scholarly journals Slow Release of Nitric Oxide from Charged Catheters and Its Effect on Biofilm Formation by Escherichia coli

2009 ◽  
Vol 54 (1) ◽  
pp. 273-279 ◽  
Author(s):  
Gilly Regev-Shoshani ◽  
Mary Ko ◽  
Chris Miller ◽  
Yossef Av-Gay
Keyword(s):  
Nitric Oxide ◽  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Biofilm Formation ◽  
Slow Release ◽  
Bacterial Colonization ◽  
Bactericidal Effect ◽  
Urinary Catheters ◽  
Bacterial Concentration

ABSTRACT Catheter-associated urinary tract infection is the most prevalent cause of nosocomial infections. Bacteria associated with biofilm formation play a key role in the morbidity and pathogenesis of these infections. Nitric oxide (NO) is a naturally produced free radical with proven bactericidal effect. In this study, Foley urinary catheters were impregnated with gaseous NO. The catheters demonstrated slow release of nitric oxide over a 14-day period. The charged catheters were rendered antiseptic, and as such, were able to prevent bacterial colonization and biofilm formation on their luminal and exterior surfaces. In addition, we observed that NO-impregnated catheters were able to inhibit the growth of Escherichia coli within the surrounding media, demonstrating the ability to eradicate a bacterial concentration of up to 104 CFU/ml.

scholarly journals Uropathogenic Escherichia coli-Induced Inflammation Alters Mouse Urinary Bladder Contraction via an Interleukin-6-Activated Inducible Nitric Oxide Synthase-Related Pathway

2009 ◽  
Vol 77 (8) ◽  
pp. 3312-3319 ◽  
Author(s):  
Te I. Weng ◽  
Hsiao Yi Wu ◽  
Pei Ying Lin ◽  
Shing Hwa Liu
Keyword(s):  
Nitric Oxide ◽  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Urinary Bladder ◽  
Tract Infection ◽  
Interleukin 6 ◽  
Electrical Field Stimulation ◽  
E Coli

ABSTRACT Escherichia coli is the most common cause of urinary tract infection. Elevated blood and urine interleukin-6 (IL-6) levels have been shown in inflammatory urinary tract diseases. The role of IL-6 in mediating the urodynamic dysfunction in response to E. coli-induced urinary tract infection has not yet been fully elucidated. In this study, we investigated the role of IL-6 in the nitric oxide (NO)-triggered alteration of contractile responses in the urinary bladder under an E. coli-induced inflammatory condition. The electrical field stimulation (EFS)-evoked contractions of the isolated detrusor strips, and immunoblotting for detecting protein expression in the bladders was measured short term (1 h) or long term (6 or 24 h) after intraperitoneal injection of E. coli endotoxin (lipopolysaccharide [LPS]) or intravesical instillation of human pyelonephritogenic E. coli-J96 (O4:K6) strain or LPS into mice. IL-6 and NO productions were increased in the urinary bladders of mice 1 to 24 h after LPS or E. coli-J96 treatment. Inducible NO synthase (iNOS) expression and protein kinase C (PKC) activation and EFS-evoked detrusor contractions were increased in the bladders at 6 h after LPS or E. coli-J96 treatment, which could be reversed by anti-IL-6 antibody and iNOS inhibitor aminoguanidine. At 1 h after LPS administration, bladder NO generation, endothelial NOS expression, and EFS-evoked detrusor contractions were effectively increased, whereas anti-IL-6 antibody could not reverse these LPS-induced responses. These results indicate that IL-6 may play an important role in the iNOS/NO-triggered PKC-activated contractile response in urinary bladder during E. coli or LPS-induced inflammation.

Polymicrobial Interactions in the Urinary Tract: Is the Enemy of My Enemy My Friend?

2021 ◽  
Vol 89 (4) ◽  
Author(s):  
Jordan R. Gaston ◽  
Alexandra O. Johnson ◽  
Kirsten L. Bair ◽  
Ashley N. White ◽  
Chelsie E. Armbruster
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Catheters ◽  
Content Type

The vast majority of research pertaining to urinary tract infection has focused on a single pathogen in isolation and predominantly Escherichia coli . However, polymicrobial urine colonization and infections are prevalent in several patient populations, including individuals with urinary catheters.

scholarly journals Prevalence of microorganisms causing catheter associated urinary tract infections (CAUTI) among catheterised patients admitted in a tertiary care hospital

2017 ◽  
Vol 5 (6) ◽  
pp. 2367 ◽  
Author(s):  
Vinoth M. ◽  
Prabagaravarthanan R. ◽  
Bhaskar M.
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Bacterial Colonization ◽  
Tertiary Care ◽  
Age Groups ◽  
Care Hospital ◽  
Cross Sectional ◽  
Tract Infections

Background: Catheter-associated urinary tract infection (CAUTI) is an important cause of morbidity and mortality in Indian subjects, affecting all age groups. Bacteriuria orcandiduria is almost inevitable in nearly half of the patients who require an indwelling urinary catheter for more than 5 days. The objective of this study was to find the prevalence of CAUTI infection and to determine their antibiotic profileMethods: The present cross-sectional study included 50 patients admitted to medicine and surgery wards in our hospital. The urine from catheter is collected from each patient and subjected to culture and antibiotic susceptibility testing.Results: The prevalence of catheter associated urinary tract infection in our hospital is about 20% and asymptomatic bacterial colonization is 50% which is nearequal to Danchaivijitr S et al study. The common pathogens found in this study are Escherichia coli (22%), Klebsiella (18%), Enterobacter (8%), Staph. aureus (6%) which include MRSA (4%), Pseudomonas (6%), Enterococcus (4%), Candida sps. (4%) Andproteus (2%).Conclusions: CAUTI has low prevalence 20% and asymptomatic colonisation 50% in our hospital with common pathogen being Escherichia coli. It is one of the important notable pathogen causing nosocomial infection among admitted patients. The patients present mainly as asymptomatic bacterial colonisation and risk of CAUTI increases with longer duration of catheterisation. All patients those who had catheter for more than 6 days, aged 60 and above, should be checked for UTI symptoms. And their urine should be cultured regularly in order to diagnose and prevent CAUTI and its complications which are very dangerous and difficult to treat.

