A Simple Way to Demonstrate Fluorescent Image by Employing a Conventional Microscope and Transparent Colored Acrylic Pieces

2021 ◽  
Vol 59 (1) ◽  
pp. 57-59
Author(s):  
Ngo Khoa Quang
Keyword(s):  
Fluorescent Image ◽  
Conventional Microscope

Closing the GAP—A 5Å Scanning Microscope

1969 ◽  
Vol 27 ◽  
pp. 6-7
Author(s):  
A. V. Crewe
Keyword(s):  
Normal Form ◽  
The Other ◽  
Resolving Power ◽  
Scanning Microscope ◽  
Conventional Microscope ◽  
Other Hand ◽  
Closing The Gap ◽  
Thermal Sources ◽  
Better Than ◽  
Transmission Microscope

We have become accustomed to differentiating between the scanning microscope and the conventional transmission microscope according to the resolving power which the two instruments offer. The conventional microscope is capable of a point resolution of a few angstroms and line resolutions of periodic objects of about 1Å. On the other hand, the scanning microscope, in its normal form, is not ordinarily capable of a point resolution better than 100Å. Upon examining reasons for the 100Å limitation, it becomes clear that this is based more on tradition than reason, and in particular, it is a condition imposed upon the microscope by adherence to thermal sources of electrons.

The Potentials of Scanning Microscopy

1968 ◽  
Vol 26 ◽  
pp. 352-353
Author(s):  
A. V. Crewe
Keyword(s):  
Salient Feature ◽  
Secondary Emission ◽  
Resolving Power ◽  
X Rays ◽  
Scanning Microscope ◽  
Forming Process ◽  
Additional Tool ◽  
Detection Systems ◽  
Conventional Microscope ◽  
Present Information

If the resolving power of a scanning electron microscope can be improved until it is comparable to that of a conventional microscope, it would serve as a valuable additional tool in many investigations.The salient feature of scanning microscopes is that the image-forming process takes place before the electrons strike the specimen. This means that several different detection systems can be employed in order to present information about the specimen. In our own particular work we have concentrated on the use of energy loss information in the beam which is transmitted through the specimen, but there are also numerous other possibilities (such as secondary emission, generation of X-rays, and cathode luminescence).Another difference between the pictures one would obtain from the scanning microscope and those obtained from a conventional microscope is that the diffraction phenomena are totally different. The only diffraction phenomena which would be seen in the scanning microscope are those which exist in the beam itself, and not those produced by the specimen.

scholarly journals Clinical implementation of a 3D4K-exoscope (Orbeye) in microneurosurgery

2021 ◽  
Author(s):  
Judith Rösler ◽  
Stefan Georgiev ◽  
Anna L. Roethe ◽  
Denny Chakkalakal ◽  
Güliz Acker ◽  
...  
Keyword(s):  
User Satisfaction ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Intraoperative Complications ◽  
Rank Test ◽  
User Group ◽  
Clinical Implementation ◽  
Physical Strain ◽  
Conventional Microscope ◽  
The Impact

AbstractExoscopic surgery promises alleviation of physical strain, improved intraoperative visualization and facilitation of the clinical workflow. In this prospective observational study, we investigate the clinical usability of a novel 3D4K-exoscope in routine neurosurgical interventions. Questionnaires on the use of the exoscope were carried out. Exemplary cases were additionally video-documented. All participating neurosurgeons (n = 10) received initial device training. Changing to a conventional microscope was possible at all times. A linear mixed model was used to analyse the impact of time on the switchover rate. For further analysis, we dichotomized the surgeons in a frequent (n = 1) and an infrequent (n = 9) user group. A one-sample Wilcoxon signed rank test was used to evaluate, if the number of surgeries differed between the two groups. Thirty-nine operations were included. No intraoperative complications occurred. In 69.2% of the procedures, the surgeon switched to the conventional microscope. While during the first half of the study the conversion rate was 90%, it decreased to 52.6% in the second half (p = 0.003). The number of interventions between the frequent and the infrequent user group differed significantly (p = 0.007). Main reasons for switching to ocular-based surgery were impaired hand–eye coordination and poor depth perception. The exoscope investigated in this study can be easily integrated in established neurosurgical workflows. Surgical ergonomics improved compared to standard microsurgical setups. Excellent image quality and precise control of the camera added to overall user satisfaction. For experienced surgeons, the incentive to switch from ocular-based to exoscopic surgery greatly varies.

scholarly journals A deep generative model of 3D single-cell organization

2021 ◽  
Author(s):  
Rory Donovan-Maiye ◽  
Jackson Brown ◽  
Caleb Chan ◽  
Liya Ding ◽  
Calysta Yan ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Morphology ◽  
Image Data ◽  
Subcellular Structure ◽  
Cell Image ◽  
Fluorescent Image ◽  
Trade Offs ◽  
Cell Organization ◽  
Latent Representations ◽  
Target Effects

We introduce a framework for end-to-end integrative modeling of 3D single-cell multi-channel fluorescent image data of diverse subcellular structures. We employ stacked conditional β-variational autoencoders to first learn a latent representation of cell morphology, and then learn a latent representation of subcellular structure localization which is conditioned on the learned cell morphology. Our model is flexible and can be trained on images of arbitrary subcellular structures and at varying degrees of sparsity and reconstruction fidelity. We train our full model on 3D cell image data and explore design trade-offs in the 2D setting. Once trained, our model can be used to impute structures in cells where they were not imaged and to quantify the variation in the location of all subcellular structures by generating plausible instantiations of each structure in arbitrary cell geometries. We apply our trained model to a small drug perturbation screen to demonstrate its applicability to new data. We show how the latent representations of drugged cells differ from unperturbed cells as expected by on-target effects of the drugs.

