Multi-stage controllable degradation of strontium-doped calcium sulfate hemihydrate-Tricalcium phosphate microsphere composite as a substitute for osteoporotic bone defect repairing: Degradation behavior and bone response

2021 ◽  
Author(s):  
Qiuju Miao ◽  
Nan Jiang ◽  
Qinmeng Yang ◽  
Ismail mohamed Hussein ◽  
Zhen Luo ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Calcium Sulfate ◽  
Bone Repair ◽  
Degradation Rate ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
Repair Materials ◽  
Bone Repair Materials ◽  
In Vivo Degradation

Abstract Various requirements for the repair of complex bone defects have motivated to development of scaffolds with adjustable degradation rates and biological functions. Tricalcium phosphate and calcium sulfate are the most commonly used bone repair materials in the clinic, how to better combine tricalcium phosphate and calcium sulfate and play their greatest advantages in the repair of osteoporotic bone defect is the focus of our research. In this study, a series of scaffolds with multistage-controlled degradation properties composed of strontium-doped calcium sulfate (SrCSH) and strontium-doped tricalcium phosphate microspheres (Sr-TCP) scaffolds were prepared, and their osteogenic activity, in vivo degradation and bone regeneration ability in tibia of osteoporotic rats were evaluated. In vitro studies revealed that different components of SrCSH/Sr-TCP scaffolds significantly promoted the proliferation and differentiation of MC3T3-E1 cells, which showed a good osteogenic induction activity. In vivo degradation results showed that the degradation time of composite scaffolds could be controlled in a large range (6-12 months) by controlling the porosity and phase composition of Sr-TCP microspheres. The results of osteoporotic femoral defect repair showed that when the degradation rate of scaffold matched with the growth rate of new bone, the parameters such as BMD, BV/TV, Tb.Th, angiogenesis marker CD31 and new bone formation marker OCN expression were higher, which promoted the rapid repair of osteoporotic bone defects. On the contrary, the slow degradation rate of scaffolds hindered the growth of new bone to a certain extent. This study elucidates the importance of the degradation rate of scaffolds for the repair of osteoporotic bone defects, and the design considerations can be extended to other bone repair materials, which is expected to provide new ideas for the development of tissue engineering materials in the future.

scholarly journals A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

2020 ◽  
Vol 6 (50) ◽  
pp. eabc4704
Author(s):  
Bo Yuan ◽  
Linnan Wang ◽  
Rui Zhao ◽  
Xi Yang ◽  
Xiao Yang ◽  
...  
Keyword(s):  
Bone Repair ◽  
Osteoporotic Fractures ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Worldwide Population ◽  
Repair Materials ◽  
Porous Architecture ◽  
Viable Approach

Osteoporotic fractures are prevalent in society, and their incidence appears to be increasing as the worldwide population ages. However, conventional bone repair materials hardly satisfy the requirements for the repair of pathological fractures. Here, we developed a biomimetic polyetherketoneketone scaffold with a functionalized strontium-doped nanohydroxyapatite coating for osteoporotic bone defect applications. The scaffold has a hierarchically porous architecture and mechanical strength similar to that of osteoporotic trabecular bone. In vitro and in vivo studies demonstrated that the scaffold could promote osteoporotic bone regeneration and delay adjacent bone loss via regulating both osteoblasts and osteoclasts. In addition, the correlations between multiple preimplantation and postimplantation parameters were evaluated to determine the potential predictors of in vivo performance of the material. The current work not only develops a promising candidate for osteoporotic bone repair but also provides a viable approach for designing other functional biomaterials and predicting their translational value.

scholarly journals Fe/Zn-modified tricalcium phosphate (TCP) biomaterials: preparation and biological properties

RSC Advances ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 781-789 ◽  
Author(s):  
Lu Xie ◽  
Yuanyi Yang ◽  
Zhiqiang Fu ◽  
Yunfei Li ◽  
Jiacheng Shi ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Biological Properties ◽  
Synthetic Bone ◽  
Repair Materials ◽  
Bone Repair Materials

Fe/Zn-TCP biomaterials were prepared and their bioactivities to enhance the synthetic bone-repair materials were studied in comparison.

scholarly journals In vivo changes of nanoapatite crystals during bone reconstruction and the differences with native bone apatite

2019 ◽  
Vol 5 (11) ◽  
pp. eaay6484 ◽  
Author(s):  
Xiyu Li ◽  
Qin Zou ◽  
Haifeng Chen ◽  
Wei Li
Keyword(s):  
Bone Tissue ◽  
Bone Repair ◽  
Crystal Size ◽  
Bone Reconstruction ◽  
Native Bone ◽  
Future Design ◽  
Repair Materials ◽  
Bone Repair Materials ◽  
Bone Apatite

Hydroxyapatite (HA) plays an important role in clinical bone repair. However, it remains a challenge to accurately determine its changes during bone reconstruction and to identify its differences from native bone apatite. Here, terbium (Tb) doped uniform HA nanocrystals were implanted into bone tissue and compared with native bone apatite. These comparisons demonstrated the occurrence of compositional and structural alteration of the implanted HA nanocrystals, and their gradual degradation, during bone reconstruction. They also revealed notable differences between HA nanocrystals and bone apatite crystals in crystal size, distribution pattern, and state of existence in bone tissue. Although synthetic HA nanocrystals could osteointegrate with bone tissue, they still seemed to be treated as foreign material in this tissue and thus were gradually degraded. These findings can help to identify and rethink the function of synthetic apatite and bone apatite, which will benefit future design and application of biomimetic bone repair materials.

