A new registration evaluation index of multi-mode images

Author(s):  
Yingjie Li ◽  
Eryou Ji ◽  
Ning Li ◽  
Guosheng Chen ◽  
Lingzhi Xing
Keyword(s):  
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.


TAPPI Journal ◽  
2010 ◽  
Vol 9 (6) ◽  
pp. 34-39
Author(s):  
AIYU QU ◽  
YANHUI AO ◽  
JUN YAN ◽  
GUIGAN FANG

To develop new wood cellulose resources and fast-growing pulpwood plantation fiber sources, it is very important to evaluate their pulping properties. A comprehensive multi-index pulping-suitability evaluation model is investigated in this paper by considering four fast-growing wood species. First, a new evaluation-index system for kraft pulp was developed based on traditional evaluation-index systems. Then, the membership degree of every index was analyzed to obtain a fuzzy matrix. The proportional contribution of each parameter to the main pulping properties could then be determined. Finally, a comprehensive evaluation model of kraft pulp properties was developed. The model is reliable compared with traditional assessment methods. The results confirmed the feasibility and rationality of developing new wood cellulose resources and fast-growing pulpwood plantations using fuzzy comprehensive evaluations.


2009 ◽  
Vol E92-B (12) ◽  
pp. 3717-3725
Author(s):  
Thomas HUNZIKER ◽  
Ziyang JU ◽  
Dirk DAHLHAUS

2014 ◽  
Vol E97.C (7) ◽  
pp. 781-786 ◽  
Author(s):  
Mohammad NASIR UDDIN ◽  
Takaaki KIZU ◽  
Yasuhiro HINOKUMA ◽  
Kazuhiro TANABE ◽  
Akio TAJIMA ◽  
...  

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