scholarly journals A Tissue-Like Printed Material

Science ◽  
2013 ◽  
Vol 340 (6128) ◽  
pp. 48-52 ◽  
Author(s):  
Gabriel Villar ◽  
Alexander D. Graham ◽  
Hagan Bayley
Keyword(s):  
Tissue Engineering ◽  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Collective Behavior ◽  
Three Dimensional ◽  
Living Cells ◽  
Emergent Properties ◽  
Living Tissue ◽  
Cohesive Material

Living cells communicate and cooperate to produce the emergent properties of tissues. Synthetic mimics of cells, such as liposomes, are typically incapable of cooperation and therefore cannot readily display sophisticated collective behavior. We printed tens of thousands of picoliter aqueous droplets that become joined by single lipid bilayers to form a cohesive material with cooperating compartments. Three-dimensional structures can be built with heterologous droplets in software-defined arrangements. The droplet networks can be functionalized with membrane proteins; for example, to allow rapid electrical communication along a specific path. The networks can also be programmed by osmolarity gradients to fold into otherwise unattainable designed structures. Printed droplet networks might be interfaced with tissues, used as tissue engineering substrates, or developed as mimics of living tissue.

Tough Double-Network Hydrogels as Scaffolds for Tissue Engineering

2012 ◽  
pp. 213-222
Author(s):  
Jing Jing Yang ◽  
Jian Fang Liu ◽  
Takayuki Kurokawa ◽  
Nobuto Kitamura ◽  
Kazunori Yasuda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Regeneration ◽  
Cell Types ◽  
Embryoid Bodies ◽  
Living Tissue ◽  
Double Network ◽  
Dimensional Network

Hydrogels are used as scaffolds for tissue engineering in vitro & in vivo because their three-dimensional network structure and viscoelasticity are similar to those of the macromolecular-based extracellular matrix (ECM) in living tissue. Especially, the synthetic hydrogels with controllable and reproducible properties were used as scaffolds to study the behaviors of cells in vitro and implanted test in vivo. In this review, two different structurally designed hydrogels, single-network (SN) hydrogels and double-network (DN) hydrogels, were used as scaffolds. The behavior of two cell types, anchorage-dependent cells and anchorage-independent cells, and the differentiation behaviors of embryoid bodies (EBs) were investigated on these hydrogels. Furthermore, the behavior of chondrocytes on DN hydrogels in vitro and the spontaneous cartilage regeneration induced by DN hydrogels in vivo was examined.

Laser-based 3D cell printing for tissue engineering

2014 ◽  
Vol 15 (3-4) ◽  
Author(s):  
Lothar Koch ◽  
Andrea Deiwick ◽  
Boris Chichkov
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Three Dimensional ◽  
Living Cells ◽  
Cell Printing ◽  
Printing Technique ◽  
Cell Patterns ◽  
Printing Techniques

AbstractCurrently, different 3D printing techniques are investigated for printing biomaterials and living cells. An ambitious aim is the printing of fully functional tissue or organs. Furthermore, for manifold applications in biomedical research and in testing of pharmaceuticals or cosmetics, printed tissue could be a new method, partly substituting test animals. Here we describe a laser-based printing technique applied for the arrangement of vital cells in two and three-dimensional patterns and for tissue engineering. First printed tissue, tested in vitro and in vivo, and printing of cell patterns for investigating cell-cell interactions are presented.

scholarly journals Watching Three-Dimensional Movements of Single Membrane Proteins in Lipid Bilayers

Biochemistry ◽  
2018 ◽  
Vol 57 (31) ◽  
pp. 4735-4740 ◽  
Author(s):  
Li Ma ◽  
Ying Li ◽  
Jianbing Ma ◽  
Shuxin Hu ◽  
Ming Li
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Three Dimensional ◽  
Single Membrane

The effect of hydrodynamic interactions on the tracer and gradient diffusion of integral membrane proteins in lipid bilayers

1994 ◽  
Vol 258 ◽  
pp. 167-190 ◽  
Author(s):  
Stuart J. Bussell ◽  
Daniel A. Hammer ◽  
Donald L. Koch
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Stokes Equations ◽  
Thin Sheet ◽  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Area Fraction ◽  
Hydrodynamic Interactions ◽  
Low Viscosity ◽  
Long Time

