scholarly journals Allele and genotype frequencies of milkprotein kappa-casein (CSN3) in artifical insemination bulls of Czech Fleckvieh and Holstein breed

2007 ◽  
Vol 55 (5) ◽  
pp. 17-22 ◽  
Author(s):  
Jiří Bezdíček
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Genetic Variability ◽  
Artificial Insemination ◽  
Allele Frequencies ◽  
The Czech Republic ◽  
Holstein Breed ◽  
Genotype Frequencies ◽  
Holstein Bulls

This thesis was meant to evaluate allele and genotype frequencies of kappa-casein (CSN3) in artificial insemination bulls in the Czech Republic of Czech Fleckvieh breed (n = 133) and Holstein breed (n = 58). Studied bulls were born between 1990 and 2005. Calculation was made by PowerMarker (Liu K., Muse S.V. 2005). Genotype frequencies established in Czech Fleckvieh bulls were AA = 0.391 AB = 0.4887 AE = 0.0075 BB = 0.0902 BE = 0.0226 and EE = 0 and allele frequencies were A = 0.6391 B = 0.3459 E = 0.015. Genotype frequencies established in Holstein bulls were AA = 0.5517 AB = 0.2759 AE = 0.1207 BB = 0 BE = 0.0345 and EE = 0.0172 and allele frequencies A = 0.75 B = 0.1552 and E = 0.0948. In Holstein breed we found higher frequency of E allele and higher frequency of genotypes with this allele. Genetic diversity, heterozygosis and PIC values in Czech Fleckvieh Bulls were following 0.4717; 0.5188 and 0.3737 and 0.4044; 0.4310 and 0.3668 in Holstein Bulls. These results show the higher genetic variability in bulls of Czech Fleckvieh breed. Results of χ2 test (Czech Fleckvieh 4.58; Holstein 2.55) show genetical disequilibrium in the Bulls both examined breeds used in artificial insemination in the Czech Republic.

scholarly journals Polymorphism of prolamin proteins of the grain of triticale varieties certifiedin the Czech Republic

2011 ◽  
Vol 51 (No. 4) ◽  
pp. 151-155 ◽  
Author(s):  
T. Vyhnánek ◽  
J. Bednář
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Cold Resistance ◽  
The Czech Republic ◽  
Baking Quality ◽  
The Individual

 Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of prolamin proteins using the PAGE ISTA method. The polymorphism of prolamin proteins allowed the differentiation of the individual triticale varieties in 2002 and 2003 harvests. On the basis of Dice’s calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld 1B3, which is the marker of rye translocation T1BL.1RS, gene Sr31 with resistance to black rust, higher cold resistance and lower baking quality of the wheat. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic.

Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic

2008 ◽  
Vol 124 (2) ◽  
pp. 363-368 ◽  
Author(s):  
M. Hassan ◽  
G. Gomez ◽  
V. Pallás ◽  
A. Myrta ◽  
P. Rysanek
Keyword(s):  
Czech Republic ◽  
Genetic Variability ◽  
Simultaneous Detection ◽  
Stone Fruit ◽  
The Czech Republic

Microsatellite-based genetic diversity and differentiation of foreign common carp (Cyprinus carpio) strains farmed in the Czech Republic

Aquaculture ◽  
2010 ◽  
Vol 298 (3-4) ◽  
pp. 194-201 ◽  
Author(s):  
Martin Hulak ◽  
Vojtech Kaspar ◽  
Klaus Kohlmann ◽  
Kevin Coward ◽  
Jakub Tešitel ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
The Czech Republic ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals Characterization of Czech hop (Humulus lupulus L.) genotypes by molecular methods

2011 ◽  
Vol 48 (No. 8) ◽  
pp. 343-350 ◽  
Author(s):  
J. Patzak
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Humulus Lupulus ◽  
Quality System ◽  
Molecular Methods ◽  
Germplasm Management ◽  
Genetic Studies ◽  
The Czech Republic ◽  
Humulus Lupulus L

In the last year, new hybrid hop (Humulus lupulus L.) variety Agnus was released for cultivation in the Czech Republic. It has been necessary to prepare the quality system of Agnus identification from other Czech genotypes and characterise the germplasm of this variety by molecular methods. We proved that utilization of five STS primer combinations successfully and completely identified and determined Czech released varieties and new promising breeding materials. The use of STS method was also very effective and sensitive for control of authenticity and purity of variety Agnus in multipropagation cycle. The study of genetic diversity of 61 hop varieties by RAPD, STS, ISSR and AFLP methods confirmed, that germplasm of variety Agnus has ranked among high-alfa varieties. The results can be successfully used for identification, germplasm management, genetic studies and breeding purposes by breeders, multipropagators and hop growers.

Genetic diversity of Cryptosporidium spp. including novel identification of the Cryptosporidium muris and Cryptosporidium tyzzeri in horses in the Czech Republic and Poland

2015 ◽  
Vol 114 (4) ◽  
pp. 1619-1624 ◽  
Author(s):  
Pavla Wagnerová ◽  
Bohumil Sak ◽  
John McEvoy ◽  
Michael Rost ◽  
Agniezska Perec Matysiak ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
The Czech Republic ◽  
Cryptosporidium Spp ◽  
Cryptosporidium Muris

scholarly journals Polymorphisms in CSN3, CSN2 and LGB Genes and Their Relation to Milk Production in Dairy Cattle in the Czech Republic

2019 ◽  
Vol 67 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Jindřich Čítek ◽  
Lenka Hanusová ◽  
Lucie Lískovcová ◽  
Eva Samková ◽  
Oto Hanuš ◽  
...  
Keyword(s):  
Czech Republic ◽  
Dairy Cattle ◽  
Protein Content ◽  
Milk Production ◽  
Milk Yield ◽  
Protein Production ◽  
Allele Frequencies ◽  
The Czech Republic ◽  
Associative Analysis ◽  
Genotype And Allele Frequencies

The principal objective of this study was to perform genotyping of the CSN3, CSN2 and LGB genes in selected dairy cattle breeds in the Czech Republic, to determine genotype and allele frequencies and to perform an associative analysis on milk yield in kg, the percentage of fat and protein content, and fat and protein production in kg. The AA was the most frequent genotype for CSN3 with a frequency of 0.474 and the A allele (0.694). The BB genotype (0.814) and the B allele (0.895) predominated in CSN2 gene. Other genotyping was performed for the A1 and A2 alleles, the most frequent were the A2A2 genotype (0.475) and the A2 allele (0.680). The heterozygous AB genotype with a frequency of 0.900 was the most frequent in the LGB gene. A statistically significant higher protein content was found in the AB genotype in comparison to the BB in gene for CSN2. In the remaining cases, the effect of CSN3, CSN2 and LGB polymorphic variations on the milk production indicators was not statistically significant.

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