Freeform Vertical and Horizontal Fabrication of Alginate-Based Vascular-Like Tubular Constructs Using Inkjetting

2014 ◽  
Vol 136 (6) ◽  
Author(s):  
Changxue Xu ◽  
Zhengyi Zhang ◽  
Kyle Christensen ◽  
Yong Huang ◽  
Jianzhong Fu ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Building Blocks ◽  
Important Indicator ◽  
Freeform Fabrication ◽  
Cellular Spheroids ◽  
On Demand ◽  
Extra Effort ◽  
Drop On Demand ◽  
Organ Printing ◽  
Supporting Material

Organ printing, among different tissue engineering innovations, is a freeform fabrication approach for making three-dimensional (3D) tissue and organ constructs using cellular spheroids or bioinks as building blocks. The capability to fabricate vascular-like tubular constructs is an important indicator of the overall feasibility of envisioned organ printing technology. In this study, vascular-like alginate tubes, which mimic typical vascular constructs, are fabricated both vertically and horizontally using drop-on-demand (DOD) inkjetting. Manufacturing-related challenges are different for the vertical and horizontal printing configurations. In general, the vertical printing configuration has instability or collapse/buckling problems and may experience some difficulty in fabricating complex constructs such as Y- or K-shaped constructs if there is no supporting material. The horizontal printing configuration may easily result in a deformed hollow cross section and may require extra effort to mitigate the undesired deformation. It is envisioned that the combination of vertical and horizontal printing provides an efficient and effective way to fabricate complex tubular constructs with both vertical and horizontal branching features.

Drop-on-Demand: A Scale Analysis

2013 ◽  
Author(s):  
Jean-Marc Delhaye ◽  
Nicole Coutris ◽  
Leigh Herran
Keyword(s):  
Complex Fluids ◽  
Scale Analysis ◽  
Efficient Tool ◽  
On Demand ◽  
Low Viscosity ◽  
Drop On Demand ◽  
Organ Printing ◽  
Complex Fluid ◽  
Physical Phenomena ◽  
Liquid Filament

An increasing number of applications deal with dispensing of micro-droplets. For several years the fluids used were mostly Newtonian with low viscosity but there is nowadays a tendency to use more complex fluids. Some recent applications are encountered in the pharmaceutical industry where there is a need of producing on-demand identical droplets of a given size that can be used for drug delivery and in bioengineering where droplets have to contain cells used for tissue manufacturing or organ printing. The objective of the paper is to show that scale analysis is an efficient tool to classify the physical phenomena contributing to the breakup of a jet or of a liquid filament of a complex fluid and to provide guidelines for controlling the droplet formation process.

Direct Inkjet Deposition of Ceramic Green Bodies: II – Jet Behaviour and Deposit Formation

1998 ◽  
Vol 542 ◽  
Author(s):  
N. Reis ◽  
K. A. M. Seerden ◽  
B. Derby ◽  
J. W. Halloran ◽  
J. R. G. Evans
Keyword(s):  
Pulse Shape ◽  
Three Dimensional ◽  
Jet Formation ◽  
Deposit Formation ◽  
On Demand ◽  
Drop On Demand ◽  
Material Systems ◽  
Hot Melt ◽  
Feature Resolution ◽  
Deposition Conditions

AbstractIn order to successfully build three-dimensional shapes by hot-melt inkjet deposition it is essential to control the building block characteristics, i.e., the deposit geometry, dimensions and fine feature resolution. The deposit formation is mainly dependent on the material systems and their jetting behaviour. It is therefore crucial to understand how the jet formation is affected by the inks 'rheological properties and how to manipulate the jet-head driving parameters to achieve optimum deposition conditions. This paper reports our investigations with a model jet firing station, about the influence of driving parameters of hot-melt drop-on-demand print-heads (e.g., pulse shape and frequency) on the jet and deposit formation characteristics, for both unfilled and powder loaded vehicles.

scholarly journals Rheological Investigation of Collagen, Fibrinogen, and Thrombin Solutions for Drop-on-Demand 3D Bioprinting

2020 ◽  
Author(s):  
Hemanth Gudapati ◽  
Daniele Parisi ◽  
Ralph H. Colby ◽  
Ibrahim Ozbolat
Keyword(s):  
Bulk Viscosity ◽  
Flow Behavior ◽  
Three Dimensional ◽  
Protein Solutions ◽  
Ionic Surfactant ◽  
3D Bioprinting ◽  
Rheological Measurements ◽  
On Demand ◽  
Drop On Demand ◽  
Air Interface