scholarly journals Mathematical Aspects of Using Electrostatic Filters in Food Industry

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Mircea Valentin MUNTEAN ◽  
Ioan DROCAS ◽  
Ovidiu MARIAN ◽  
V BARBIERU ◽  
C. TOPAN
Keyword(s):  
Cost Effectiveness ◽  
Food Industry ◽  
Naked Eye ◽  
Conventional Microscope ◽  
Air Suspension ◽  
Fluid Particles

Purifying processes on gas or liquid streams are often required to treat such large volumes of fluid. Particles in air suspension will range downwards from 100 m. Down to 20 m they will be visible to naked eye, while on down to 0,1...0,2 m they can be observed with a conventional microscope. Electrostatic filters are used in food industry to purify pollute emission and climate the precincts. Characteristic of electrostatic separate operation is: efficiency, level of filtration needed, dust storage capability, cost effectiveness, temperature of suspension, velocities in still air.

scholarly journals The Use And Benefits of 3D Digital Microscope In Hepatic Artery Reconstruction During Living Donor Liver Transplant

2021 ◽  
Author(s):  
Ching-Min Lin ◽  
Shih-Lung Lin ◽  
Yu-Ju Hung ◽  
Chia-En Hsieh ◽  
Yao-Li Chen ◽  
...  
Keyword(s):  
Liver Transplant ◽  
Hepatic Artery ◽  
Surgical Training ◽  
Living Donor ◽  
Donor Liver ◽  
Conventional Microscope ◽  
Living Donor Liver Transplant ◽  
Digital Microscope ◽  
Donor Liver Transplant ◽  
Artery Reconstruction

Abstract Background: To compare the intraoperative process of hepatic artery anastomosis using conventional microscope and novel 3D digital microscope and to share our institute’s experience for better surgical training.Method: A retrospective cohort study with 46 hepatic artery reconstructions in living donor liver transplant patients via operational microscope (Control group) and 3D digital microscope Mitaka Kestrel View II (Study group). We then discuss and share our institution’s experience to improve surgical training. Results: Both operation instruments provide effective and comparable results. There was no statistical significance regarding operational objective results between conventional microscope and exoscope. Both instruments have no hepatic artery size limit, and both have complete vessel patency rate. Conclusions: There was no statistical differences regarding hepatic artery anastomosis between microscope and exoscope cohorts. However, exoscope provided better ergonomics in the operation room and lessen musculoskeletal strain, allowing surgeons to work in a more neutral and comfortable posture and allow first assistant to learn and assist more effectively. Using exoscope with micro-forceps and modified tie technique make artery reconstruction easier.

Cognitive workload, complications and visual outcomes of phacoemulsification cataract surgery: Three-dimensional versus conventional microscope

2021 ◽  
pp. 112067212110620
Author(s):  
Aditya Kelkar ◽  
Jai Kelkar ◽  
Yogesh Chougule ◽  
Mounika Bolisetty ◽  
Priyanka Singhvi
Keyword(s):  
Cataract Surgery ◽  
Three Dimensional ◽  
Posterior Chamber ◽  
Dimensional System ◽  
Cognitive Workload ◽  
Visualization System ◽  
Visual Outcomes ◽  
Conventional Microscope ◽  
Three Dimensional System ◽  
Phacoemulsification Cataract Surgery

Purpose To compare the surgical workload, complications, and visual outcomes using the three-dimensional visualization system with the conventional microscope in phacoemulsification cataract surgery. Design Prospective, non-randomized, open-label interventional study. Methods All patients underwent phacoemulsification cataract surgery using the three-dimensional visualization system or conventional microscope. Results Of the 203 eyes, 80 underwent surgery with the three-dimensional system while 123 underwent with the conventional microscope. No difference was noted in the total surgical duration, complication rates, and visual outcomes between the two groups. However, capsulorhexis was significantly faster using the conventional microscope while posterior chamber intraocular lens insertion was quicker using the three-dimensional system. In terms of cognitive workload comparison, no difference was seen in the surgeons’ heart rate, oxygen saturation levels, and surgery task load index total workload score and workload score for all six dimensions of the questionnaire, between the three-dimensional system and conventional microscope groups. As compared to baseline, the heart rate increased significantly during all surgical steps and at the end in both groups. When compared to baseline, the oxygen saturation levels were significantly raised during capsulorhexis, irrigation, and aspiration and posterior chamber intraocular lens insertion and at the end of the surgery in the three-dimensional group and during incision and at the end of the surgery in the conventional microscope group. Conclusions The duration of surgery, complications, and visual acuity outcomes remain unaffected while performing phacoemulsification cataract surgeries with the three-dimensional viewing system when compared to the conventional microscopes. Moreover, the surgeons’ cognitive workload too remains unaffected while utilizing this revolutionary three-dimensional surgical technology.

Multiple Iteractive Labeling by Antibody Neodeposition (MILAN)

2019 ◽  
Author(s):  
Giorgio Cattoretti ◽  
Francesca Maria Bosisio ◽  
Lukas Marcelis ◽  
Maddalena Maria Bolognesi
Keyword(s):  
Image Analysis ◽  
Tissue Section ◽  
Indirect Immunofluorescence ◽  
Analysis Software ◽  
Tissue Sections ◽  
Image Analysis Software ◽  
Single Section ◽  
Fluorescent Image ◽  
Secondary Antibodies

Abstract Multiplexing, labeling for multiple immunostains the very same cell or tissue section in situ, is of considerable interest. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, scarcity of specialized skills or facilities. We have validated and detail here a method based on common primary and secondary antibodies, diffusely available fluorescent image scanners and routinely processed tissue sections \(FFPE). It entails rounds of four-color indirect immunofluorescence, image acquisition and removal \(stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulphide cleavage. In excess of 50 different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software.

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