BLOOD GLUCOSE LEVEL AND LIPID PROFILE OF ALLOXAN–INDUCED DIABETIC RATS TREATED WITH NA-ALGINATE FROM SEAWEED Turbinaria ornata (Turner) J.Agardh

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Amir Husni ◽  
Setyaning Pawestri ◽  
Alim Isnansetyo
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Glucose Level ◽  
Postprandial Glucose ◽  
Diabetic Rats ◽  
Turbinaria Ornata ◽  
In Vivo Test ◽  
Randomized Design

Diabetes mellitus (DM) is a metabolic disorder due to insulin deficiency, insulin resistance or inability of β-cells of pancreas to produce insulin. This study was aimed to evaluate the effects of Na-alginate from Turbinaria ornata (Turner) J.Agardh on glucose level and lipid profile in alloxan-induced diabetic rats. Na-alginate was extracted from T. ornata then analyzed by TLC and FTIR. In vivo test was performed on alloxan monohydrate induced diabetic rats. In vivo parameters evaluated were body weight, blood glucose and lipid profiles (total cholesterol, HDL-c, LDL-c, and triglyceride). In vivo test was conducted by a complete randomized design with six treatments, 0.5 % CMC-Na, glibenclamide 5 mg kg–1, Na-alginate 200 mg kg–1, Na-alginate 400 mg kg–1, Na-alginate 600 mg kg–1, and NaCl 0.9 % per each five replications. The results showed that Na-alginate at 600 mg·kg–1 increased weight of rat significantly comparing to normal control. Na-alginate 600 mg kg–1also lowered preprandial and postprandial glucose better than other doses. Increasing dose of Na-alginate lowered total cholesterol, increased HDL-c, and lowered LDL-c. The higher dose of alginate gave greater effect on diabetic rats. However, Na-alginate did not affect the triglyceride.

scholarly journals Treatment of Diabetic Condition in Streptozotocin Induced Diabetic Wister Rats Using Food Blends Such as Unripe Plantain, Soybean and Ginger

2019 ◽  
pp. 1-12
Author(s):  
I. Iwanegbe ◽  
M. Suleiman ◽  
A. Jimah
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Cell Volume ◽  
Glucose Level ◽  
Normal Control ◽  
Packed Cell Volume ◽  
Diabetic Rats ◽  
Diabetic Patients

Aims: To investigate the effect of food blends (plantain, soybean and ginger) on the blood glucose, lipid profile and haematological indices on streptozotocin induced diabetic rats. Methodology: A total of 35 rats of mean body weight 219.07 g separated into7 groups (5 per group) where induced by a single intraperitoneal (I.P) injection of streptozotocin (0.1 g dissolved in 5 ml of freshly prepared sodium citrate buffer 0.1 M, pH 4.5) at a dose of 40 mg/kg body weight after fasting for 12 hours and fed with flours/blends. The flours were produced from plant materials for different treatments/blends (blend A=100% unripe plantain, B=80% unripe plantain, 14% soybean, 6% ginger, C=70% unripe plantain, 26% soybean, 4% ginger, D= 60% unripe plantain, 38% soybean, 2% ginger, E= 50% unripe plantain, 50% soybean) and the phytochemicals and minerals content were determined. Blood glucose was determined at 5 days interval for 25 days. Diabetes was confirmed in rats with blood glucose concentrations >200 mg/dl. After 25 days rats were anaesthetized with chloroform vapour and blood samples collected by cardiac puncture for haematology and lipid profile determination. Results: The results showed that unripe plantain, soya beans and ginger in adequate proportion(C=70% unripe plantain, 26% soybean, 4% ginger or D= 60% unripe plantain, 38% soybean, 2% ginger) could help to reduce blood glucose, improve haematological parameters and lipid profile. Significant reduction was observed in the blood glucose level of rats fed blends C and D from 286 to 85 mg/dl and 307 to 90 mg/dl respectively at the end of experiment. These results also demonstrated that the inclusion of ginger at 6% causes rise in blood glucose level. Total cholesterol (TC) increased in all the blends. However, the lowest concentration of TC was observed in blends C and D. The highest packed cell volume (60%) and Haemoglobin (20 g/dl) level observed in rats fed blend C was significantly higher than the normal control fed conventional feeds. The increase in packed cell volume (PCV) (50%) and Hb (17 g/dl) in diabetic rats demonstrated that the formulated blend C was able to raise PCV and Hb above 50% and 17 g/dl (Normal control NC) respectively. Significant increase (P<0.05) in low density lipoprotein cholesterol (LDLc) was also observed in all the blends with blend C having the least (4.0 mg/dl) close to NC (2.0 mg/dl). Conclusion: From the results it is evident that blend C will manage and improve the health status of diabetic patients.

scholarly journals Bioequivalence Assessment of Metformin Tablet Formulations of Bangladesh

2014 ◽  
Vol 6 (3) ◽  
pp. 581-588
Author(s):  
M. A. Islam ◽  
A. Islam ◽  
M. R. I. Khan ◽  
R. Sharmin ◽  
M. I. I. Wahed ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Diabetic Rats ◽  
In Vivo Evaluation ◽  
Glucose Levels ◽  
Drug Content ◽  
Oral Antidiabetic ◽  
Metformin Hcl