<p>Collagen, fibrinogen, and thrombin proteins in aqueous buffer solutions are widely used as precursors of natural biopolymers for three-dimensional (3D) bioprinting applications. The proteins are sourced from animals and their quality may vary from batch to batch, inducing differences in the rheological properties of such solutions. In this work, we investigate the rheological response of collagen, fibrinogen, and thrombin protein solutions in bulk and at the solution/air interface. Interfacial rheological measurements show that fibrous collagen, fibrinogen and globular thrombin proteins adsorb and aggregate at the solution/air interface, forming a viscoelastic solid film at the interface. The viscoelastic film corrupts the bulk rheological measurements in rotational rheometers by contributing to an apparent yield stress, which increases the apparent bulk viscosity up to shear rates as high as 1000 s<sup>-1</sup>. The addition of a non-ionic surfactant, such as polysorbate 80 (PS80) in small amounts between 0.001 and 0.1 v/v%, prevents the formation of the interfacial layer, allowing the estimation of true bulk viscosity and viscoelastic properties of the solutions. The estimation of viscosity not only helps in identifying those protein solutions that are potentially printable with drop-on-demand (DOD) inkjet printing but also detects inconsistencies in flow behavior among the batches.</p>

scholarly journals Rheological investigation of collagen, fibrinogen, and thrombin solutions for drop-on-demand 3D bioprinting

Soft Matter ◽  
2020 ◽  
Vol 16 (46) ◽  
pp. 10506-10517 ◽  
Author(s):  
Hemanth Gudapati ◽  
Daniele Parisi ◽  
Ralph H. Colby ◽  
Ibrahim T. Ozbolat
Keyword(s):  
Three Dimensional ◽  
3D Bioprinting ◽  
Aqueous Buffer ◽  
On Demand ◽  
Buffer Solutions ◽  
Drop On Demand

Collagen, fibrinogen, and thrombin proteins in aqueous buffer solutions are widely used as precursors of natural biopolymers in three-dimensional (3D) bioprinting applications.

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

2015 ◽  
Vol 137 (3) ◽  
Author(s):  
Long Zhao ◽  
Karen Chang Yan ◽  
Rui Yao ◽  
Feng Lin ◽  
Wei Sun
Keyword(s):  
Process Parameters ◽  
Droplet Diameter ◽  
Three Dimensional ◽  
Inertial Force ◽  
Deposition Process ◽  
Droplet Formation ◽  
Solution Viscosity ◽  
Cell Printing ◽  
On Demand ◽  
Drop On Demand

Drop-on-demand (DOD) microdroplet formation and deposition play an important role in additive manufacturing, particularly in printing of three-dimensional (3D) in vitro biological models for pharmacological and pathological studies, for tissue engineering and regenerative medicine applications, and for building of cell-integrated microfluidic devices. In development of a DOD based microdroplet deposition process for 3D cell printing, the droplet formation, controlled on-demand deposition and at the single-cell level, and most importantly, maintaining the viability and functionality of the cells during and after the printing are all remaining to be challenged. This report presents our recent study on developing a novel DOD based microdroplet deposition process for 3D printing by utilization of an alternating viscous and inertial force jetting (AVIFJ) mechanism. The results include an analysis of droplet formation mechanism, the system configuration, and experimental study of the effects of process parameters on microdroplet formation. Sodium alginate solutions are used for microdroplet formation and deposition. Key process parameters include actuation signal waveforms, nozzle dimensional features, and solution viscosity. Sizes of formed microdroplets are examined by measuring the droplet diameter and velocity. Results show that by utilizing a nozzle at a 45 μm diameter, the size of the formed microdroplets is in the range of 52–72 μm in diameter and 0.4–2.0 m/s in jetting speed, respectively. Reproducibility of the system is also examined and the results show that the deviation of the formed microdroplet diameter and the droplet deposition accuracy is within 6% and 6.2 μm range, respectively. Experimental results demonstrate a high controllability and precision for the developed DOD microdroplet deposition system with a potential for precise cell printing.

On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Wonhye Lee ◽  
Vivian Lee ◽  
Samuel Polio ◽  
Phillip Keegan ◽  
Jong-Hwan Lee ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Hydrogel Scaffold ◽  
Freeform Fabrication ◽  
On Demand

Rheological Investigation of Collagen, Fibrinogen, and Thrombin Solutions for Drop-on-Demand 3D Bioprinting

2020 ◽  
Author(s):  
Hemanth Gudapati ◽  
Daniele Parisi ◽  
Ralph H. Colby ◽  
Ibrahim Ozbolat
Keyword(s):  
Bulk Viscosity ◽  
Flow Behavior ◽  
Three Dimensional ◽  
Protein Solutions ◽  
Ionic Surfactant ◽  
3D Bioprinting ◽  
Rheological Measurements ◽  
On Demand ◽  
Drop On Demand ◽  
Air Interface

<p>Collagen, fibrinogen, and thrombin proteins in aqueous buffer solutions are widely used as precursors of natural biopolymers for three-dimensional (3D) bioprinting applications. The proteins are sourced from animals and their quality may vary from batch to batch, inducing differences in the rheological properties of such solutions. In this work, we investigate the rheological response of collagen, fibrinogen, and thrombin protein solutions in bulk and at the solution/air interface. Interfacial rheological measurements show that fibrous collagen, fibrinogen and globular thrombin proteins adsorb and aggregate at the solution/air interface, forming a viscoelastic solid film at the interface. The viscoelastic film corrupts the bulk rheological measurements in rotational rheometers by contributing to an apparent yield stress, which increases the apparent bulk viscosity up to shear rates as high as 1000 s<sup>-1</sup>. The addition of a non-ionic surfactant, such as polysorbate 80 (PS80) in small amounts between 0.001 and 0.1 v/v%, prevents the formation of the interfacial layer, allowing the estimation of true bulk viscosity and viscoelastic properties of the solutions. The estimation of viscosity not only helps in identifying those protein solutions that are potentially printable with drop-on-demand (DOD) inkjet printing but also detects inconsistencies in flow behavior among the batches.</p>

scholarly journals A covalently crosslinked bioink for multi-materials drop-on-demand 3D bioprinting of three-dimensional cell cultures

