A Perfused Two-Chamber System for Anticancer Drug Screening

A cell culture microfluidic device has been developed to test the cytotoxicity of anticancer drugs while reproducing multi-organ interactions in vitro. Cells were cultured in separate chambers representing the liver and tumor. The two chambers were connected through a channel to mimick the blood flow. Glioblastoma (GBM) cancer cells (M059K) and hepatoma cells (HepG2) were cultured in the tumor and the liver chambers, respectively. The cytotoxic effect of cancer treatment drug Temolozomide (TMZ) was tested using this two chamber system. The experimental results showed that with the liver cells, the cancer cells showed much higher viability than those without the liver cells. This indicates that the liver metabolism has strong effect on the toxicity of the anticancer drug. The results demonstrated that the perfused two chamber cell culture system has the potential to be used as a platform for drug screening in a more physiologically realistic environment.

Download Full-text

A Cell Culture System That Mimics Chronic Lymphocytic Leukemia Cells Microenvironment for Drug Screening and Characterization

Target Identification and Validation in Drug Discovery - Methods in Molecular Biology ◽

10.1007/978-1-62703-311-4_14 ◽

2013 ◽

pp. 217-226 ◽

Cited By ~ 4

Author(s):

Alessandro Natoni ◽

Michael O’Dwyer ◽

Corrado Santocanale

Keyword(s):

Cell Culture ◽

Chronic Lymphocytic Leukemia ◽

Drug Screening ◽

Culture System ◽

Lymphocytic Leukemia ◽

Leukemia Cells ◽

Cell Culture System ◽

A Cell

Download Full-text

SU-FF-J-104: In Vitro Model to Study the Biological Effect of Dose Gradients On a Cell Culture System

Medical Physics ◽

10.1118/1.1997650 ◽

2005 ◽

Vol 32 (6Part6) ◽

pp. 1944-1944 ◽

Cited By ~ 2

Author(s):

R Bromley ◽

L Oliver ◽

R Harvie ◽

R Davey

Keyword(s):

Cell Culture ◽

Biological Effect ◽

Culture System ◽

In Vitro Model ◽

Cell Culture System ◽

A Cell ◽

Vitro Model

Download Full-text

Three-Dimensional Culture System of Cancer Cells Combined with Biomaterials for Drug Screening

Cancers ◽

10.3390/cancers12102754 ◽

2020 ◽

Vol 12 (10) ◽

pp. 2754 ◽

Cited By ~ 2

Author(s):

Teruki Nii ◽

Kimiko Makino ◽

Yasuhiko Tabata

Keyword(s):

Cell Culture ◽

Cancer Cells ◽

Drug Screening ◽

Culture System ◽

Three Dimensional ◽

3D Culture ◽

New Drugs ◽

Biological Research

Anticancer drug screening is one of the most important research and development processes to develop new drugs for cancer treatment. However, there is a problem resulting in gaps between the in vitro drug screening and preclinical or clinical study. This is mainly because the condition of cancer cell culture is quite different from that in vivo. As a trial to mimic the in vivo cancer environment, there has been some research on a three-dimensional (3D) culture system by making use of biomaterials. The 3D culture technologies enable us to give cancer cells an in vitro environment close to the in vivo condition. Cancer cells modified to replicate the in vivo cancer environment will promote the biological research or drug discovery of cancers. This review introduces the in vitro research of 3D cell culture systems with biomaterials in addition to a brief summary of the cancer environment.

Download Full-text

Characterization of a Cell Culture System of Persistent Hepatitis E Virus Infection in the Human HepaRG Hepatic Cell Line

Viruses ◽

10.3390/v13030406 ◽

2021 ◽

Vol 13 (3) ◽

pp. 406

Author(s):

Marie Pellerin ◽

Edouard Hirchaud ◽

Yannick Blanchard ◽

Nicole Pavio ◽

Virginie Doceul

Keyword(s):

