Application of Directly Coupled High Performance Liquid Chromatography-NMR-Mass Spectometry and 1H NMR Spectroscopic Studies to the Investigation of 2,3-Benzofuran Metabolism in  Sprague-Dawley Rats

The purpose of this study was to examine the pharmacokinetics of a single dose (6.3 mumol, 3 mg) of all-trans retinoyl beta-glucuronide (RAG), when given either orally in corn oil or by intraperitoneal (IP) injection in dimethylsulfoxide (DMSO) to adult Sprague-Dawley rats. Following dosing, serial blood samples were collected at various times up to 48 hours from each rat via saphenous vein puncture. Retinoids were extracted from plasma samples and analyzed by high-performance liquid chromatography. In the plasma of IP-dosed rats (n = 6), a derivative of RAG, tentatively identified as the lactone of RAG (RAGL), was the major product found. RAGL persisted in the plasma for up to 48 hours. Much smaller concentrations of RAG and of retinoic acid (RA) were also present in the plasma at two to four hours, but generally not thereafter. In orally dosed rats (n = 6), neither RAG nor its products, except for occasional traces of the lactone, were detected. Plasma retinol levels decreased in both IP-injected and orally treated rats, the decrease being significant in orally dosed rats.

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Esterification of 5-Norbornene-2,3-Dicarboxylic Anhydride under Titanium Catalyst

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.634-638.642 ◽

2013 ◽

Vol 634-638 ◽

pp. 642-646 ◽

Cited By ~ 3

Author(s):

Sung Ho Park ◽

Jun Seong Park ◽

Tae Won Ko ◽

Yong Sung Park ◽

Je Wan Woo

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

1H Nmr ◽

High Performance ◽

Alder Reaction ◽

Diels Alder ◽

Titanium Catalyst ◽

Light Yellow ◽

Diels Alder Reaction ◽

Yield And Purity

Norbornene dicarboxylate was synthesized through esterification under titanium catalyst. The alcohols are 2-ethyl-1-hexanol, n-octanol, 3,5,5-trimethyl-1-hexanol, and n-decanol. The Structures of compounds were identified by 1H-NMR, and then components were identified by high performance liquid chromatography (HPLC). The yield and purity was over 90 % and 95 %, and color of compounds was colorless or light yellow. The maleate as a by-product was occured by the retro Diels-Alder reaction.

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Further characterization, by a combined high-performance liquid chromatography/1H-NMR approach, of the heterogeneity displayed by the neutral carbohydrate chains of human bronchial mucins

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1984.tb08363.x ◽

1984 ◽

Vol 143 (1) ◽

pp. 227-236 ◽

Cited By ~ 52

Author(s):

Genevieve LAMBLIN ◽

Arnold BOERSMA ◽

Michel LHERMITTE ◽

Philippe ROUSSEL ◽

Johanna MUTSAERS ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

1H Nmr ◽

High Performance ◽

Carbohydrate Chains

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Excretion of corticosteroid metabolites in urine and faeces of rats

Laboratory Animals ◽

10.1258/0023677011911886 ◽

2001 ◽

Vol 35 (4) ◽

pp. 307-314 ◽

Cited By ~ 52

Author(s):

E. Bamberg ◽

R. Palme ◽

J. G. Meingassner

Keyword(s):

High Performance Liquid Chromatography ◽

Diurnal Variation ◽

Intraperitoneal Injection ◽

High Performance ◽

Isotonic Saline ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Faecal Corticosterone Metabolites ◽

Low Levels ◽

Corticosterone Metabolites

Stress enhances the production of corticosteroids by the adrenal cortex, resulting in the increased excretion of their metabolites in urine and faeces. An intraperitoneal injection of radioactive corticosterone was applied to adult, male Sprague-Dawley rats to monitor the route and delay of excreted metabolites in urine and faeces. Peak concentrations appeared in urine after 3.2 ± 1.9 h and in faeces after 16.7 ± 4.3 h. Altogether about 20% of the recovered metabolites were found in urine and about 80% in faeces. Using high-performance liquid chromatography (HPLC), several peaks of radioactive metabolites were found. Some metabolites were detected by enzyme immunoassay (EIA) using two different antibodies (corticosterone, 11β-OH-aetiocholanolone). There was a marked diurnal variation with low levels of faecal corticosterone metabolites in the evening and higher values in the morning. This diurnal variation was influenced neither by the intraperitoneal injection of isotonic saline nor by ACTH. However, the administration of dexamethasone eliminated the morning peak for 2 days.

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