2021 ◽  
Author(s):  
Robert H. Utama ◽  
Vincent T. G. Tan ◽  
Kristel C. Tjandra ◽  
Andrew Sexton ◽  
Duyen H. T. Nguyen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Error ◽  
Three Dimensional ◽  
Polymer System ◽  
Ductal Adenocarcinoma ◽  
3D Bioprinting ◽  
Cell Models ◽  
On Demand ◽  
Drop On Demand

AbstractIn vitro three-dimensional (3D) cell models have been accepted to better recapitulate aspects of in vivo organ environment than 2D cell culture. Currently, the production of these complex in vitro 3D cell models with multiple cell types and microenvironments remains challenging and prone to human error. Here we report a versatile bioink comprised of a 4-arm PEG based polymer with distal maleimide derivatives as the main ink component and a bis-thiol species as the activator that crosslinks the polymer to form the hydrogel in less than a second. The rapid gelation makes the polymer system compatible with 3D bioprinting. The ink is combined with a drop-on-demand 3D bioprinting platform consisting of eight independently addressable nozzles and high-throughput printing logic for creating complex 3D cell culture models. The combination of multiple nozzles and fast printing logic enables the rapid preparation of many complex 3D structures comprising multiple hydrogel environments in the one structure in a standard 96-well plate format. The platform compatibility for biological applications was validated using pancreatic ductal adenocarcinoma cancer (PDAC) cells with their phenotypic responses controlled by tuning the hydrogel microenvironment.

Study of Structured Porogen Method for Bone Scaffold Fabrication

2008 ◽  
Author(s):  
Lin Lu ◽  
David Wootton ◽  
Peter I. Lelkes ◽  
Jack Zhou
Keyword(s):  
Bone Structure ◽  
Solid Freeform Fabrication ◽  
Three Dimensional ◽  
Bone Scaffold ◽  
Scaffold Fabrication ◽  
Freeform Fabrication ◽  
Bone Scaffolds ◽  
Drop On Demand ◽  
Biomaterial Scaffolds ◽  
Increasing Demand

The increasing demand on bone scaffolds has promoted the development of tissue engineering fabrication technique for manufacturing bone scaffold. In this study, a novel structured porogen method for bone scaffold fabrication has been explored. This method has demonstrated highly efficient and reproducible fabrication of structured bone scaffolds which mimics the bone structure. By using commercially available Drop on Demand (DDP) system and three dimensional printer (3-DP) system, at first designed structured porogens can be manufactured, and then bone scaffolds can be fabricated by injecting the biocomposite materials into the porogens. The mechanical properties of the fabricated scaffolds using DDP system have been characterized. The biocompatibility of our fabricated scaffolds using 3-DP has been examined. With incorporating of bioactive calcium phosphate into the composite materials, the mechanical strength and bioactivity of the scaffolds made by the structured porogen method can be improved significantly. This structured porogen method has a potential to be used on various Solid Freeform Fabrication systems which allows each system to use a single ubiquitous building material to fabricate multiple biomaterial scaffolds with sufficient mechanical integrity.

Fabrication of 3D Temperature Sensor Using Magnetostrictive Inkjet Printhead

2020 ◽  
Vol 64 (5) ◽  
pp. 50405-1-50405-5
Author(s):  
Young-Woo Park ◽  
Myounggyu Noh
Keyword(s):  
Flexible Electronics ◽  
Inkjet Printing ◽  
Temperature Sensor ◽  
Computer Aided Design ◽  
Low Cost ◽  
Three Dimensional ◽  
Drop On Demand ◽  
Aided Design ◽  
Nanoparticle Ink ◽  
Inkjet Printhead

Abstract Recently, the three-dimensional (3D) printing technique has attracted much attention for creating objects of arbitrary shape and manufacturing. For the first time, in this work, we present the fabrication of an inkjet printed low-cost 3D temperature sensor on a 3D-shaped thermoplastic substrate suitable for packaging, flexible electronics, and other printed applications. The design, fabrication, and testing of a 3D printed temperature sensor are presented. The sensor pattern is designed using a computer-aided design program and fabricated by drop-on-demand inkjet printing using a magnetostrictive inkjet printhead at room temperature. The sensor pattern is printed using commercially available conductive silver nanoparticle ink. A moving speed of 90 mm/min is chosen to print the sensor pattern. The inkjet printed temperature sensor is demonstrated, and it is characterized by good electrical properties, exhibiting good sensitivity and linearity. The results indicate that 3D inkjet printing technology may have great potential for applications in sensor fabrication.

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