Variation of PHT families adapts salt cress to phosphate limitation under salinity

2021 ◽  
Author(s):  
Sulian Lv ◽  
Duoliya Wang ◽  
Ping Jiang ◽  
Weitao Jia ◽  
Yinxin Li
Keyword(s):  
Phosphate Limitation ◽  
Salt Cress

scholarly journals Non-Conventional Metal Ion Cofactor Requirement of Dinoflagellate Alkaline Phosphatase and Translational Regulation by Phosphorus Limitation

2019 ◽  
Vol 7 (8) ◽  
pp. 232 ◽  
Author(s):  
Xin Lin ◽  
Chentao Guo ◽  
Ling Li ◽  
Tangcheng Li ◽  
Senjie Lin
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ion ◽  
Translational Regulation ◽  
Organic Phosphorus ◽  
Niche Differentiation ◽  
Phosphorus Limitation ◽  
Marine Phytoplankton ◽  
Phosphate Limitation ◽  
Dissolved Organic Phosphorus ◽  
Metal Cofactor

Alkaline phosphatase (AP) enables marine phytoplankton to utilize dissolved organic phosphorus (DOP) when dissolved inorganic phosphate (DIP) is depleted in the ocean. Dinoflagellate AP (Dino-AP) represents a newly classified atypical type of AP, PhoAaty. Despite While being a conventional AP, PhoAEC is known to recruit Zn2+ and Mg2+ in the active center, and the cofactors required by PhoAaty have been contended and remain unclear. In this study, we investigated the metal ion requirement of AP in five dinoflagellate species. After AP activity was eliminated by using EDTA to chelate metal ions, the enzymatic activity could be recovered by the supplementation of Ca2+, Mg2+ and Mn2+ in all cases but not by that of Zn2+. Furthermore, the same analysis conducted on the purified recombinant ACAAP (AP of Amphidinium carterae) verified that the enzyme could be activated by Ca2+, Mg2+, and Mn2+ but not Zn2+. We further developed an antiserum against ACAAP, and a western blot analysis using this antibody showed a remarkable up-regulation of ACAAP under a phosphate limitation, consistent with elevated AP activity. The unconventional metal cofactor requirement of Dino-AP may be an adaptation to trace metal limitations in the ocean, which warrants further research to understand the niche differentiation between dinoflagellates and other phytoplankton that use Zn–Mg AP in utilizing DOP.

scholarly journals The Phosin PptA Plays a Negative Role in the Regulation of Antibiotic Production in Streptomyces lividans

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 325
Author(s):  
Noriyasu Shikura ◽  
Emmanuelle Darbon ◽  
Catherine Esnault ◽  
Ariane Deniset-Besseau ◽  
Delin Xu ◽  
...  
Keyword(s):  
Antibiotic Production ◽  
Streptomyces Lividans ◽  
Positive Control ◽  
Phosphate Limitation ◽  
Antibiotic Biosynthesis ◽  
Nudix Hydrolase ◽  
Energetic Stress ◽  
Accessory Factor ◽  
Negative Role ◽  
Weak Expression

In Streptomyces, antibiotic biosynthesis is triggered in phosphate limitation that is usually correlated with energetic stress. Polyphosphates constitute an important reservoir of phosphate and energy and a better understanding of their role in the regulation of antibiotic biosynthesis is of crucial importance. We previously characterized a gene, SLI_4384/ppk, encoding a polyphosphate kinase, whose disruption greatly enhanced the weak antibiotic production of Streptomyces lividans. In the condition of energetic stress, Ppk utilizes polyP as phosphate and energy donor, to generate ATP from ADP. In this paper, we established that ppk is co-transcribed with its two downstream genes, SLI_4383, encoding a phosin called PptA possessing a CHAD domain constituting a polyphosphate binding module and SLI_4382 encoding a nudix hydrolase. The expression of the ppk/pptA/SLI_4382 operon was shown to be under the positive control of the two-component system PhoR/PhoP and thus mainly expressed in condition of phosphate limitation. However, pptA and SLI_4382 can also be transcribed alone from their own promoter. The deletion of pptA resulted into earlier and stronger actinorhodin production and lower lipid content than the disruption of ppk, whereas the deletion of SLI_4382 had no obvious phenotypical consequences. The disruption of ppk was shown to have a polar effect on the expression of pptA, suggesting that the phenotype of the ppk mutant might be linked, at least in part, to the weak expression of pptA in this strain. Interestingly, the expression of phoR/phoP and that of the genes of the pho regulon involved in phosphate supply or saving were strongly up-regulated in pptA and ppk mutants, revealing that both mutants suffer from phosphate stress. Considering the presence of a polyphosphate binding module in PptA, but absence of similarities between PptA and known exo-polyphosphatases, we proposed that PptA constitutes an accessory factor for exopolyphosphatases or general phosphatases involved in the degradation of polyphosphates into phosphate.

