scholarly journals Antihistamine activity of extracts prepared from buffy-coat layer of horse blood and from oak gall

1960 ◽  
Vol 154 (3) ◽  
pp. 461-478 ◽  
Author(s):  
W. Feldberg ◽  
B. A. Kovacs
Keyword(s):  
Buffy Coat ◽  
Antihistamine Activity ◽  
Horse Blood ◽  
Coat Layer

scholarly journals Liquid Platelet-Rich Fibrin and Heat-Coagulated Albumin Gel: Bioassays for TGF-β Activity

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3466
Author(s):  
Zahra Kargarpour ◽  
Jila Nasirzade ◽  
Layla Panahipour ◽  
Richard J. Miron ◽  
Reinhard Gruber
Keyword(s):  
Target Genes ◽  
Nuclear Translocation ◽  
Kinase Inhibitor ◽  
Plasma Layer ◽  
Buffy Coat ◽  
Type I ◽  
Platelet Rich Fibrin ◽  
Nadph Oxidase 4 ◽  
Coat Layer ◽  
The Impact

Liquid platelet-rich fibrin (PRF) can be prepared by high centrifugation forces separating the blood into a platelet-poor plasma (PPP) layer and a cell-rich buffy coat layer, termed concentrated PRF (C-PRF). Heating the liquid PPP was recently introduced to prepare an albumin gel (Alb-gel) that is later mixed back with the concentrated liquid C-PRF to generate Alb-PRF. PRF is a rich source of TGF-β activity; however, the overall TGF-β activity in the PPP and the impact of heating the upper plasma layer remains unknown. Here, we investigated for the first time the in vitro TGF-β activity of all fractions of Alb-PRF. We report that exposure of oral fibroblasts with lysates of PPP and the buffy coat layer, but not with heated PPP, provoked a robust increase in the TGF-β target genes interleukin 11 and NADPH oxidase 4 by RT-PCR, and for IL11 by immunoassay. Consistent with the activation of TGF-β signaling, expression changes were blocked in the presence of the TGF-β receptor type I kinase inhibitor SB431542. Immunofluorescence and Western blot further confirmed that lysates of PPP and the buffy coat layer, but not heated PPP, induced the nuclear translocation of Smad2/3 and increased phosphorylation of Smad3. The immunoassay further revealed that PPP and particularly BC are rich in active TGF-β compared to heated PPP. These results strengthen the evidence that not only the cell-rich C-PRF but also PPP comprise a TGF-β activity that is, however, heat sensitive. It thus seems relevant to mix the heated PPP with the buffy coat C-PRF layer to regain TGF-β activity, as proposed during the preparation of Alb-PRF.

scholarly journals Relative Centrifugal Force (RCF; G-Force) Affects the Distribution of TGF-β in PRF Membranes Produced Using Horizontal Centrifugation

2020 ◽  
Vol 21 (20) ◽  
pp. 7629
Author(s):  
Zahra Kargarpour ◽  
Jila Nasirzade ◽  
Layla Panahipour ◽  
Richard J. Miron ◽  
Reinhard Gruber
Keyword(s):  
Centrifugal Force ◽  
High Speed ◽  
Target Genes ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Buffy Coat ◽  
Platelet Poor Plasma ◽  
Nadph Oxidase 4 ◽  
Relative Centrifugal Force ◽  
Coat Layer

Solid platelet-rich fibrin (PRF) is produced with centrifugation tubes designed to accelerate clotting. Thus, activated platelets may accumulate within the fibrin-rich extracellular matrix even before centrifugation is initiated. It can thus be assumed that platelets and their growth factors such as transforming growth factor-β (TGF-β) are trapped within PRF independent of their relative centrifugal force (RCF), the gravitation or g-force. To test this assumption, we prepared PRF membranes with tubes where clotting is activated by a silicone-coated interior. Tubes underwent 210 g, 650 g and 1500 g for 12 min in a horizontal centrifuge. The respective PRF membranes, either in total or separated into a platelet-poor plasma and buffy coat fraction, were subjected to repeated freeze-thawing to prepare lysates. Gingival fibroblasts were exposed to the PRF lysates to provoke the expression of TGF-β target genes. We show here that the expression of interleukin 11 (IL11) and NADPH oxidase 4 (NOX4), and Smad2/3 signaling were similarly activated by all lysates when normalized to the size of the PRF membranes. Notably, platelet-poor plasma had significantly less TGF-β activity than the buffy coat fraction at both high-speed protocols. In contrast to our original assumption, the TGF-β activity in PRF lysates produced using horizontal centrifugation follows a gradient with increasing concentration from the platelet-poor plasma towards the buffy coat layer.

scholarly journals Production of Nucleophagocytosis by Rabbit Antileukocytic Serum

