High speed large-field-of-view scanning microscopy imaging technology and system implementation

Author(s):  
Liang Shan ◽  
Heng Mao ◽  
Muyue Zhai ◽  
Shuxiang Dong ◽  
Louis Tao
Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 996 ◽  
Author(s):  
Ran Chen ◽  
Zhongwei Li ◽  
Kai Zhong ◽  
Xingjian Liu ◽  
Yonghui Wu ◽  
...  

The ram speed of a steam hammer is an important parameter that directly affects the forming performance of forgers. This parameter must be monitored regularly in practical applications in industry. Because of the complex and dangerous industrial environment of forging equipment, non-contact measurement methods, such as stereo vision, might be optimal. However, in actual application, the field of view (FOV) required to measure the steam hammer is extremely large, with a value of 2–3 m, and heavy steam hammer, at high-speed, usually causes a strong vibration. These two factors combine to sacrifice the accuracy of measurements, and can even cause the failure of measurements. To solve these issues, a bundle-adjustment-principle-based system calibration method is proposed to realize high-accuracy calibration for a large FOV, which can obtain accurate calibration results when the calibration target is not precisely manufactured. To decrease the influence of strong vibration, a stationary world coordinate system was built, and the external parameters were recalibrated during the entire measurement process. The accuracy and effectiveness of the proposed technique were verified by an experiment to measure the ram speed of a counterblow steam hammer in a die forging device.


Author(s):  
Che-Hang Yu ◽  
Jeffrey N. Stirman ◽  
Yiyi Yu ◽  
Riichiro Hira ◽  
Spencer L. Smith

AbstractImaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large Field-of-view Two-Photon imaging (Diesel2p). Combining novel optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view (∼ 25 mm2) with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.


2019 ◽  
Vol 25 (S2) ◽  
pp. 46-47
Author(s):  
Yi Jiang ◽  
Junjing Deng ◽  
Jeffrey A. Klug ◽  
Yudong Yao ◽  
Curt Preissner ◽  
...  
Keyword(s):  

Author(s):  
Yongxiao Li ◽  
Yean J. Lim ◽  
Qiongkai Xu ◽  
Lynette Beattie ◽  
Elizabeth E. Gardiner ◽  
...  

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shaun Pacheco ◽  
Chengliang Wang ◽  
Monica K. Chawla ◽  
Minhkhoi Nguyen ◽  
Brend K. Baggett ◽  
...  

Author(s):  
Kenneth F. Maassen ◽  
Farzad Poursadegh ◽  
Caroline L. Genzale

Abstract Modern high-efficiency engines utilize direct injection for charge preparation at extremely high pressures. At these conditions the scales of atomization become challenging to measure, as primary breakup occurs on the micrometer and nanosecond scales. As such, fuel sprays at these conditions have proven difficult to study via direct imaging. While high-speed cameras now exist that can shutter at tens to hundreds of nanoseconds, and long-range microscopes can be coupled to these cameras to provide high resolution images, the resolving power of these systems is typically limited by pixel size and field of view. The large pixel sizes make the realization of the diffraction-limited optical resolution quite challenging. On the other hand, limited data throughput under high repetition rate operation limits the field of view (FOV) due to reduced sensor area. Therefore, a novel measurement technique is critical to study fuel spray formation at engine-relevant conditions. In this work, we demonstrate a new high-resolution imaging technique, Spectral Microscopy, which aims to realize diffraction-limited imaging at effective framerates sufficient for capturing primary breakup in engine-relevant sprays. A spectral microscopy system utilizing a consumer-grade DSLR allows for significantly wider FOV with improved resolving power compared to high-speed cameras. Temporal shuttering is accomplished via separate and independently triggered back illumination sources, with wavelengths selected to overlap with the detection bands of the camera sensor’s RGB filter array. The RGB detection channels act as filters to capture independently timed red, green, and blue light pulses, enabling the capture of a three consecutive images at effective framerates exceeding 20 Million fps. To optimize system performance, a backlit illumination system is designed to maximize light throughput, a multi-lens setup is created, and an image processing algorithm is demonstrated that formulates a three-frame image from the camera sensor. The system capabilities are then demonstrated by imaging engine relevant diesel sprays. The spectral microscopy system detailed in this paper allows for micron-scale feature recognition at framerates exceeding 20 Million fps, thus expanding the capability for experimental research on primary breakup in fuel sprays for modern direct-injection engines.


2013 ◽  
Vol 380-384 ◽  
pp. 3758-3761 ◽  
Author(s):  
Long Xu Jin ◽  
Jin Li ◽  
Xian Peng Hao

In order to satisfy the transmission of mass data volume of on-board time delay integration charge coupled devices (TDICCD) image, a high speed image transmission system of space panchromatic TDICCD camera with a large field of view is designed in this paper. A 4-channel parallel high speed transmission system for 16-channel CCD image was designed. A high speed serializer TLK2711 is applied to send and receive multi channel TDICCD images. A single chip TLK2711 can processing 4 channels image. Experimental results show that the designed transmission system can reliably work. The designed transmission system can meet the application for space TDICCD camera with a large field of view.


Sign in / Sign up

Export Citation Format

Share Document