Platelet-Derived Growth Factor-BB Inhibits Rat α1D-Adrenergic Receptor Gene Expression in Vascular Smooth Muscle Cells by Inducing AP-2-Like Protein Binding to α1D Proximal Promoter Region

1999 ◽  
Vol 56 (6) ◽  
pp. 1152-1161 ◽  
Author(s):  
Xiaohua Xin ◽  
Nengyu Yang ◽  
James E. Faber
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Adrenergic Receptor ◽  
Promoter Region ◽  
Receptor Gene ◽  
Platelet Derived Growth Factor ◽  
Proximal Promoter ◽  
Receptor Gene Expression ◽  
Adrenergic Receptor Gene

Platelet-derived growth factor and growth-related genes in rat lung. II. Effect of exposure to 85% O2

1992 ◽  
Vol 262 (2) ◽  
pp. L140-L146 ◽  
Author(s):  
R. N. Han ◽  
S. Buch ◽  
B. A. Freeman ◽  
M. Post ◽  
A. K. Tanswell
Keyword(s):  
Gene Expression ◽  
Cell Division ◽  
Growth Factor ◽  
Receptor Gene ◽  
Platelet Derived Growth Factor ◽  
Chain Gene ◽  
Transcription Analysis ◽  
A Chain ◽  
Type Receptor ◽  
Receptor Gene Expression

The expression of platelet-derived growth factor (PDGF), its receptor, and related genes was studied in the lung tissue of rats exposed to air or 85% O2. PDGF-B chain mRNA was increased by 6 days and PDGF B-type receptor mRNA was increased by 4 and 6 days of exposure to 85% O2. Despite a continued increase of cell division, both PDGF-B chain and B-type receptor mRNAs had returned to control values by 14 days of exposure to 85% O2. PDGF-A chain mRNA was unaffected by exposure to 85% O2. Nuclear runoff transcription analysis confirmed increased transcription of PDGF-B chain mRNA, whereas Western blot analysis of lung homogenates suggested consequent increased translation of PDGF-B chain mRNA to PDGF-BB at 7 days of exposure to 85% O2. Combined immunocytochemistry and autoradiography localized PDGF-BB to the major site of cell division, the pulmonary interstitium. We speculate that the early pulmonary fibroblast hyperplasia observed following exposure to 85% O2 is mediated by increased PDGF-B chain gene expression and may also be mediated by changes in PDGF B-type receptor gene expression.

Protein Kinase C-Mediated Down-Regulation of β1-Adrenergic Receptor Gene Expression in Rat C6 Glioma Cells

1998 ◽  
Vol 54 (1) ◽  
pp. 14-21 ◽  
Author(s):  
Zhongwei Li ◽  
Vidita A. Vaidya ◽  
John D. Alvaro ◽  
Philip A. Iredale ◽  
Richard Hsu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adrenergic Receptor ◽  
Receptor Gene ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C ◽  
Receptor Gene Expression ◽  
Adrenergic Receptor Gene ◽  
Rat C6 Glioma

scholarly journals Altered Beta-2 Adrenergic Receptor Gene Expression in Human Clinical Hypertension

2009 ◽  
Vol 11 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Jennifer R. Dungan ◽  
Yvette P. Conley ◽  
Taimour Y. Langaee ◽  
Julie A. Johnson ◽  
Shawn M. Kneipp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Coronary Artery ◽  
Coronary Artery Bypass ◽  
Adrenergic Receptor ◽  
Artery Bypass ◽  
Receptor Gene ◽  
Positive Family History ◽  
Beta 2 ◽  
Receptor Gene Expression ◽  
Adrenergic Receptor Gene

Objectives: The beta-2 adrenergic receptor is involved in mediating vasodilatation via neurohumoral and sympathetic nervous system pathways. Alterations in beta-2 adrenergic receptor gene expression (mRNA transcription) may contribute to the hypertensive phenotype. Human gene expression in clinical phenotypes remains largely unexplored due to ethical constraints involved in obtaining human tissue. We devised a method to obtain normally discarded internal mammary artery tissue from coronary artery bypass graft patients. We then investigated differences in hypertensive and normotensive participants' beta-2 adrenergic receptor gene expression in this tissue. Methods: We collected arterial tissue samples from 46 coronary artery bypass patients in a surgical setting. Using 41 of the samples, we performed TaqMan real-time polymerase chain reaction (RT-PCR) and used the delta delta cycle threshold (ΔΔCt) relative quantitation method for determination of fold-differences in gene expression between normotensive and hypertensive participants. The beta-2 adrenergic receptor target was normalized to glyceraldehyde-phosphate dehydrogenase. Results: Participants with hypertension had significantly less-expressed beta-2 adrenoceptor gene (2.76-fold, p < .05) compared to normotensive participants. After Bonferroni correction, gene expression did not differ by race, gender, type/dose of β-blocker prescribed, positive family history of hypertension, or diagnosis of diabetes mellitus type 2. Conclusions: These data support the possibility of a molecular basis for impaired adrenoceptor-mediated vascular tone in hypertension. Modification and extension of this research is required.

Adrenergic Regulation of ICER (Inducible Cyclic AMP Early Repressor) and β1-Adrenergic Receptor Gene Expression in C6 Glioma Cells

2002 ◽  
Vol 67 (2) ◽  
pp. 490-497 ◽  
Author(s):  
Laura Rydelek Fitzgerald ◽  
Zhongwei Li ◽  
Curtis A. Machida ◽  
Peter H. Fishman ◽  
Ronald S. Duman
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Adrenergic Receptor ◽  
Receptor Gene ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Adrenergic Regulation ◽  
Receptor Gene Expression ◽  
Adrenergic Receptor Gene
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