Six marketed oral antidiabetic metformin tablets in Bangladesh have been studied for their drug content, release profile and glucose lowering capacities. This sort of study is a good indicator for in vivo evaluation of the quality of an oral antidiabetic preparation. Marketed preparations of metformin-HCl from different manufacturers were randomly chosen for this study. The drug content was within the United State Pharmacopoeia (USP) specified limit (95-105%) in all cases. The blood glucose levels were investigated in streptozotocin-induced diabetic rats (SIDRs) after 5 hours of single dose (110 mg/kg body weight) treatment of the products; significantly (p<0.05) reduced blood glucose level by 58.1, 53.2, 50.8, 77.0, 72.9 and 49.1%, respectively; which were consistent with antihyperglycemic effects of standard metformin-HCl (71.3%). All the products were found to be qualified in lowering blood glucose level. It may be inferred that the metformin-HCl tablets of Bangladeshi manufacturers complies with the standard specifications for drug contents, dissolution and antihyperglycemic properties.© 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.19290 J. Sci. Res. 6 (3), 581-588 (2014)

scholarly journals In vivo and in vitro antidiabetic properties of alkaloids extract from Salvia chudaei

2021 ◽  
pp. 45-53
Author(s):  
Mohammed Tlili ◽  
Roukia Hammoudi ◽  
Mahfoud Hadj-Mahammed
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Kidney Function ◽  
Inhibitory Effect ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Liver And Kidney ◽  
Mean Blood Glucose

The aim of this study was to evaluate, for the first time, the antidiabetic effect of the alkaloids extract of Salvia chudaei Batt. & Trab. (Lamiaceae) on alloxan-induced diabetic rats. The alkaloids extract was prepared, and the in vitro inhibitory effect of key digesting enzymes related to postprandial hyperglycemia were determined. After acute toxicity test, the Swiss albino rats were induced with alloxan to get experimental diabetes animals. The fasting mean blood glucose, lipid profile, different liver and kidney function biomarkers and antioxidant biomarkers levels, after treatment for 30 days, diabetic untreated and diabetic rats treated with alkaloids extract were estimated. The alkaloids displayed remarkable in inhibiting ?-glucosidase (IC50 = 248.25?2.61 ?g/ml) than ?-amylase (IC50 = 262.96?9.64 ?g/ml) activities. In vivo, the results proved that alkaloids extract at dose of 500 mg/kg bw decreased significantly the blood glucose, lipid profile levels and improved the liver and kidney function biomarkers and increased the activities of antioxidant enzymes (superoxide dismutase and gluthatione reductase). This study demonstrates, that alkaloids are effective in inhibiting hyperglycemia and oxidative stress caused by diabetes.

scholarly journals The Antioxidant and Antihyperglycemic Activities of Bottlebrush Plant (Callistemon lanceolatus) Stem Extracts

Medicines ◽  
2020 ◽  
Vol 7 (3) ◽  
pp. 11 ◽  
Author(s):  
Ramesh Kumar ◽  
Ashutosh Gupta ◽  
Amit Kumar Singh ◽  
Anupam Bishayee ◽  
Abhay K. Pandey
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Metal Ion ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Diabetic Rats ◽  
Health Concern ◽  
Phytochemical Analysis

Background: Diabetes mellitus, a metabolic disease, is a major health concern today throughout the world. Callistemon lanceolatus (Myrtaceae), commonly known as bottlebrush, has been used by Indian tribal communities for the treatment of many diseases. The purpose of this study was to explore antioxidant and antihyperglycemic potential of methanolic and aqueous extracts of the stem of C. lanceolatus in vitro and in vivo. Methods: Phytoconstituents of C. lanceolatus stem were extracted in methanol and water sequentially followed by phytochemical analysis. The in vitro antioxidant potential of aqueous and methanolic extracts was assessed by metal ion chelating, free radical scavenging, and reducing power assays. The in vivo antihyperglycemic activity of the oral methanolic extract was studied in alloxan-induced diabetic rats. Bodyweight and blood glucose were monitored regularly. After the treatment period, serum was examined for total cholesterol, triglycerides, high-density lipoprotein (HDL), bilirubin, creatinine, urea, glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT), and alkaline phosphatase (ALP). Results: Methanolic extract exhibited superior antioxidant activity to aqueous extract. A marked increase in levels of serum markers, viz., glucose, triglycerides, total cholesterol, bilirubin, urea, creatinine, SGOT, SGPT, and ALP along with a reduction in HDL was observed in diabetic rats. Methanol extract treatment for 28 days accounted for a decrease in blood glucose and other metabolic markers accompanied by an improvement in body weight and HDL level in hyperglycemic rats. Conclusions: The present study suggests that C. lanceolatus methanolic stem extract possesses antioxidant and antihyperglycemic activities and has potential as a therapeutic agent in diabetes.

scholarly journals Antidiabetic Activity ofAcacia tortilis(Forsk.) Hayne ssp. raddiana Polysaccharide on Streptozotocin-Nicotinamide Induced Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Pradeep Kumar Bhateja ◽  
Randhir Singh
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Total Cholesterol ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Fasting Blood Glucose Level

