Formation of α-Keto Acids from α-Amino Acids by the Action of Free Radicals in Aqueous Solution

Science ◽  
1951 ◽  
Vol 114 (2964) ◽  
pp. 412-413
Author(s):  
G. R. A. Johnson ◽  
George Scholes ◽  
Joseph Weiss
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Free Radicals ◽  
Keto Acids

Formation of α-Keto Acids from α-Amino Acids by the Action of Free Radicals in Aqueous Solution

Science ◽  
1951 ◽  
Vol 114 (2964) ◽  
pp. 412-413
Author(s):  
G. R. A. Johnson ◽  
George Scholes ◽  
Joseph Weiss
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Free Radicals ◽  
Keto Acids

Formation of agr-Keto Acids from agr-Amino Acids by the Action of Free Radicals in Aqueous Solution

Science ◽  
1951 ◽  
Vol 114 (2964) ◽  
pp. 412-413 ◽  
Author(s):  
G. R. A. Johnson ◽  
G. Scholes ◽  
J. Weiss
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Free Radicals ◽  
Keto Acids

A colorimetric chemosensor for the sequential detection of copper ion and amino acids (cysteine and histidine) in aqueous solution

RSC Advances ◽  
2015 ◽  
Vol 5 (39) ◽  
pp. 31179-31188 ◽  
Author(s):  
Yong Sung Kim ◽  
Gyeong Jin Park ◽  
Seul Ah Lee ◽  
Cheal Kim
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Copper Ion ◽  
Sequential Detection ◽  
Colorimetric Chemosensor

A simple and selective colorimetric chemosensor was reported for the sequential detection of Cu2+, cysteine and histidine.

scholarly journals ω-Amino Acid:Pyruvate Transaminase from Alcaligenes denitrificans Y2k-2: a New Catalyst for Kinetic Resolution of β-Amino Acids and Amines

2004 ◽  
Vol 70 (4) ◽  
pp. 2529-2534 ◽  
Author(s):  
Hyungdon Yun ◽  
Seongyop Lim ◽  
Byung-Kwan Cho ◽  
Byung-Gee Kim
Keyword(s):  
Amino Acids ◽  
Kinetic Resolution ◽  
Specific Activity ◽  
Expression System ◽  
Enantiomeric Excess ◽  
Selective Enrichment ◽  
Alcaligenes Denitrificans ◽  
Keto Acids ◽  
Optically Pure ◽  
High Stereoselectivity

ABSTRACT Alcaligenes denitrificans Y2k-2 was obtained by selective enrichment followed by screening from soil samples, which showed ω-amino acid:pyruvate transaminase activity, to kinetically resolve aliphatic β-amino acid, and the corresponding structural gene (aptA) was cloned. The gene was functionally expressed in Escherichia coli BL21 by using an isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible pET expression system (9.6 U/mg), and the recombinant AptA was purified to show a specific activity of 77.2 U/mg for l-β-amino-n-butyric acid (l-β-ABA). The enzyme converts various β-amino acids and amines to the corresponding β-keto acids and ketones by using pyruvate as an amine acceptor. The apparent Km and V max for l-β-ABA were 56 mM and 500 U/mg, respectively, in the presence of 10 mM pyruvate. In the presence of 10 mM l-β-ABA, the apparent Km and V max for pyruvate were 11 mM and 370 U/mg, respectively. The enzyme exhibits high stereoselectivity (E > 80) in the kinetic resolution of 50 mM d,l-β-ABA, producing optically pure d-β-ABA (99% enantiomeric excess) with 53% conversion.

Physico-chemical Characteristics of Amino Acids in Aqueous Solution of Amikacin Sulphate

2021 ◽  
Author(s):  
Poonam Chaudhary ◽  
◽  
Suvarcha Chauhan ◽  
Vivek Sharma ◽  
Kuldeep Singh ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Chemical Characteristics ◽  
Physico Chemical

Free Amino Acids in Citrullus vulgaris (Watermelon)

PEDIATRICS ◽  
1984 ◽  
Vol 73 (6) ◽  
pp. 879-879
Author(s):  
T. A. TEDESCO ◽  
S. A. BENFORD ◽  
R. C. FOSTER ◽  
L. A. BARNESS
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Neonatal Period ◽  
Essential Amino Acids ◽  
Solid Foods ◽  
Citrullus Vulgaris ◽  
Keto Acids ◽  
Wet Weight ◽  
Cycle Disorders

To the Editor.— Currently accepted dietary management of citrullinemia and other urea cycle disorders includes protein restriction, sodium benzoate, and dietary supplements of keto acids or essential amino acids with postblock intermediates such as arginine in citrullinemia and arginino-succinic aciduria. When a child survives the neonatal period on such a regimen and solid foods are introduced into the diet, there is at least one fruit that should be avoided, Citrullus Vulgaris, commonly known as watermelon. Quantitation of free amino acids extracted from 1 g wet weight of watermelon fruit yielded the following (in mmoles per gram wet weight): Phenylalanine, 1.25; histidine, 0.24; tryptophan, 0.35; lysine, 0.82; ornithine, 0.32; arginine, 11.36; aspartic acid, 0.97; threonine, 0.74; serine, 1.05; glutamine, 3.86; glutamic acid, 1.38; citrulline, 23.68; alanine, 1.15; valine, 0.17; isoleucine, 1.24; leucine, 0.24.

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