Biological membranes can be considered two-dimensional fluids with suspended integral membrane proteins (IMPs). We have calculated the effect of hydrodynamic interactions on the various diffusion coefficients of IMPs in lipid bilayers. The IMPs are modelled as hard cylinders of radius a immersed in a thin sheet of viscosity μ and thickness h bounded by a fluid of low viscosity μ′. We have ensemble averaged the N-body Stokes equations to the pair level and have renormalized them following the methods of Batchelor (1972) and Hinch (1977). The lengthscale for the hydrodynamic interactions is λa = μh / μ′, Which is O (100a), and the slow decay of the interactions introduces new features in the renormalizations compared to the analogous analyses for three-dimensional suspensions of spheres.We have calculated the asymptotic limits for the short- and long-time tracer diffusivities, Ds and Dl, respectively, and for the gradient diffusivity, Dg, for ϕ [Lt ] 1 and λ [Gt ] 1, where ϕ is the IMP area fraction and λ = μh / (μ′a). The diffusivities are \begin{eqnarray*} D_s/D_0 &=& 1-2\phi[1-(1+\ln (2)-9/32)/(\ln(\lambda)-\gamma)], D_l/D_0 &=& D_s/D_0 - 0.07/(\ln(\lambda)-\gamma), D_g/D_0 &=& 1+\phi[-7+(6\ln(2)+7/16+0.37)/(\ln(\lambda)-\gamma)], \end{eqnarray*} where D0 is the diffusivity in the limit of zero area fraction, and γ = 0.577216 is Euler's constant. The results for Dl and Ds differ only slightly. The decrease in Dg/Do as ϕ increases contrasts with the result for spheres for which Dg/Do > 1.

scholarly journals Editorial for the Special Issue on 3D Printing for Tissue Engineering and Regenerative Medicine

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 366 ◽  
Author(s):  
Vahid Serpooshan ◽  
Murat Guvendiren
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Three Dimensional ◽  
Living Cells ◽  
3D Bioprinting ◽  
Special Issue ◽  
3D Structures ◽  
Manufacturing Techniques

Three-dimensional (3D) bioprinting uses additive manufacturing techniques to fabricate 3D structures consisting of heterogenous selections of living cells, biomaterials, and active biomolecules [...]

scholarly journals Bioprinting of tissue engineering scaffolds

2018 ◽  
Vol 9 ◽  
pp. 204173141880209 ◽  
Author(s):  
Patrick Rider ◽  
Željka Perić Kačarević ◽  
Said Alkildani ◽  
Sujith Retnasingh ◽  
Mike Barbeck
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Three Dimensional ◽  
Living Cells ◽  
Tissue Engineering Scaffolds ◽  
Technical Challenges ◽  
Manufacturing Techniques

Bioprinting is the process of creating three-dimensional structures consisting of biomaterials, cells, and biomolecules. The current additive manufacturing techniques, inkjet-, extrusion-, and laser-based, create hydrogel structures for cellular encapsulation and support. The requirements for each technique, as well as the technical challenges of printing living cells, are discussed and compared. This review encompasses the current research of bioprinting for tissue engineering and its potential for creating tissue-mimicking structures.

scholarly journals Current Advances in 3D Bioprinting Technology and Its Applications for Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2958
Author(s):  
JunJie Yu ◽  
Su A Park ◽  
Wan Doo Kim ◽  
Taeho Ha ◽  
Yuan-Zhu Xin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Cell Density ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Living Cells ◽  
3D Bioprinting ◽  
3D Objects ◽  
Advantages And Disadvantages ◽  
Resolution Cell

Three-dimensional (3D) bioprinting technology has emerged as a powerful biofabrication platform for tissue engineering because of its ability to engineer living cells and biomaterial-based 3D objects. Over the last few decades, droplet-based, extrusion-based, and laser-assisted bioprinters have been developed to fulfill certain requirements in terms of resolution, cell viability, cell density, etc. Simultaneously, various bio-inks based on natural–synthetic biomaterials have been developed and applied for successful tissue regeneration. To engineer more realistic artificial tissues/organs, mixtures of bio-inks with various recipes have also been developed. Taken together, this review describes the fundamental characteristics of the existing bioprinters and bio-inks that have been currently developed, followed by their advantages and disadvantages. Finally, various tissue engineering applications using 3D bioprinting are briefly introduced.

Microfluidic fabrication of cell-derived nanovesicles as endogenous RNA carriers

Lab on a Chip ◽  
2014 ◽  
Vol 14 (7) ◽  
pp. 1261-1269 ◽  
Author(s):  
Wonju Jo ◽  
Dayeong Jeong ◽  
Junho Kim ◽  
Siwoo Cho ◽  
Su Chul Jang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Living Cells ◽  
Plasma Membrane Proteins ◽  
Biological Signal

Artificial exosomes of ~100 nm diameter, enclosed with lipid bilayers, are fabricated from living cells and transfer biological signal components such as encapsulated RNAs and proteins, plasma membrane proteins, or both.

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