The present study was designed to investigate the antidiabetic activity of aqueous extract ofAcacia tortilispolysaccharide (AEATP) from gum exudates and its role in comorbidities associated with diabetes in STZ-nicotinamide induced diabetic rats. Male albino Wistar rats were divided into control, diabetic control, glimepiride treated (10 mg/kg), and diabetic rats treated with 250, 500, and 1000 mg/kg dose of AEATP groups and fasting blood glucose, glycated hemoglobin, total cholesterol, triglyceride, LDL, VLDL, HDL, SGOT, and SGPT levels were measured. STZ significantly increased fasting blood glucose level, glycated hemoglobin, total cholesterol, triglyceride, LDL, VLDL, SGOT, and SGPT levels, whereas HDL level was reduced as compared to control group. After 7 days of administration, 500 and 1000 mg/kg dose of AEATP showed significant reduction(P<0.05)in fasting blood glucose level compared to diabetic control. AEATP has also reduced total cholesterol, triglyceride, LDL, VLDL, SGOT, and SGPT levels and improved HDL level as compared to diabetic control group. Our study is the first to report the normalization of fasting blood glucose level, lipid profile, and liver enzyme in AEATP treated diabetic rats. Thus, it can be concluded that AEATP may have potentials for the treatment of T2DM and its comorbidities.

scholarly journals Effects of Ganoderma lucidum Extract on Diabetic Rats

2018 ◽  
Vol 10 (3) ◽  
pp. 642-647 ◽  
Author(s):  
Nuniek Ina Ratnaningtyas ◽  
Hernayanti Hernayanti ◽  
Suci Andarwanti ◽  
Nuraeni Ekowati ◽  
Endang Sri Purwanti ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Ganoderma Lucidum ◽  
Glycosylated Hemoglobin ◽  
Insulin Level ◽  
Negative Control ◽  
Diabetic Rats ◽  
Treatment Groups ◽  
Randomized Design

Diabetes mellitus (DM) is a metabolic syndrome which occurs when insulin is insufficiently produced or insulin cannot well serve its function. Diabetes is marked with increase in blood glucose level followed by increase in glycosylated hemoglobin level and decrease in insulin level. This research aims to examining the effect of Ganoderma lucidum extract on the blood glucose, insulin and glycosylated hemoglobin (HbA1c) level of diabetic white rat and determining the most effective dose of extract to be a diabetic agent. This research was experimentally conducted by employing Completely Randomized Design (CRD) with 6 treatments and 4 repetitions. The treatment groups consisted of healthy rats group (K1), rats with diabetes as negative control (K2), diabetic rats with the administration of metformin 45 mg/kg BW as the comparison (K3) and diabetic rats with the administration of G. lucidum extract with dose of 250, 500 and 1000 mg /kg BW (K4, K5 and K6 respectively). Blood glucose level examination was conducted after the alloxan induction with single dose of 125 mg/kg BW by intraperitoneal injection. The results show that mushroom G. lucidum extract administration with dose of 1000 mg/kg BW (K6) is the best dose to be an anti-diabetic agent. The benefit of the research is developing anti-diabetic agent from herbal resources.

scholarly journals DESIGN, SYNTHESIS AND PRELIMINARY PHARMACOLOGICAL EVALUATION OF NEW METFORMIN DERIVATIVES

2016 ◽  
Vol 9 (1) ◽  
pp. 239
Author(s):  
Monther F. Mahdi ◽  
Inam S. Arif ◽  
Najwan K. Jubair
Keyword(s):  
Amino Acids ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Glycolic Acid ◽  
Methyl Esters ◽  
Diabetic Rats ◽  
Preliminary Evaluation ◽  
Design Synthesis

<p><strong>Objective: </strong>The purpose of this work was to enhance oral bioavailability of metformin by incorporation of different amino acid residues through the glycolic acid spacer.</p><p><strong>Methods: </strong>Two series of metformin derivatives (V a-e) and (VI a-e) were synthesized by incorporation of five amino acids (glycine, alanine, phenylalanine, proline and GABA amino butyric acid) and their methyl esters respectively into metformin through glycolic acid spacer and preliminary evaluation for the antihyperglycemic activity was carried out using streptozotocin-induced diabetic rats model.</p><p><strong>Results: </strong><em>In vivo</em> anti-hyperglycemic activity of the final compounds (V a-e) and (VI a-e) showed that compounds (V b, V c, VI c and V e) produced higher percent of decrease in blood glucose level compared to metformin after 5 h of the treatment while compounds (VI b, VI c, V d, VI d, V e and VI e) showed profound effect after 24 h of the treatment. Although compounds (V a, VI a, V b and V c) showed a significant decrease in blood glucose level at 5 h but their effect diminished at 24 h. Compound (V e) showed higher anti-hyperglycemic effect than metformin and its effect continued up to 24 h.</p><p><strong>Conclusion: </strong>From this study, it was concluded that incorporation of these amino acids or their methyl esters maintained or enhanced the antihyperglycemic effect of metformin. </p>

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