Serum myoglobin, creatine kinase, and cell-free DNA in endurance sled dogs and sled dogs with clinical rhabdomyolysis

Purpose: To evaluate whether cell-free DNA (cfDNA) levels increase immediately after an acute light and heavy resistance exercise (RE) bout and whether cfDNA levels are associated with functional muscle capacity up to 48 h after an exercise session. Methods: Twenty healthy volunteers performed 3 sets of leg-press RE with 80% of 1-repetition maximum (1RM) (RE80) or 40% of 1RM (RE40) with similar exercise volume. Blood lactate was measured after completion of the 3 sets. Creatine kinase, cfDNA, and jump performance were evaluated before (pre) exercise, immediately postexercise (post-0h), and every 24 h until 48 h. Results: Lactate concentration increased similarly in both groups (RE40 4.0 [1.3] mmol/L; RE80 4.8 [1.3] mmol/L). No changes were observed in squat-jump and countermovement-jump performance after RE40; however, both jumps remained reduced until 48 h in the RE80 group. Creatine kinase concentration increased post-24h only in the RE80 group (pre 128.8 [73.7] U/L to post-24h 313.8 [116.4] U/L). cfDNA concentration increased post-0h only in the RE80 group (pre 249.8 [82.3] ng/mL to post-0h 406.3 [67.2] ng/mL). There was a negative correlation between post-0h cfDNA concentration and post-24h squat jump (r = −.521; P = .01) and post-0h cfDNA concentration and post-24h countermovement jump (r = −.539; P = .01). Conclusion: cfDNA increases in response to RE intensity even when not performed until exhaustion. cfDNA measured immediately after RE is a promising biomarker for muscle-performance decrement up to 48 h after a RE bout.

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Kinetics of Plasma Cell-Free DNA and Creatine Kinase in a Canine Model of Tissue Injury

Journal of Veterinary Internal Medicine ◽

10.1111/jvim.14901 ◽

2017 ◽

Vol 32 (1) ◽

pp. 157-164 ◽

Cited By ~ 6

Author(s):

I.J. Wilson ◽

R.K. Burchell ◽

A.J. Worth ◽

S.E. Burton ◽

K.R. Gedye ◽

...

Keyword(s):

Creatine Kinase ◽

Plasma Cell ◽

Tissue Injury ◽

Canine Model ◽

Cell Free Dna ◽

Free Dna ◽

Kinetics Of

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The dynamics of cell-free DNA from urine and blood after a full marathon

10.1101/2021.03.06.434188 ◽

2021 ◽

Author(s):

Yasuhiro Shishikura ◽

Katsuyuki Tokinoya ◽

Yuichi Aita ◽

Nanami Sekine ◽

Takehito Sugasawa ◽

...

Keyword(s):

Creatine Kinase ◽

Acute Exercise ◽

Fragment Size ◽

White Blood Cells ◽

Healthy Male ◽

Cell Free Dna ◽

Extracellular Traps ◽

Free Dna ◽

Blood And Urine Samples ◽

Male Subjects

AbstractPurposeCell-free DNA (cfDNA) has been investigated as a minimally invasive biomarker for many diseases, particularly cancer. An increase in cfDNA has been observed during exercise. Neutrophil extracellular traps (NETs) may be the origin of cfDNA in response to acute exercise, but the mechanisms of generation of cfDNA during exercise remain unclear. In this study we investigated the dynamics of serum and urinary cfDNA levels and determined the relevance of other biomarkers to serum and urinary cfDNA levels and fragment size after a full marathon.MethodsSamples were collected from 23 healthy male subjects. Blood and urine samples were collected before and immediately, two hours, and one day after the full marathon. The measurements included serum and urinary cfDNA, creatine kinase, myoglobin, creatinine, white blood cells, platelets, and lactoferrin from blood, and amylase, albumin, and creatinine from urine.ResultsSerum and urinary cfDNA levels increased after a full marathon. Creatine kinase, myoglobin, and creatinine in blood, and albumin and creatinine in urine also increased significantly after a full marathon. Serum cfDNA showed peak values about 180 bp after the full marathon. Values over 1000 bp were present at two hours post-marathon. Urinary cfDNA showed peak values from 35 bp to 50 bp after the full marathon. Values over 1000 bp appeared at Immediately and two hours post marathon.ConclusionThis study revealed that both serum and urinary cfDNA levels transiently increased after a full marathon. In addition, these cfDNA fragment varied in size.

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