Blood ◽  
1953 ◽  
Vol 8 (7) ◽  
pp. 651-654 ◽  
Author(s):  
HYMAN J. ZIMMERMAN ◽  
JOHN R. WALSH ◽  
PAUL HELLER
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Buffy Coat ◽  
Rabbit Serum ◽  
Neutrophilic Polymorphonuclear Leukocytes ◽  
Coat Layer ◽  
Granulocytic Leukemia

Abstract Dried buffy coat layer of the blood from a patient with granulocytic leukemia was injected into a rabbit. Serum from this rabbit, when mixed with the buffy coat layer of the blood from the same patient, resulted in the production of nucleophagocytosis. The serum of the rabbit also showed the ability to produce marked conglutination and destruction of the neutrophilic polymorphonuclear leukocytes.

scholarly journals Prospective Qualitative Study Concerning the Efficiency of PBSC Collections after a Change of Apheresis Device

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5834-5834
Author(s):  
Marie Y. Detrait ◽  
Stephane Morisset ◽  
Jean-Pierre Delville ◽  
Arnaud Lixon ◽  
Julie Boul ◽  
...  
Keyword(s):  
Stem Cell ◽  
Prospective Study ◽  
Peripheral Blood Stem Cell ◽  
Disease Status ◽  
Buffy Coat ◽  
Allogeneic Hsct ◽  
New Device ◽  
Healthy Donors ◽  
Cd34 Cells ◽  
Coat Layer

Abstract Background Efficiency measurement is an important indicator to improve quality of PBSC harvests. We have recently changed our apheresis device for PBSC collections: from the Cobe Spectra (Terumo BCT) to the Optia Spectra (Terumo BCT). We have further to this change decided to follow prospectively the harvests to estimate the efficiency of the new apheresis device. Material and Method Between November 2013 and April 2014, 21 consecutive harvests were conducted on Optia Spectra for ten consecutive patients/healthy donors. Six patients (3 multiple myeloma and 3 NHL) gave autologous PBSC. They were all mobilized with filgrastim (Neupogen, Amgen) which was associated with plerixafor (Mozobil, Genzyme) for two patients and pegfilgrastim (Neulasta, Amgen) for one patient. Four healthy donors gave PBSC for allogeneic HSCT; three were mobilized with filgrastim (Neupogen, Amgen) and one was mobilized with lenograstim (Granocyte, Chugai). Results The data of our 21 consecutive harvests showed a median PB CD34+ count of 20/mL [range, 7-132] and a median collection of 1.94 CD34 +/kg [range, 0.48-8.33]. The correlation between the PB CD34+ count and the number of CD34+ collected was strong (R2=0.83) (fig.1). A median of 3 and 2 collections were necessary in order to obtain a suitable graft for autologous and allogeneic HSCT respectively. In this study, the efficiency of leukapheresis was only calculated for harvests with a PB CD34+ count > or = 20/µL (n=11), yielding a median efficiency of 30% (range, 13-52). These results were compared with those of our historic cohort of harvests achieved with the Cobe Spectra apheresis machine which yielded a median efficiency of 50% [range, 20-70]. In May 2014, in view of our lower efficiency results with the new device after 6 months of prospective study, we asked the Terumo BCT Company for an analysis of the harvests' data recorded in the Optia Spectra apheresis machine. This analysis revealed a systematic accumulation of the buffy coat layer during the procedures, which led to the low efficiency of our harvests. Awareness of the fast accumulation of the buffy coat layer in the collection chamber, especially when the donor's white blood cell count is > 30 G/L, is of utmost importance. The correction has to be made quickly by reducing the depth of collection from 60 to 40 (or 40 to 30) and by decreasing the speed of the pump to 3mL/min if the initial speed is > 3 mL/min. Theoretically, these two operations should allow the elimination of the buffy coat layer and improve the efficiency of the harvests. The outcomes of our patients are described in Table 1. Conclusion After 6 months of prospective study, the median efficiency of our PBSC harvests with the new device was 30%, that is below our expected value. Repeated procedures were necessary to collect enough CD34+ cells for grafting. For this reason, we requested that Terumo BCT make a retrospective analysis of the collection data recorded in the Optia Spectra system. Their audit showed a systematic accumulation of the buffy coat layer in the collection chamber. Now corrective measures have been implemented and we are going to continue this study over the next 6 months (June 2014 - November 2014) to estimate our new PBSC collections' efficiency. Figure 1 Correlation between CD34+ cells collected and CD34+ peripheral blood stem cell (PB) Count (R2= 0.83) Figure 1. Correlation between CD34+ cells collected and CD34+ peripheral blood stem cell (PB). / Count (R2= 0.83) Abstract 5834 Table 1 Patients outcome analysis n Nber of collec- tion(s) CD34+ collected Disease Auto/alloHSCT Aplastic outgoing delayed (neutro or plat) Disease Status at+3Months Disease Status at +6Months alive at Last follow-up<!> Cause of death 1 4 3.46 MM auto No PR PR Yes <! 2 2 6.82 NHL auto No CR NE Yes 3 3 8.21 NHL auto No CR CR Yes 4 2 5.65 MM auto No NE NE Yes 5 (1) 8.33 MDS allo No CR CR Yes 6 (2) 4.31 ALL allo No relapse NE No Relapse 7 (2) 2.1 AML allo Yes (Plat) relapse CR Yes 8 (2) 4.6 MF + AML allo Yes (Plat) NE NE No Relapse 9 4 1.03 MM AutoNot realized NE NE NE Yes 10 1 4.09 MM auto No PR PR Yes Disclosures No relevant conflicts of interest to declare.