scholarly journals Synthesis and properties of Sr2+ doping α-tricalcium phosphate at low temperature

2021 ◽  
Vol 19 ◽  
pp. 228080002199699
Author(s):  
Zhen Yuan ◽  
Jianqiang Bi ◽  
Weili Wang ◽  
Xiaoning Sun ◽  
Lu Wang ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Low Temperature ◽  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Setting Time ◽  
Cell Activity ◽  
Repair Materials ◽  
Calcium Phosphate Bone Cement ◽  
Bone Repair Materials ◽  
Osteoclast Apoptosis

Strontium has been widely used in bone repair materials due to its roles in promoting osteoclast apoptosis and enhancing osteoblast proliferation. In this work, synthesis and the effects of Sr2+ doping α-tricalcium phosphate at low-temperature was studied. The setting time and the mechanical properties of α-tricalcium phosphate were controlled by varying the content of Sr2+. The synthesized compounds were evaluated by XRD, SEM, XPS, setting time, compressive strength, SBF immersion, and colorimetric CCK-8 assay. The results showed that Sr2+ can improve the compressive strength and cell activity of calcium phosphate bone cement.

Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study

2019 ◽  
Vol 70 (6) ◽  
pp. 1983-1987
Author(s):  
Cristian Trambitas ◽  
Anca Maria Pop ◽  
Alina Dia Trambitas Miron ◽  
Dorin Constantin Dorobantu ◽  
Flaviu Tabaran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Bone Repair ◽  
Bone Defects ◽  
Calvarial Bone ◽  
Specific Protocol ◽  
Repair Mechanisms ◽  
Male Wistar Rats

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

scholarly journals Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nan Jiang ◽  
Devendra H. Dusane ◽  
Jacob R. Brooks ◽  
Craig P. Delury ◽  
Sean S. Aiken ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Calcium Sulfate ◽  
Bone Graft Substitute ◽  
In Vitro Assays ◽  
In Vivo Experiments ◽  
Orthopaedic Infections ◽  
Clinical Investigations ◽  
Antimicrobial Potency

AbstractThis study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa (PAO1) and S. aureus (SAP231) biofilms in in vitro assays. Antibiotic loaded β-TCP/CS beads were compared with antibiotic loaded beads formed from a well characterized synthetic calcium sulfate (CS) bone void filler. β-TCP/CS antibiotic loaded showed antimicrobial potency against PAO1 in a repeated Kirby-Bauer like zone of inhibition assay for 6 days compared to 8 days for CS. However, both bead types showed potency against SAP231 for 40 days. Both formulations loaded with V + T completely prevented biofilm formation (CFU below detection limits) for the 3 days of the experiment with daily fresh inoculum challenges (P < 0.001). In addition, both antibiotic loaded materials and antibiotic combinations significantly reduced the bioburden of pre-grown biofilms by between 3 and 5 logs (P < 0.001) with V + G performing slightly better against PAO1 than V + T. Our data, combined with previous data on osteogenesis suggest that antibiotic loaded β-TCP/CS may have potential to stimulate osteogenesis through acting as a scaffold as well as simultaneously protecting against biofilm infection. Future in vivo experiments and clinical investigations are warranted to more comprehensively evaluate the use of β-TCP/CS in the management of orthopaedic infections.

In Vitro and In Vivo Degradation, Mechanical Properties, and Histocompatibility of Weft-Knitted Silk Mesh-Like Grafts

2022 ◽  
Vol 12 (2) ◽  
pp. 411-416
Author(s):  
Liang Tang ◽  
Si-Yu Zhao ◽  
Ya-Dong Yang ◽  
Geng Yang ◽  
Wen-Yuan Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Degradation Rate ◽  
Maximum Load ◽  
Artificial Ligament ◽  
In Vivo Degradation

To investigate the degradation, mechanical properties, and histocompatibility of weft-knitted silk mesh-like grafts, we carried out the In Vitro and In Vivo silk grafts degradation assay. The In Vitro degradation experiment was performed by immersing the silk grafts in simulated body fluid for 1 year, and the results showed that the degradation rate of the silk mesh-like grafts was very slow, and there were few changes in the mechanical properties and quality of the silk mesh-like graft. In Vivo degradation assay was taken by implantation of the silk mesh-like grafts into the subcutaneous muscles of rabbits. At 3, 6, and 12 months postoperation, the rate of mass loss was 19.36%, 31.84%, and 58.77%, respectively, and the maximum load was 63.85%, 34.63%, and 10.76%, respectively of that prior to degradation. The results showed that the degradation rate of the silk graft and the loss of mechanical properties In Vivo were faster than the results obtained in the In Vitro experiments. In addition, there were no significant differences in secretion of serum IL-6 and TNF-α between the experimental and normal rabbits (P >0.05), suggesting no obvious inflammatory reaction. The findings suggest that the weft-knitted silk mesh-like grafts have good mechanical properties, histocompatibility, and In Vivo degradation rate, and therefore represent a candidate material for artificial ligament

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