The response of planktonic phosphate uptake and turnover to ultraviolet radiation in Lake Erie

2002 ◽  
Vol 59 (5) ◽  
pp. 778-786 ◽  
Author(s):  
C D Allen ◽  
R E.H Smith
Keyword(s):  
Ultraviolet Radiation ◽  
Lake Erie ◽  
Radiation Treatment ◽  
Phosphate Uptake ◽  
Ultraviolet B ◽  
Phosphate Limitation ◽  
Bacterial Cells ◽  
Near Surface ◽  
Ambient Concentrations ◽  
Uptake Activity

The hypothesis that ambient ultraviolet radiation (UVR), at near-surface intensities, may diminish phosphorus availability to phytoplankton was tested in Lake Erie in July and August of 1998 and 1999. Relative to samples exposed to photosynthetically active radiation (PAR, 400–700 nm) only, those exposed to ultraviolet-B (UVB, 280–320) and (or) ultraviolet-A (UVA, 320–400 nm) in natural sunlight, or kept in darkness, had diminished phosphate uptake rates at elevated (1 µM P) dissolved phosphate concentrations. By contrast, the specific uptake rate of dissolved phosphate at ambient concentrations (turnover rate) was not significantly affected by UVR or darkness. Turnover was usually dominated by particles smaller than 0.8 µm, whereas uptake from elevated concentrations was dominated by larger particles. The size distribution of turnover and uptake activity was not affected by radiation treatment. Chlorophyll a concentrations were decreased by sufficient exposure to UVB and (or) UVA and increased by deprivation of PAR (dark controls), but the concentration of bacterial cells was unaffected. The results showed that UVR inhibited the phosphate uptake potential of larger, probably algal, plankton but did not change the apparent severity of phosphate limitation at ambient concentrations.

Synergistic response of plant hairy-root cultures to phosphate limitation and fungal elicitation

1991 ◽  
Vol 7 (5) ◽  
pp. 434-438 ◽  
Author(s):  
David S. Dunlop ◽  
Wayne R. Curtis
Keyword(s):  
Hairy Root ◽  
Phosphate Limitation ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Fungal Elicitation ◽  
Synergistic Response

scholarly journals Metabolic Changes of Amino Acids and Flavonoids in Tea Plants in Response to Inorganic Phosphate Limitation

2018 ◽  
Vol 19 (11) ◽  
pp. 3683 ◽  
Author(s):  
Santosh KC ◽  
Meiya Liu ◽  
Qunfeng Zhang ◽  
Kai Fan ◽  
Yuanzhi Shi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Inorganic Phosphate ◽  
Tea Plant ◽  
Phosphate Limitation ◽  
P Limitation ◽  
Metabolic Genes ◽  
Expression Of Genes ◽  
Tea Plants ◽  
Change Response ◽  
Tof Ms

The qualities of tea (Camellia sinensis) are not clearly understood in terms of integrated leading molecular regulatory network mechanisms behind inorganic phosphate (Pi) limitation. Thus, the present work aims to elucidate transcription factor-dependent responses of quality-related metabolites and the expression of genes to phosphate (P) starvation. The tea plant organs were subjected to metabolomics analysis by GC×GC-TOF/MS and UPLC-Q-TOF/MS along with transcription factors and 13 metabolic genes by qRT-PCR. We found P starvation upregulated SPX2 and the change response of Pi is highly dependent on young shoots. This led to increased change in abundance of carbohydrates (fructose and glucose), amino acids in leaves (threonine and methionine), and root (phenylalanine, alanine, tryptophan, and tyrosine). Flavonoids and their glycosides accumulated in leaves and root exposed to P limitation was consistent with the upregulated expression of anthocyanidin reductase (EC 1.3.1.77), leucoanthocyanidin dioxygenase (EC 1.4.11.19) and glycosyltransferases (UGT78D1, UGT78D2 and UGT57L12). Despite the similar kinetics and high correlation response of Pi and SPX2 in young shoots, predominating theanine and other amino acids (serine, threonine, glutamate, valine, methionine, phenylalanine) and catechin (EGC, EGCG and CG) content displayed opposite changes in response to Pi limitation between Fengqing and Longjing-43 tea cultivars.