Ultrastructure of rat alveolar macrophages activated in situ by poly:IC

1987 ◽  
Vol 45 ◽  
pp. 768-769
Author(s):  
A. M. Klinkner ◽  
R. A. Weiss ◽  
A. Kelley ◽  
P. J. Bugelski
Keyword(s):  
Venous Blood ◽  
Intratracheal Instillation ◽  
Buffy Coat ◽  
Fischer 344 Rats ◽  
Blood Monocytes ◽  
Fischer 344 ◽  
Polyinosinic:Polycytidylic Acid ◽  
Macrophage Activator ◽  
Endogenous Peroxidase

Polyinosinic:polycytidylic acid is an inducer of interferon and a macrophage activator. We have found that intratracheal instillation of polyI:C (IT-pI:C) activates rat bronchoalveolar lavage cells (BAL) for a variety of functions. Examination of Giemsa stained, cytocentrifuge preparations showed that IT-pI:C induced a population of BAL not seen in resident BAL. The morphology of these cells suggested that they might be derived from blood monocytes. To test this hypothesis we have examined several populations of macrophages that had been stained for endogenous peroxidase activity as a marker of cells derived from the monocyte-macrophage lineage.Macrophages were obtained from Fischer 344 rats. Peritoneal exudate cells (PEC) were collected by lavage 4 days after i.p. injection of 20 ml 3% thioglycolate. Buffy coat monocytes were separated from venous blood from naive rats.

Buffy Coat

2020 ◽  
Author(s):  
Keyword(s):  
Buffy Coat

[ID 37205] PACIENTE CRÍTICO: SEGURANÇA EM TERAPIA TRANSFUSIONAL MEDIANTE LISTA DE VERIFICAÇÕES

2019 ◽  
Vol 23 (4) ◽  
Author(s):  
Amanda Paula Gurgel ◽  
Veronica Silva de Melo ◽  
Janaina Silva Leitão ◽  
Rita Monica Borges Studart ◽  
Isabela Melo Bonfim ◽  
...  
Keyword(s):  
Buffy Coat ◽  
Windows Xp

Objetivo: Avaliar a segurança do paciente crítico em terapia transfusional por meio de uma lista de verificações. Material e Métodos: Estudo observacional transversal, com abordagem quantitativa. A pesquisa foi realizada na Unidade Pós-operatória de Alta Complexidade (UPAC) de um hospital público terciário, localizado em Fortaleza-Ceará. As variáveis do estudo foram: idade, sexo, tipo de transplante, período de transfusão, hemocomponente transfundido, registro e horário dos sinais vitais, - Registro da quantidade de bolsas infundidas, Registro/evolução de hemovigilância, Reação transfusional, Etiqueta de identificação do hemocomponente, registro do recebimento do hemocomponente e do responsável pela transfusão. Os resultados foram organizados em tabelas no programa Word do Windows XP Profissional, interpretados e fundamentados com base na literatura pertinente à temática. Resultados: 53,6% transfusões do sexo masculino, seguido de 46,3% do sexo feminino. Hemocomponentes transfundidos: concentrado de hemácias (38,1%), plasma (33,6%), concentrado de plaquetas (15,4%), crioprecipitado (10%) e buffy coat (2,7%). Registro dos sinais vitais: temperatura 62,7%, pressão arterial: 68,2%, frequência cardíaca: 56,4% e frequência respiratória em 57,3%. Em relação ao tempo do registro dos sinais vitais, 47,3% antes do início da transfusão, 20,9% tinham registro 15 minutos após o início e 30,9% ao término.106 transfusões apresentavam registro da quantidade de bolsas infundidas. Registro de hemovigilância: 17,3%. 1 reação transfusional. Identificação do hemocomponente: 87,3%. Registro do recebimento da bolsa: 25,5%. Registro do responsável pelo procedimento: 75,5%. Conclusão: Verifica-se a necessidade de reforçar a importância dos registros por parte dos enfermeiros na unidade em que foi realizado o estudo. DESCRITORES: Enfermagem. Transfusão sanguínea. Segurança do paciente.

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