Serological Cross-reaction among Bartonella henselae, Chlamydia pneumoniae and Coxiella burnetii by Indirect Fluorescence Antibody Method

Cats naturally exposed to Ehrlichia canis have been described in different regions of the world, but little is known about the genotypes associated with infection in these animals. To detect E. canis-specific antibodies and investigate the E. canis TRP genotypes in cats, serum samples from 76 domestic cats reactive to crude E. canis antigens by the indirect fluorescence antibody test (IFAT) were analyzed by ELISA, using E. canis-specific peptides (i.e., TRP19 and TRP36 /BR/US/CR). Of these, 25 (32.9%) cats reacted to at least one TRP peptide, confirming their specific exposure to E. canis. Eighteen (23.7%) cats reacted to TRP19, 15 (19.8%) to BRTRP36, and 11 (14.5%) to USTRP36, but none of them reacted to CRTRP36. Eight (10.5%) cats reacted to TRP19 but not to any TRP36 genotype, demonstrating the possible existence of a new E. canis genotype infecting felines. Nevertheless, this study provides the first report of anti-E. canis-specific antibodies in domestic cats.

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Inhibition of Fibroblast Apoptosis by Borrrelia afzelii, Coxiella burnetii and Bartonella henselae

Polish Journal of Microbiology ◽

10.33073/pjm-2011-038 ◽

2011 ◽

Vol 60 (3) ◽

pp. 269-272 ◽

Cited By ~ 3

Author(s):

TOMASZ CHMIELEWSKI ◽

STANISŁAWA TYLEWSKA-WIERZBANOWSKA

Keyword(s):

Cell Death ◽

Chronic Disease ◽

Coxiella Burnetii ◽

Caspase 3 ◽

Bartonella Henselae ◽

Tissue Homeostasis ◽

Host Cells ◽

Fibroblast Cells ◽

Human Fibroblast Cells ◽

Long Time

Apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis. The aim of this study was to investigate the influence of Borrelia afzelii, Coxiella burnetii, and Bartonella henselae bacteria on apoptosis measured as the level of caspase 3 activity in human fibroblast cells HEL-299. Our findings show that C. burnetii bacteria may inhibit the process of apoptosis in the host cells for a long time. This can permit intracellular survival in the host and mediatingthe development of chronic disease.

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The Bartonella henselae sucB gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease

Journal of Medical Microbiology ◽

10.1099/jmm.0.45616-0 ◽

2004 ◽

Vol 53 (12) ◽

pp. 1221-1227 ◽

Cited By ~ 8

Author(s):

Christine M Litwin ◽

Joel M Johnson ◽

Thomas B Martins

Keyword(s):

Coxiella Burnetii ◽

Brucella Melitensis ◽

Bartonella Henselae ◽

Immunoblot Analysis ◽

Cross Reactivity ◽

Cosmid Library ◽

Cat Scratch Disease ◽

Igg Antibodies ◽

Bacillary Angiomatosis ◽

Dihydrolipoamide Succinyltransferase

Bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. A recombinant clone expressing an immunoreactive antigen of B. henselae was isolated by screening a genomic DNA cosmid library by Western blotting with sera pooled from patients positive for B. henselae IgG antibodies by indirect immunofluorescence (IFA). The deduced amino acid sequence of the 43.7 kDa encoded protein was found to be 76.3 % identical to the dihydrolipoamide succinyltransferase enzyme (SucB) of Brucella melitensis. SucB has been shown to be an immunogenic protein during infections by Brucella melitensis, Coxiella burnetii and Bartonella vinsonii. The agreement between reactivity with a recombinant SucB fusion protein on immunoblot analysis and the results obtained by IFA was 55 % for IFA-positive sera and 88 % for IFA-negative sera. Cross-reactivity was observed with sera from patients with antibodies against Brucella melitensis, Mycoplasma pneumoniae, Francisella tularensis, Coxiella burnetii and Rickettsia typhi.

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Detection of potentially pathogenic bacteria from Ixodes ricinus carried by pets in Tuscany, Italy

Veterinary Record Open ◽

10.1136/vetreco-2020-000395 ◽

2020 ◽

Vol 7 (1) ◽

pp. e000395

Author(s):

Valentina Chisu ◽

Cipriano Foxi ◽

Gabriella Masu ◽

Barbara D' Amaddio ◽

Giovanna Masala

Keyword(s):

