First report of Varroa genotype in western Asia based on genotype identification of Iranian Varroa destructor populations (Mesostigmata: Varroidae) using RAPD marker

2018 ◽  
Vol 23 (2) ◽  
pp. 199 ◽  
Author(s):  
Zahra Hajializadeh ◽  
Mahdieh Asadi ◽  
Hamidreza Kavousi
Keyword(s):  
Genetic Structure ◽  
Dna Sequence ◽  
Varroa Destructor ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Molecular Genetic ◽  
Molecular Genetic Study ◽  
Western Asia ◽  
Genotype Identification ◽  
Southern Half

Random amplified polymorphic DNA (RAPD) technique was used to the analysis of the haplotypes of Varroa destrcuctor in southern half of Iran. Eight Varroa populations were collected and five RAPD primers were used to determine their genotype. All samples showed the K pattern genotype. Furthermore 30 loci were amplified; from which 29 were polymorphic (96.67%) and 1 was monomorphic (3.33%). This is the first molecular genetic study of V. destructor in Iran. Detailed investigations at DNA sequence level are needed to characterize genetic structure of V. destructor populations in Iran.

scholarly journals 462 PB 295 RAPD MAPPING OF THE ASPARAGUS (ASPARAGUS OFFICINALIS L.) GENOME

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 497d-497
Author(s):  
Chunxiao Jiang ◽  
Kenneth C. Sink
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Molecular Genetic ◽  
Female Parent ◽  
Male Parent ◽  
Asparagus Officinalis ◽  
Linkage Groups ◽  
Sex Type ◽  
Group Sex ◽  
Sex Locus

RAPD (Random Amplified Polymorphic DNA) is being utilized to construct a molecular genetic linkage map. The mapping population used is comprised of 65 individuals. Of the 240 decamer primers (Operon) tested to date, 50 polymorphic bands have been resolved. The 50 markers are divided into two groups according to the genotypes of the two parents. The first group is where the female parent is heterozygous and male parent is homozygous and it has 17 markers, while the second group, in which male parent is heterozygous and female parent is homozygous, has 33 markers. Analysis of the two groups by MAPMAKER indicated that two linkage groups are formed in the first group, and five linkage groups are formed in the second group. Sex type is controlled by the M locus. Genetic experiments have demonstrated that females are homogametic (mm) while male plants are heterogametic (Mm) for the sex locus. Two bulks of genomic DNA created by using 10 male and 10 female individuals in the population were screened to identify RAPD markers associated with the sex locus. RAPD marker OCP15-984 is closely linked with the M locus (7.6 cM).

scholarly journals The genetic structure of the herd according to the GDF9, GH genes in Volgograd and Edilbaevsky sheep breeds

2021 ◽  
Vol 14 ◽  
pp. 51-59
Author(s):  
I.F. Gorlov ◽  
◽  
M.I. Slozhenkina ◽  
Y.A. Kolosov ◽  
N.V. Shirokova ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Rural Areas ◽  
Molecular Genetic ◽  
Cartilage Tissue ◽  
Resource Saving ◽  
Molecular Genetic Study ◽  
Differential Growth ◽  
Genetic Studies ◽  
Study Results ◽  
Selection Of

Aim. The study of the genetic structure of sheep of the Volgograd and Edilbaevsky breeds according to the GDF9, GH genes. Materials and Methods. The research was carried out in herds of sheep from leading breeding plants, on the basis of Don State Agrarian University and Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production. Biosamples of skin and cartilage tissue from the auricles were taken from sheep by plucking an area of 1 cm² for molecular genetic studies. Evaluation was performed by PCR-RFLP method. Endonucleases BstHH HaeIII were used to restrict the amplified regions of the GDF9 and GH genes. The presence and frequency of alleles and genotypes for the GDF9, GH genes were determined based on the results of a molecular genetic study. Results. The article is devoted to the study of the genetic structure of sheep of the Volgograd and Edilbaevsky breeds. One of the important resources for creating highly productive animals is the use of marker selection as an additional criterion for the selection and selection of breeding animals. The use of DNA markers for the selection of animals containing desirable alleles of genes of economically valuable traits is one of the new scientific approaches to solve this problem. The development of sheep breeding in our country will allow using the available natural and human resources in rural areas, as well as obtaining high-quality lamb through the use of resource-saving technologies. The aim of the work was to study the genetic structure of sheep of the Volgograd and Edilbaevsky breeds according to the GDF9, GH genes. To perform molecular genetic studies, samples of ear plucks (tissue from the auricle) with an area of 1 cm2 were taken from sheep. The assessment was carried out by PCR-PDRF. In all the studied groups of sheep, A and B allelic variants of the GDF9 gene, alleles A and B of the GH gene were established. The analysis of the data revealed a breed aspect in the distribution of the frequency of alleles and genotypes of the GDF9, GH genes in the populations under consideration. Conclusion. The results of the study of the genetic structure of the populations of sheep of the Volgograd and Edilbaevsky breeds showed that the frequency distribution of alleles and genotypes of the growth hormone (GH) and differential growth factor (GDF9) genes have some features associated with the breed affiliation of the studied sheep.

Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

2013 ◽  
Vol 20 (1-2) ◽  
pp. 1-8
Author(s):  
MM Rahman ◽  
L Rahman ◽  
SN Begum ◽  
F Nur
Keyword(s):  
Genetic Distance ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Polymorphic Loci ◽  
Rice Genotypes ◽  
High Level ◽  
Genetic Diversity Study ◽  
Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Nei’s genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 – 8, 2009

Challenges in the differential diagnosis of multiple endocrine neoplasia syndrome type 1 with isolated family hyperparathyroidism

2020 ◽  
Vol 98 (3) ◽  
pp. 218-225
Author(s):  
J. A. Krupinova ◽  
N. G. Mokrysheva ◽  
N. Y. Kalinchenko ◽  
A. K. Eremkina ◽  
A. N. Polyakov ◽  
...  
Keyword(s):  
Multiple Endocrine Neoplasia ◽  
Molecular Genetic ◽  
Pancreatic Neuroendocrine Tumor ◽  
Family Type ◽  
Dynamic Monitoring ◽  
Heterozygous Mutation ◽  
Molecular Genetic Study ◽  
Men 1 ◽  
Endocrine Neoplasia

Multiple endocrine neoplasia type 1 (MEN-1) is the most common cause of the hereditary type of primary hyperparathyroidism (PHPT). If a family type of PHPT is suspected, a dynamic monitoring of patients and their close relatives should be carried out throughout their lives. We present a clinical case of a family in which four members of a pedigree were diagnosed with familial isolated hyperparathyroidism (FIHP). The diagnosis was changed to MEN-1, because it appeared that one of the patients had pancreatic neuroendocrine tumor. Molecular genetic study of MEN1 by direct by means of Sanger sequencing revealed that six family members had a new heterozygous mutation in exon 9: s. 1252 G> T p. D418Y.

scholarly journals Population Genetic Structure Analysis Reveals Decreased but Moderate Diversity for the Oriental Fire-Bellied Toad Introduced to Beijing after 90 Years of Independent Evolution

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1429
Author(s):  
Yang Teng ◽  
Jing Yang ◽  
Guofen Zhu ◽  
Fuli Gao ◽  
Yingying Han ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Structure Analysis ◽  
Phylogenetic Trees ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Haplotype Diversity ◽  
Botanical Garden ◽  
Source Population ◽  
Independent Evolution ◽  
Loop Region

Detailed molecular genetic research on amphibian populations has a significant role in understanding the genetic adaptability to local environments. The oriental fire-bellied toads (Bombina orientalis) were artificially introduced to Beijing from Shandong Province in 1927, and since then, this separated population went through an independent evolution. To explore the differentiation of the introduced population with its original population, this study analyzed the genetic structure of the oriental fire-bellied toad, based on the mitochondrial genome control region and six microsatellite sites. The results showed that the haplotype diversity and nucleotide diversity of the mitochondrial D-loop region partial sequences of the Beijing Botanical Garden population and the Baiwangshan population were lower than those of the Shangdong Kunyushan population. Microsatellite marker analysis also showed that the observed heterozygosity and expected heterozygosity of the Beijing populations were lower than those of the Kunyushan population. The phylogenetic trees and network diagrams of haplotypes indicated that the three populations were not genetically separated. However, the structure analysis showed a genetic differentiation and categorized the sampling individuals into Beijing and Shandong genetic clusters, which indicated a tendency for isolated evolution in the Beijing population. Although the Beijing populations showed a decline in genetic diversity, it was still at a moderate level, which could maintain the survival of the population. Thus, there is no need to reintroduce new individuals from the Kunyushan source population.

Dedifferentiated endometrial carcinomas with neuroendocrine features: a clinicopathologic, immunohistochemical, and molecular genetic study

2018 ◽  
Vol 72 ◽  
pp. 100-106 ◽  
Author(s):  
Iñigo Espinosa ◽  
Antonio De Leo ◽  
Emanuela D'Angelo ◽  
Juan M. Rosa-Rosa ◽  
Marina Corominas ◽  
...  
Keyword(s):  
Genetic Study ◽  
Molecular Genetic ◽  
Molecular Genetic Study ◽  
Endometrial Carcinomas
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