Cell Culture ◽

Culture System ◽

Hepatitis E Virus ◽

Hepatitis E ◽

Inhibitory Effect ◽

Cell Culture System ◽

Efficient Replication ◽

A Cell ◽

Vitro Model

Hepatitis E virus (HEV) is considered as an emerging global health problem. In most cases, hepatitis E is a self-limiting disease and the virus is cleared spontaneously without the need of antiviral therapy. However, immunocompromised individuals can develop chronic infection and liver fibrosis that can progress rapidly to cirrhosis and liver failure. The lack of efficient and relevant cell culture system and animal models has limited our understanding of the biology of HEV and the development of effective drugs for chronic cases. In the present study, we developed a model of persistent HEV infection in human hepatocytes in which HEV replicates efficiently. This HEV cell culture system is based on differentiated HepaRG cells infected with an isolate of HEV-3 derived from a patient suffering from acute hepatitis E. Efficient replication was maintained for several weeks to several months as well as after seven successive passages on HepaRG naïve cells. Moreover, after six passages onto HepaRG, we found that the virus was still infectious after oral inoculation into pigs. We also showed that ribavirin had an inhibitory effect on HEV replication in HepaRG. In conclusion, this system represents a relevant and efficient in vitro model of HEV replication that could be useful to study HEV biology and identify effective antiviral drugs against chronic HEV infection.

Download Full-text

A Multilayered Postconfluent Tumor Cell Culture System for in vitro Drug Screening

Contributions to Oncology - Immunodeficient Animals: Models for Cancer Research ◽

10.1159/000425257 ◽

1996 ◽

pp. 204-208

Author(s):

Evelien Smitskamp-Wilms ◽

Hans R. Hendriks ◽

Paulo E. Pizao ◽

Giuseppe Giaccone ◽

Herbert M. Pinedo ◽

...

Keyword(s):

Cell Culture ◽

Tumor Cell ◽

Drug Screening ◽

Culture System ◽

Cell Culture System ◽

In Vitro Drug Screening ◽

Tumor Cell Culture

Download Full-text

Differences in the Central Energy Metabolism of Cancer Cells between Conventional 2D and Novel 3D Culture Systems

International Journal of Molecular Sciences ◽

10.3390/ijms22041805 ◽

2021 ◽

Vol 22 (4) ◽

pp. 1805

Author(s):

Ryo Ikari ◽

Ken-ichi Mukaisho ◽

Susumu Kageyama ◽

Masayuki Nagasawa ◽

Shigehisa Kubota ◽

...

Keyword(s):

Cell Culture ◽

Cancer Cells ◽

Cancer Cell ◽

Culture System ◽

3D Culture ◽

Cell Culture System ◽

Metabolome Analysis ◽

Wide Range

The conventional two-dimensional (2D) culture is available as an in vitro experimental model. However, the culture system reportedly does not recapitulate the in vivo cancer microenvironment. We recently developed a tissueoid cell culture system using Cellbed, which resembles the loose connective tissue in living organisms. The present study performed 2D and three-dimensional (3D) culture using prostate and bladder cancer cell lines and a comprehensive metabolome analysis. Compared to 3D, the 2D culture had significantly lower levels of most metabolites. The 3D culture system did not impair mitochondrial function in the cancer cells and produce energy through the mitochondria simultaneously with aerobic glycolysis. Conversely, ATP production, biomass (nucleotides, amino acids, lipids and NADPH) synthesis and redox balance maintenance were conducted in 3D culture. In contrast, in 2D culture, biomass production was delayed due to the suppression of metabolic activity. The 3D metabolome analysis using the tissueoid cell culture system capable of in vivo cancer cell culture yielded results consistent with previously reported cancer metabolism theories. This system is expected to be an essential experimental tool in a wide range of cancer research fields, especially in preclinical stages while transitioning from in vitro to in vivo.

Download Full-text

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

Turkish Journal of Biochemistry ◽

10.1515/tjb-2020-0082 ◽

2020 ◽

Vol 45 (5) ◽

pp. 631-637

Author(s):

Cansu Ozel-Tasci ◽

Gozde Pilatin ◽

Ozgur Edeer ◽

Sukru Gulec

Keyword(s):

Cell Culture ◽

Culture System ◽

Metabolic Diseases ◽

Enzymatic Digestion ◽

Cell Culture System ◽

Culture Studies ◽

Experimental Approaches ◽

In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

Download Full-text