Coxiella Burnetii ◽

Pathogenic Bacteria ◽

Bartonella Henselae ◽

Companion Animals ◽

Chlamydia Psittaci ◽

Sequencing Analysis ◽

Increased Risk ◽

Domestic Environments ◽

Preliminary Study ◽

Potential Public Health

BackgroundTicks are vectors of disease-causing pathogens that pose a serious threat to animals and people. Dogs and cats are exposed to tick infestation in multiple ways and can easily transport infected ticks into domestic environments and potentially transfer them to people. Pet owners are at increased risk of picking up ticks from their pets and developing tickborne diseases. This study aims to detect the presence of pathogens of potential public health interest in ticks removed from cats and dogs in Tuscany, Italy.MethodsThe collected ticks were screened for the presence of protozoan (Theileria species and Babesia species) and bacterial (Rickettsia species, Anaplasma species, Ehrlichia species, Chlamydia species, Bartonella species and Coxiella burnetii) pathogens using PCR.ResultsPCR and sequencing analysis revealed that 3 per cent of the ticks were PCR-positive for the presence of Rickettsia helvetica DNA, 5 per cent of ticks were PCR-positive for Bartonella henselae DNA, and 46 per cent of ticks were PCR-positive for Chlamydia psittaci and Chlamydia abortus DNA. None of the examined ticks was PCR-positive for Theileria species, Babesia species, Anaplasma species, Ehrlichia canis or Coxiella burnetii DNA.ConclusionThe results of this preliminary study highlight the importance of monitoring companion animals as indicators to evaluate the health status of their owners. Preventive measures are necessary to limit the spread of zoonotic pathogens from companion animals to people within the home environment.

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Exposure and Risk Factors to Coxiella burnetii, Spotted Fever Group and Typhus Group Rickettsiae, and Bartonella henselae among Volunteer Blood Donors in Namibia

PLoS ONE ◽

10.1371/journal.pone.0108674 ◽

2014 ◽

Vol 9 (9) ◽

pp. e108674 ◽

Cited By ~ 10

Author(s):

Bruce H. Noden ◽

Filippus I. Tshavuka ◽

Berta E. van der Colf ◽

Israel Chipare ◽

Rob Wilkinson

Keyword(s):

Risk Factors ◽

Coxiella Burnetii ◽

Blood Donors ◽

Bartonella Henselae ◽

Spotted Fever ◽

Spotted Fever Group

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Seroprevalence of Antibodies to Microorganisms Known To Cause Arterial and Myocardial Damage in Patients with or without Coronary Stenosis

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.8.5.997-1002.2001 ◽

2001 ◽

Vol 8 (5) ◽

pp. 997-1002 ◽

Cited By ~ 11

Author(s):

C. Stöllberger ◽

G. Mölzer ◽

J. Finsterer

Keyword(s):

Coronary Artery ◽

Chlamydia Pneumoniae ◽

Bartonella Henselae ◽

Treponema Pallidum ◽

Myocardial Damage ◽

Percutaneous Transluminal Coronary Angioplasty ◽

Rickettsia Conorii ◽

Exertional Dyspnea ◽

H Pylori ◽

Transluminal Coronary Angioplasty

ABSTRACT Infections are assumed to play a role in coronary artery disease (CAD) and cardiomyopathies. It is unknown whether the seroprevalence of antibodies to these microorganisms is higher in patients with than without CAD. The seroprevalence of antibodies to Bartonella henselae, Borrelia burgdorferi, Chlamydia pneumoniae, Coxiella burnetii, Helicobacter pylori, human granulocyticEhrlichia, Leptospira, Rickettsia conorii, andTreponema pallidum was assessed prospectively in patients with exertional dyspnea or anginal chest pain who underwent coronary angiography because of suspected CAD. Patients with normal angiograms (NA) were those in whom no more than 50% stenosis of any coronary artery was found. Patients with CAD were patients who underwent percutaneous transluminal coronary angioplasty. There were 50 patients with CAD (9 female) and 62 with NA (25 female), with a mean age of 62 years. All patients had antibodies to at least one microorganism: to B. henselae, 8% of CAD patients and 5% of NA patients; to B. burgdorferi IgG, 14% CAD and 6% NA; to B. burgdorferi IgM, 6% CAD and 3% NA; to C. pneumoniae lipopolysaccharide (LPS) IgA, 76% CAD and 77% NA; to C. pneumoniae LPS IgG, 80% CAD and 90% NA; to C. burnetii, 0% CAD and 5% NA; to H. pylori, 92% CAD and 68% NA; to human granulocytic Ehrlichia, 8% CAD and 3% NA; toLeptospira IgG, 4% CAD and 2% NA; to R. conorii, 10% in both groups; and to T. pallidum, 2% CAD and 0% NA. The seroprevalence of antibodies to micro-organisms known to induce arterial and myocardial damage does not differ between patients with CAD and NA.

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