scholarly journals Pathogenic potential in catheter associated Escherichia coli is associated with separable biofilm and virulence gene determinants

2021 ◽  
Author(s):  
Zongsen Zou ◽  
Robert Potter ◽  
William McCoy ◽  
George Katumba ◽  
Peter J. Mucha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Biofilm Formation ◽  
Gene Networks ◽  
Bacterial Colonization ◽  
Asymptomatic Bacteriuria ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Pathogenic Potential ◽  
E Coli

Urinary catheterization facilitates asymptomatic bacterial colonization of the urinary tract and increases the risk of urinary tract infection (UTI). Differentiating catheter-associated asymptomatic bacteriuria (CAASB) from catheter-associated UTI (CAUTI) can be challenging when the presence or origins of symptoms are unclear. To determine whether strain-specific Escherichia coli characteristics are associated with CAUTI, we compared genomic composition and experimental catheter biofilm production by urinary isolates from catheterized inpatients and rectal isolates from healthy volunteers. CAUTI isolates were associated with a distinctive phylotype B2 sub-clade dominated by the multidrug resistant, pandemic ST131 lineage. While catheter biofilm formation was widespread among E. coli isolates, phylotype B2 biofilms were more extensive and biofilm-associated genes were preferentially found in B2 and ST131 isolates. Distinctive suites of iron-responsive genes were associated with both the ST131 lineage and catheter biofilm formation. Catheter biofilms produced by some CAASB strains could inhibit ST131 colonization, suggesting a potential beneficial function for these strains. These results suggest that the combination of biofilm and non-biofilm-associated gene networks in urinary E. coli influence CAUTI risk in catheterized patients.

scholarly journals Evaluation of antimicrobial effects of Rosemary and Withania somnifera methanol extract prepared by ultrasound waveform on Escherichia coli biofilm isolated from urinary tract infection

2021 ◽  
Vol 1 (01) ◽  
pp. 17-25
Author(s):  
Maryam Beigomi ◽  
Asiyeh Biabangard ◽  
Reza Rohani
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Biofilm Formation ◽  
Withania Somnifera ◽  
Significance Level ◽  
Antimicrobial Effects ◽  
E Coli ◽  
Significant Difference

Generally, bacteria co-exist setting up communities associated to solid superficies, this is to which we refer as biofilms that serve as a survival strategy. This type of formation cause serious sanitary problems for both humans and animals. Nowadays, chemical or natural compounds able to block this formation are looked for. In this project, we have evaluation of antimicrobial effects of Rosemary and Withania somnifera extract prepared by ultrasound waveform on Escherichia coli biofilm isolated from urinary tract infection. Rosemary and Withania somnifera extracts were obtained by using rotary devices. E. coli was collected from the patients being cured in Zabol hospitals. The least hindering and killing concentration of the samples were calculated by lowering their densityin sinks. The growth and biofilm formation of strains were determined by microtiterplate method. Data were analyzed statistically by determination of significant difference using analysis of variance (ANOVA) test. All tests were analyzed at the significance level P less than 0.05. The results revealed that the concentrations of 5 and 10 mg/mL are the most restrain in the biofilm formation of the isolated plates. Rosemary and Withania somnifera extracts have considerable antimicrobial and ant biofilm effect on the samples of the E. coli resistant to antibiotics.

scholarly journals Biofilm formation byAcinetobacter baumanniistrains isolated from urinary tract infection and urinary catheters

2011 ◽  
Vol 62 (3) ◽  
pp. 328-338 ◽  
Author(s):  
Nadia Kazemi Pour ◽  
Devendra H. Dusane ◽  
Prashant K. Dhakephalkar ◽  
Farokh Rokhbakhsh Zamin ◽  
Smita S. Zinjarde ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Biofilm Formation ◽  
Urinary Catheters

Investigation of Biofilm-Inhibiting Zinc-Silver Oxide Urinary Catheter Coatings

2020 ◽  
Author(s):  
Simonas Vaitkus ◽  
Rafaela Simoes-Torigoe ◽  
Oren Gotlib ◽  
Karcher Morris ◽  
Frederick E. Spada ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Silver Oxide ◽  
Urinary Catheter ◽  
Antimicrobial Properties ◽  
Potential Treatment ◽  
Urinary Catheters ◽  
Biofilm Growth ◽  
Silver Silver

Abstract Catheter associated urinary tract infection (CAUTI) is a widespread complication within hospitals and nursing homes. A potential treatment is to use a biofilm-inhibiting catheter. In this paper, methods for creating a biofilm retardant catheter coating using silver, silver oxide, and/or zinc particles combined with polydimethylsiloxane (PDMS) are described. The coating was first applied to the outside surface of the catheter and then tested for its antimicrobial properties with respect to preventing planktonic and biofilm growth of Escherichia coli. A comparison of our “in house” created catheters was then made with commercially available antimicrobial urinary catheters.

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