scholarly journals Seasonal variations in response of periphytic algal community to nutrient enrichment in the river Ganga (Varanasi, India)

2018 ◽  
Vol 54 ◽  
pp. 32 ◽  
Author(s):  
Arpana Yadav ◽  
Dhananjay Kumar ◽  
Ram Sharan Singh ◽  
Lalit K. Pandey ◽  
Jyoti Rai
Keyword(s):  
High Temperature ◽  
Species Diversity ◽  
River Water ◽  
Green Algae ◽  
Nutrient Enrichment ◽  
Seasonal Variations ◽  
Algal Community ◽  
Phosphate Limitation ◽  
River Ganga ◽  
Time Periods

Effect of nutrient enrichment on periphyton was studied using chemical diffusing substrates that released, singly or in combination, PO43−-P and nitrogen, both NO3−-N and NH4+-N. The study was carried out in March and June 2014 as these two time periods showed abundance of different groups of algae. Whereas diatoms and green algae were abundant in March, cyanobacteria dominated in June. Nitrogen was primarily growth limiting in river water as its enrichment enhanced the growth of periphyton. Phosphate limitation was secondary in nature. Enrichment of NH4+-N caused greater enhancement of periphytic growth than that of NO3−-N. Nutrient enrichment reduced species diversity, further enhancing total biovolume of the periphytic group that was predominant at that particular point of time. Nutrient enrichment markedly increased cyanobacterial populations in June due inter alia to high temperature. In spite of nutrient enrichment, the periphytic biomass did not reach the level (chla≥ 100–150 mg m−2) considered as the nuisance, except in March when combined enrichment of PO43−-P and NO3−-N greatly enhanced the periphytic biomass.

Interactions between phosphate and oxidative metabolism in proximal renal tubules

1984 ◽  
Vol 247 (4) ◽  
pp. F575-F581 ◽  
Author(s):  
P. C. Brazy ◽  
L. J. Mandel ◽  
S. R. Gullans ◽  
S. P. Soltoff
Keyword(s):  
Oxidative Metabolism ◽  
Short Chain Fatty Acids ◽  
Phosphate Limitation ◽  
Rabbit Kidney ◽  
Renal Tubules ◽  
Organic Substrates ◽  
Atp Content ◽  
Fluid Absorption ◽  
Consumption Rates ◽  
Free Media

These studies examine the phosphate dependence of fluid absorption and oxidative metabolism in proximal tubules from rabbit kidney. Removal of inorganic phosphate from intraluminal fluid in isolated perfused tubules reduced fluid absorption (Jv) from 1.0 nl X mm-1 X min-1 to zero and removal of phosphate from the medium bathing tubules in suspension reduced O2 consumption rates (coupled, uncoupled, ouabain sensitive, and ouabain insensitive) and ATP contents by 30-40%. Inhibitions induced by removal of phosphate could be altered by addition of selected organic substrates to ambient fluids. Addition of succinate, citrate, or malate preserved uncoupled respiration and partially preserved Jv (0.4-0.9 nl X mm-1 X min-1), with succinate being most effective. Addition of short-chain fatty acids (butyrate or valerate) to phosphate-free media had little or no effect on Jv. n-Butylmalonate, an inhibitor of dicarboxylate anion transport into mitochondria, reduced oxidative metabolism, ATP content, and Jv when added to phosphate-containing media but had no further effect on these tubular functions when added to phosphate-free media. Together these results suggest that phosphate limitation reduces dicarboxylate anion availability to mitochondria and that this inhibition may contribute to the overall reduction in oxidative metabolism and Jv observed under these conditions.

The complete chloroplast genome of salt cress (Eutrema salsugineum)

2015 ◽  
Vol 27 (4) ◽  
pp. 2862-2863 ◽  
Author(s):  
Xinyi Guo ◽  
Guoqian Hao ◽  
Tao Ma
Keyword(s):  
Chloroplast Genome ◽  
Complete Chloroplast Genome ◽  
Salt Cress ◽  
Eutrema Salsugineum
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