◽  
J. L. COLLINS

1969 ◽  
Vol 95 (1-2) ◽  
pp. 105-110
Author(s):  
James S. Beaver ◽  
Bryan R. Brunner ◽  
Arístides Armstrong
Keyword(s):  
Zea Mays ◽  

RELEASE OF SWEET CORN (ZEA MAYS L.) OPEN-POLLINATED CULTIVAR 'SURESWEET 2011


2013 ◽  
Vol 100 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Levent Genc ◽  
Melis Inalpulat ◽  
Unal Kizil ◽  
Mustafa Mirik ◽  
Scot E. Smith ◽  
...  

2015 ◽  
Vol 67 (3) ◽  
pp. 993-1000 ◽  
Author(s):  
Lydia Shtereva ◽  
Roumiana Vassilevska-Ivanova ◽  
Tanya Karceva

An experiment was carried out hydroponically under laboratory conditions to investigate the effect of salt stress on several physiological and biochemical parameters of three sweet corn (Zea mays L. var. saccharata) genotypes: lines 6-13, C-6 (pollen source) and their heterotic F1 hybrid ?Zaharina?. The degree of salinity tolerance among these genotypes was evaluated at three different sodium chloride (NaCl) concentrations: 0 mM, 100 mM, 125 mM and 150 mM. Seed germination, plant growth and biochemical stress determining parameters such as malondialdehyde (MDA), proline content and hydrogen peroxide (H2O2) levels were compared between seedlings of lines and hybrid. The obtained results indicated that both lines and hybrid have similar responses at different salinity levels for all examined traits. All the seedlings? growth parameters, such as germination percentage, root length, shoot length, root and shoot fresh and dry weight, decreased with increasing salinity level. MDA, proline and H2O2 increased at different saline conditions in comparison to the control. Based on the results, of the three genotypes examined, the hybrid Zaharina, followed by line C-6, was more salt-sensitive than line 6-13 in salt stress condition.


2011 ◽  
Vol 64 ◽  
pp. 142-148 ◽  
Author(s):  
T.K. James ◽  
A. Rahman ◽  
C.R. McGill ◽  
P.D. Trivedi

The wild type of broom corn millet (Panicum miliaceum) is a serious emerging weed currently prevalent in New Zealand sweet corn (Zea mays) crops This study shows its seed is nearly twice the weight of other common grass weeds and can germinate in the temperature range 1634C with 50 germination at 26C and greatest germination occurring at 31C At 15C it took 8 days for seedlings to emerge but required only 4 days at 25C Seed was able to emerge from depths of up to 170 mm in a range of soils Experiments showed that broom corn millet seed can persist in the soil for longer than 2 years in the field but is killed in silage stack and bales Immersion in stock effluent for up to three months reduced seed germination to less than 40 These characteristics are discussed in relation to herbicide and management control options for this weed


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